Standard name Systematic name Name description Description
VAC14 YLR386W Enzyme regulator Enzyme regulator; involved in synthesis of phosphatidylinositol 3,5-bisphosphate, in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; binds negative (Fig4p) and positive (Fab1p) regulators of PtdIns(3,5)P(2) to control endolysosome function; similar to mammalian Vac14p
Visualize the interactome of VAC14
Links to external databases:
  SGD   |   KEGG   |  

Cell cycle phase and timing of transcription peak

Cell cycle phase Time (min)
G1/S 46

CYCLoPs localization & abundance

WT1 WT2 WT3
Cytoplasm 0.124 0.132 0.113
Endosome 0.313 0.155 0.383
Mito 0.088 0.093 NaN
Nucleolus 0.051 NaN NaN
Vac/Vac Memb 0.539 0.705 0.661

GFP localization & abundance

GFP localization GFP abundance
vacuole 12400

GO term count

Metabolism Cell cycle Cell division Signal transduction DNA replication
VAC14 5 0 0 0 0

Enzyme information

Catalyzed reactions in Yeast8
r_3221 (PI 3,5-P2 phosphatase (1-16:1, 2-18:1), vacuolar membrane), r_3217 (PI 3,5-P2 phosphatase (1-16:1, 2-16:1), vacuolar membrane), r_3216 (PI 3,5-P2 phosphatase (1-16:0, 2-16:1), vacuolar membrane), r_3220 (PI 3,5-P2 phosphatase (1-16:0, 2-18:1), vacuolar membrane), r_3223 (PI 3,5-P2 phosphatase (1-18:1, 2-18:1), vacuolar membrane), r_3222 (PI 3,5-P2 phosphatase (1-18:0, 2-18:1), vacuolar membrane), r_3219 (PI 3,5-P2 phosphatase (1-18:1, 2-16:1), vacuolar membrane), r_3218 (PI 3,5-P2 phosphatase (1-18:0, 2-16:1), vacuolar membrane)

List of interactors

Use the search utility to find the interactor you are looking for. By clicking the column headers the table will be sorted on that column. Use shift+click to sort on multiple columns. Default sorting is on number of experiments, number of unique methods and the number of publications, and alphabetical on standard name, in that order.
Standard name Systematic name Name description Type #Experiments #Methods #Publications Evidence
FIG4 YNL325C Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase physical 8 4 6 Affinity Capture-Western, Affinity Capture-MS, Two-hybrid, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-Western, Affinity Capture-Western
SLT2 YHR030C Serine/threonine MAP kinase genetic 8 2 6 Synthetic Growth Defect, Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic
VAC14 YLR386W Enzyme regulator physical 8 4 5 Two-hybrid, Two-hybrid, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex
FAB1 YFR019W 1-phosphatidylinositol-3-phosphate 5-kinase physical 8 4 4 Affinity Capture-Western, Affinity Capture-Western, Two-hybrid, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-MS, Affinity Capture-Western
SET2 YJL168C Histone methyltransferase with a role in transcriptional elongation genetic 6 3 5 Synthetic Lethality, Synthetic Lethality, Synthetic Growth Defect, Synthetic Growth Defect, Negative Genetic, Negative Genetic
YKE2 YLR200W Subunit of the heterohexameric Gim/prefoldin protein complex genetic 6 2 5 Synthetic Growth Defect, Negative Genetic, Negative Genetic, Synthetic Growth Defect, Negative Genetic, Negative Genetic
BCK1 YJL095W MAPKKK acting in the protein kinase C signaling pathway genetic 6 1 5 Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic
ATG18 YFR021W Phosphoinositide binding protein genetic 6 3 4 Synthetic Growth Defect, Negative Genetic, Phenotypic Suppression, Negative Genetic, Negative Genetic, Negative Genetic
GIM3 YNL153C Subunit of the heterohexameric cochaperone prefoldin complex genetic 5 2 4 Synthetic Growth Defect, Synthetic Growth Defect, Negative Genetic, Negative Genetic, Negative Genetic
VPS29 YHR012W Subunit of the membrane-associated retromer complex genetic 5 2 4 Synthetic Growth Defect, Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic
VPS35 YJL154C Endosomal subunit of membrane-associated retromer complex genetic 5 2 4 Synthetic Growth Defect, Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic
VPS30 YPL120W Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II genetic 5 1 4 Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic
ASE1 YOR058C Mitotic spindle midzone-localized microtubule bundling protein genetic 5 2 3 Synthetic Lethality, Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic
LSM1 YJL124C Lsm (Like Sm) protein genetic 5 1 3 Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic
CLA4 YNL298W Cdc42p-activated signal transducing kinase genetic 4 2 4 Synthetic Lethality, Negative Genetic, Synthetic Lethality, Negative Genetic
MNN10 YDR245W Subunit of a Golgi mannosyltransferase complex genetic 4 2 3 Synthetic Growth Defect, Negative Genetic, Negative Genetic, Negative Genetic
DIA2 YOR080W Origin-binding F-box protein genetic 4 2 3 Synthetic Growth Defect, Negative Genetic, Negative Genetic, Negative Genetic
PAT1 YCR077C Deadenylation-dependent mRNA-decapping factor genetic 4 1 2 Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic
BEM2 YER155C Rho GTPase activating protein (RhoGAP) genetic 4 1 2 Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic
CHK1 YBR274W Serine/threonine kinase and DNA damage checkpoint effector genetic 4 1 2 Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic
VPS51 YKR020W Component of the GARP (Golgi-associated retrograde protein) complex genetic 4 1 2 Positive Genetic, Positive Genetic, Positive Genetic, Positive Genetic
BNI1 YNL271C Formin genetic 3 2 3 Synthetic Growth Defect, Negative Genetic, Negative Genetic
MYO2 YOR326W Type V myosin motor involved in actin-based transport of cargos genetic 3 2 3 Negative Genetic, Synthetic Growth Defect, Negative Genetic
GIM5 YML094W Subunit of the heterohexameric cochaperone prefoldin complex genetic 3 2 3 Synthetic Lethality, Negative Genetic, Negative Genetic
SWI5 YDR146C Transcription factor that recruits Mediator and Swi/Snf complexes genetic 3 2 3 Positive Genetic, Negative Genetic, Negative Genetic
FAB1 YFR019W 1-phosphatidylinositol-3-phosphate 5-kinase genetic 3 2 2 Dosage Rescue, Dosage Rescue, Phenotypic Suppression
BMH1 YER177W 14-3-3 protein, major isoform genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
SLA1 YBL007C Cytoskeletal protein binding protein genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
MLP1 YKR095W Myosin-like protein associated with the nuclear envelope genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
GET2 YER083C Subunit of the GET complex genetic 3 1 2 Positive Genetic, Positive Genetic, Positive Genetic
SPT2 YER161C Protein involved in negative regulation of transcription genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
BUB3 YOR026W Kinetochore checkpoint WD40 repeat protein genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
IPK1 YDR315C Inositol 1,3,4,5,6-pentakisphosphate 2-kinase genetic 3 1 2 Positive Genetic, Positive Genetic, Positive Genetic
MSN5 YDR335W Karyopherin genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
COG7 YGL005C Component of the conserved oligomeric Golgi complex genetic 3 1 2 Positive Genetic, Positive Genetic, Positive Genetic
ARP6 YLR085C Actin-related protein that binds nucleosomes genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
SSA2 YLL024C HSP70 family ATP-binding protein physical 3 1 2 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
GYP1 YOR070C Cis-golgi GTPase-activating protein (GAP) for yeast Rabs genetic 3 1 2 Positive Genetic, Positive Genetic, Positive Genetic
NUP42 YDR192C FG-nucleoporin component of central core of the nuclear pore complex genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
MNN11 YJL183W Subunit of a Golgi mannosyltransferase complex genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
ATP18 YML081C-A Subunit of the mitochondrial F1F0 ATP synthase genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
YDJ1 YNL064C Type I HSP40 co-chaperone genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
RGL1 YPL066W Regulator of Rho1p signaling, cofactor of Tus1p genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
NVJ3 YDR179W-A Protein with a potential role in tethering ER and vacuoles genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
COX10 YPL172C Heme A:farnesyltransferase genetic 3 1 2 Positive Genetic, Positive Genetic, Positive Genetic
DYN3 YMR299C Dynein light intermediate chain (LIC) genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
AIM14 YGL160W NADPH oxidase localized to the perinuclear ER genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
AVT5 YBL089W Putative transporter genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
GIS3 YLR094C Protein of unknown function genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
UPF3 YGR072W Component of the nonsense-mediated mRNA decay (NMD) pathway genetic 3 1 2 Positive Genetic, Positive Genetic, Positive Genetic
TRM2 YKR056W tRNA methyltransferase genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
CDC28 YBR160W Cyclin-dependent kinase (CDK) catalytic subunit genetic 2 2 2 Synthetic Growth Defect, Dosage Lethality
ATG18 YFR021W Phosphoinositide binding protein physical 2 2 2 PCA, Two-hybrid
PAC10 YGR078C Part of the heteromeric co-chaperone GimC/prefoldin complex genetic 2 2 2 Synthetic Growth Defect, Negative Genetic
LTE1 YAL024C Protein similar to GDP/GTP exchange factors genetic 2 2 2 Synthetic Growth Defect, Negative Genetic
TUB2 YFL037W Beta-tubulin genetic 2 2 2 Synthetic Lethality, Negative Genetic
LST8 YNL006W Protein required for the transport of Gap1p genetic 2 1 2 Negative Genetic, Negative Genetic
COG3 YER157W Essential component of the conserved oligomeric Golgi complex genetic 2 1 2 Positive Genetic, Positive Genetic
CMD1 YBR109C Calmodulin genetic 2 1 2 Negative Genetic, Negative Genetic
OPI1 YHL020C Transcriptional regulator of a variety of genes genetic 2 1 2 Negative Genetic, Negative Genetic
ANP1 YEL036C Subunit of the alpha-1,6 mannosyltransferase complex genetic 2 1 2 Negative Genetic, Negative Genetic
SUP45 YBR143C Polypeptide release factor (eRF1) in translation termination physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
MKS1 YNL076W Pleiotropic negative transcriptional regulator genetic 2 1 2 Negative Genetic, Negative Genetic
RGP1 YDR137W Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) genetic 2 1 2 Positive Genetic, Positive Genetic
ENV7 YPL236C Vacuolar membrane protein kinase genetic 2 1 2 Negative Genetic, Negative Genetic
SHP1 YBL058W UBX domain-containing substrate adaptor for Cdc48p genetic 2 1 2 Negative Genetic, Negative Genetic
CDC60 YPL160W Cytosolic leucyl tRNA synthetase genetic 2 1 2 Negative Genetic, Negative Genetic
TEF4 YKL081W Gamma subunit of translational elongation factor eEF1B genetic 2 1 2 Negative Genetic, Negative Genetic
ADE6 YGR061C Formylglycinamidine-ribonucleotide (FGAM)-synthetase physical 2 1 2 Two-hybrid, Two-hybrid
BIM1 YER016W Microtubule plus end-tracking protein genetic 2 1 2 Synthetic Lethality, Synthetic Lethality
FAR3 YMR052W Protein of unknown function genetic 2 1 2 Positive Genetic, Positive Genetic
BNI4 YNL233W Targeting subunit for Glc7p protein phosphatase genetic 2 1 2 Negative Genetic, Negative Genetic
PKC1 YBL105C Protein serine/threonine kinase genetic 2 1 2 Negative Genetic, Negative Genetic
AIM7 YDR063W Protein that interacts with Arp2/3 complex genetic 2 1 2 Negative Genetic, Negative Genetic
URE2 YNL229C Nitrogen catabolite repression transcriptional regulator genetic 2 1 2 Negative Genetic, Negative Genetic
COG5 YNL051W Component of the conserved oligomeric Golgi complex genetic 2 1 2 Positive Genetic, Positive Genetic
CBF1 YJR060W Basic helix-loop-helix (bHLH) protein genetic 2 1 2 Positive Genetic, Positive Genetic
SWI6 YLR182W Transcription cofactor genetic 2 1 2 Negative Genetic, Negative Genetic
MDM12 YOL009C Mitochondrial outer membrane protein, ERMES complex subunit genetic 2 1 2 Negative Genetic, Negative Genetic
CBF2 YGR140W Essential kinetochore protein genetic 2 1 2 Negative Genetic, Negative Genetic
PEP8 YJL053W Vacuolar protein component of the retromer genetic 2 1 2 Negative Genetic, Negative Genetic
SNX4 YJL036W Sorting nexin genetic 2 1 2 Positive Genetic, Positive Genetic
MON1 YGL124C Subunit of a heterodimeric guanine nucleotide exchange factor (GEF) genetic 2 1 2 Negative Genetic, Negative Genetic
MTF2 YDL044C Mitochondrial protein that interacts with mitochondrial RNA polymerase physical 2 1 2 Two-hybrid, Two-hybrid
TPM1 YNL079C Major isoform of tropomyosin genetic 2 1 2 Negative Genetic, Negative Genetic
STP1 YDR463W Transcription factor genetic 2 1 2 Negative Genetic, Negative Genetic
VPS73 YGL104C Mitochondrial protein genetic 2 1 2 Positive Genetic, Positive Genetic
SLM3 YDL033C tRNA-specific 2-thiouridylase genetic 2 1 2 Negative Genetic, Negative Genetic
SCT1 YBL011W Glycerol 3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase genetic 2 1 2 Negative Genetic, Negative Genetic
RUD3 YOR216C Golgi matrix protein genetic 2 1 2 Positive Genetic, Positive Genetic
TOM71 YHR117W Mitochondrial outer membrane protein genetic 2 1 2 Negative Genetic, Negative Genetic
RPL14A YKL006W Ribosomal 60S subunit protein L14A genetic 2 1 2 Negative Genetic, Negative Genetic
PDR3 YBL005W Transcriptional activator of the pleiotropic drug resistance network genetic 2 1 2 Negative Genetic, Negative Genetic
FSH3 YOR280C Putative serine hydrolase genetic 2 1 2 Negative Genetic, Negative Genetic
CRD1 YDL142C Cardiolipin synthase genetic 2 1 2 Negative Genetic, Negative Genetic
TSC11 YER093C Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p) genetic 2 1 2 Negative Genetic, Negative Genetic
VAC7 YNL054W Integral vacuolar membrane protein physical 2 2 1 Two-hybrid, Affinity Capture-Western
TRM3 YDL112W 2'-O-ribose methyltransferase genetic 2 1 1 Negative Genetic, Negative Genetic
EAF3 YPR023C Component of the Rpd3S histone deacetylase complex genetic 2 1 1 Negative Genetic, Negative Genetic
MDM10 YAL010C Subunit of both the ERMES and the SAM complex genetic 2 1 1 Negative Genetic, Negative Genetic
TLG2 YOL018C Syntaxin-like t-SNARE genetic 2 1 1 Positive Genetic, Positive Genetic
GET3 YDL100C Guanine nucleotide exchange factor for Gpa1p genetic 2 1 1 Positive Genetic, Positive Genetic
APM3 YBR288C Mu3-like subunit of the clathrin associated protein complex (AP-3) genetic 2 1 1 Positive Genetic, Positive Genetic
SIW14 YNL032W Inositol phosphatase involved in inositol pyrophosphate metabolism genetic 2 1 1 Negative Genetic, Negative Genetic
TAF9 YMR236W Subunit (17 kDa) of TFIID and SAGA complexes genetic 2 1 1 Synthetic Lethality, Synthetic Lethality
PEX31 YGR004W Peroxisomal integral membrane protein genetic 2 1 1 Negative Genetic, Negative Genetic
LDB18 YLL049W Component of the dynactin complex genetic 2 1 1 Negative Genetic, Negative Genetic
EMI1 YDR512C Non-essential protein of unknown function genetic 2 1 1 Negative Genetic, Negative Genetic
PRM7 YDL039C Pheromone-regulated protein genetic 2 1 1 Negative Genetic, Negative Genetic
SPE1 YKL184W Ornithine decarboxylase genetic 2 1 1 Negative Genetic, Negative Genetic
ICE2 YIL090W Integral ER membrane protein with type-III transmembrane domains genetic 2 1 1 Negative Genetic, Negative Genetic
PPM1 YDR435C Carboxyl methyltransferase genetic 2 1 1 Negative Genetic, Negative Genetic
FKH1 YIL131C Forkhead family transcription factor regulation 1 1 1 chromatin immunoprecipitation-chip evidence
SFP1 YLR403W Regulates transcription of ribosomal protein and biogenesis genes regulation 1 1 1 DNA to cDNA expression microarray evidence
MED2 YDL005C Subunit of the RNA polymerase II mediator complex regulation 1 1 1 chromatin immunoprecipitation-chip evidence
XBP1 YIL101C Transcriptional repressor regulation 1 1 1 chromatin immunoprecipitation-chip evidence
YAP6 YDR259C Basic leucine zipper (bZIP) transcription factor regulation 1 1 1 chromatin immunoprecipitation-chip evidence
TFC7 YOR110W RNA pol III transcription initiation factor complex (TFIIIC) subunit regulation 1 1 1 chromatin immunoprecipitation-chip evidence
LEU3 YLR451W Zinc-knuckle transcription factor, repressor and activator regulation 1 1 1 chromatin immunoprecipitation-chip evidence
MBP1 YDL056W Transcription factor regulation 1 1 1 chromatin immunoprecipitation-chip evidence
NSE3 YDR288W Component of the SMC5-SMC6 complex genetic 1 1 1 Positive Genetic
SWC4 YGR002C Component of the Swr1p complex that incorporates Htz1p into chromatin genetic 1 1 1 Negative Genetic
RLP7 YNL002C Nucleolar protein similar to large ribosomal subunit L7 proteins genetic 1 1 1 Negative Genetic
BRR2 YER172C RNA-dependent ATPase RNA helicase (DEIH box) genetic 1 1 1 Synthetic Growth Defect
PRP43 YGL120C RNA helicase in the DEAH-box family genetic 1 1 1 Negative Genetic
PRP45 YAL032C Protein required for pre-mRNA splicing physical 1 1 1 Two-hybrid
PRP8 YHR165C Component of U4/U6-U5 snRNP complex genetic 1 1 1 Negative Genetic
GUS1 YGL245W Glutamyl-tRNA synthetase (GluRS) genetic 1 1 1 Negative Genetic
MDM20 YOL076W Non-catalytic subunit of the NatB N-terminal acetyltransferase genetic 1 1 1 Negative Genetic
LSM6 YDR378C Lsm (Like Sm) protein genetic 1 1 1 Negative Genetic
CDC48 YDL126C AAA ATPase with protein-unfoldase activity genetic 1 1 1 Negative Genetic
NPL3 YDR432W RNA-binding protein genetic 1 1 1 Synthetic Growth Defect
RPC25 YKL144C RNA polymerase III subunit C25 genetic 1 1 1 Positive Genetic
RPA49 YNL248C RNA polymerase I subunit A49 genetic 1 1 1 Negative Genetic
SIN4 YNL236W Subunit of the RNA polymerase II mediator complex genetic 1 1 1 Positive Genetic
SNP1 YIL061C Component of U1 snRNP required for mRNA splicing via spliceosome physical 1 1 1 Affinity Capture-MS
RPN5 YDL147W Subunit of the CSN and 26S proteasome lid complexes genetic 1 1 1 Negative Genetic
RPN9 YDR427W Non-ATPase regulatory subunit of the 26S proteasome physical 1 1 1 Two-hybrid
RPT2 YDL007W ATPase of the 19S regulatory particle of the 26S proteasome genetic 1 1 1 Negative Genetic
ACT1 YFL039C Actin, structural protein in cytoskeleton genetic 1 1 1 Negative Genetic
CFT2 YLR115W Subunit of the mRNA cleavage and polyadenlylation factor (CPF) genetic 1 1 1 Negative Genetic
MPE1 YKL059C Subunit of CPF cleavage and polyadenylation factor and E3 Ub-ligase genetic 1 1 1 Negative Genetic
SSD1 YDR293C Translational repressor genetic 1 1 1 Negative Genetic
PRE3 YJL001W Beta 1 subunit of the 20S proteasome physical 1 1 1 Two-hybrid
PRE8 YML092C Alpha 2 subunit of the 20S proteasome physical 1 1 1 Two-hybrid
CCR4 YAL021C Component of the CCR4-NOT transcriptional complex physical 1 1 1 Affinity Capture-RNA
CCR4 YAL021C Component of the CCR4-NOT transcriptional complex genetic 1 1 1 Synthetic Growth Defect
ACC1 YNR016C Acetyl-CoA carboxylase, biotin containing enzyme physical 1 1 1 Affinity Capture-MS
APC1 YNL172W Largest subunit of the Anaphase-Promoting Complex/Cyclosome genetic 1 1 1 Positive Genetic
APL2 YKL135C Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex genetic 1 1 1 Negative Genetic
CAF40 YNL288W Component of the CCR4-NOT transcriptional complex genetic 1 1 1 Negative Genetic
CDC14 YFR028C Protein phosphatase required for mitotic exit genetic 1 1 1 Negative Genetic
CDC36 YDL165W Component of the CCR4-NOT core complex, involved in mRNA decapping genetic 1 1 1 Negative Genetic
CDC55 YGL190C Regulatory subunit B of protein phosphatase 2A (PP2A) genetic 1 1 1 Negative Genetic
BUD31 YCR063W Component of the SF3b subcomplex of the U2 snRNP genetic 1 1 1 Negative Genetic
AVO1 YOL078W Component of the membrane-bound TORC2 complex genetic 1 1 1 Negative Genetic
COP1 YDL145C Alpha subunit of COPI vesicle coatomer complex genetic 1 1 1 Positive Genetic
CYR1 YJL005W Adenylate cyclase genetic 1 1 1 Negative Genetic
GCD7 YLR291C Beta subunit of translation initiation factor eIF2B physical 1 1 1 Two-hybrid
ESP1 YGR098C Separase/separin, a caspase-like cysteine protease genetic 1 1 1 Synthetic Growth Defect
DYN1 YKR054C Cytoplasmic heavy chain dynein genetic 1 1 1 Negative Genetic
COG8 YML071C Component of the conserved oligomeric Golgi complex genetic 1 1 1 Positive Genetic
ISW1 YBR245C ATPase subunit of imitation-switch (ISWI) class chromatin remodelers physical 1 1 1 Affinity Capture-RNA
HSL1 YKL101W Nim1p-related protein kinase genetic 1 1 1 Negative Genetic
LAS17 YOR181W Actin assembly factor genetic 1 1 1 Negative Genetic
LSM4 YER112W Lsm (Like Sm) protein physical 1 1 1 Two-hybrid
MHR1 YDR296W Mitochondrial ribosomal protein of the large subunit physical 1 1 1 Two-hybrid
ARP8 YOR141C Nuclear actin-related protein involved in chromatin remodeling genetic 1 1 1 Negative Genetic
APM1 YPL259C Mu1-like medium subunit of the AP-1 complex genetic 1 1 1 Negative Genetic
LPD1 YFL018C Dihydrolipoamide dehydrogenase genetic 1 1 1 Negative Genetic
POP2 YNR052C Subunit of Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation genetic 1 1 1 Synthetic Growth Defect
HSP82 YPL240C Hsp90 chaperone genetic 1 1 1 Synthetic Lethality
GLE2 YER107C RNA export factor associated with the nuclear pore complex (NPC) genetic 1 1 1 Negative Genetic
DAM1 YGR113W Essential subunit of the Dam1 complex (aka DASH complex) genetic 1 1 1 Negative Genetic
POL2 YNL262W Catalytic subunit of DNA polymerase (II) epsilon genetic 1 1 1 Negative Genetic
ILV1 YER086W Threonine deaminase, catalyzes first step in isoleucine biosynthesis genetic 1 1 1 Positive Genetic
RAD52 YML032C Protein involved in homologous recombination genetic 1 1 1 Positive Genetic
FYV8 YGR196C Protein of unknown function genetic 1 1 1 Negative Genetic
IWR1 YDL115C RNA polymerase II transport factor, conserved from yeast to humans genetic 1 1 1 Positive Genetic
DEP1 YAL013W Component of the Rpd3L histone deacetylase complex genetic 1 1 1 Positive Genetic
RRI1 YDL216C Catalytic subunit of the COP9 signalosome (CSN) complex genetic 1 1 1 Positive Genetic
NUP120 YKL057C Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC) genetic 1 1 1 Negative Genetic
SEC63 YOR254C Essential subunit of Sec63 complex genetic 1 1 1 Negative Genetic
CSE1 YGL238W Nuclear envelope protein that acts as a recycling factor genetic 1 1 1 Negative Genetic
SDS3 YIL084C Component of the Rpd3L histone deacetylase complex genetic 1 1 1 Negative Genetic
SIN3 YOL004W Component of both the Rpd3S and Rpd3L histone deacetylase complexes genetic 1 1 1 Positive Genetic
CCT2 YIL142W Subunit beta of the cytosolic chaperonin Cct ring complex genetic 1 1 1 Negative Genetic
RPT5 YOR117W ATPase of the 19S regulatory particle of the 26S proteasome genetic 1 1 1 Negative Genetic
TAF11 YML015C TFIID subunit (40 kDa) genetic 1 1 1 Negative Genetic
PTC1 YDL006W Type 2C protein phosphatase (PP2C) genetic 1 1 1 Positive Genetic
TAF6 YGL112C Subunit (60 kDa) of TFIID and SAGA complexes genetic 1 1 1 Negative Genetic
CCT6 YDR188W Subunit of the cytosolic chaperonin Cct ring complex genetic 1 1 1 Negative Genetic
TEF2 YBR118W Translational elongation factor EF-1 alpha physical 1 1 1 Affinity Capture-MS
TFB1 YDR311W Subunit of TFIIH and nucleotide excision repair factor 3 complexes genetic 1 1 1 Positive Genetic
BET5 YML077W Core component of transport protein particle (TRAPP) complexes I-III genetic 1 1 1 Positive Genetic
TRS20 YBR254C Core component of transport protein particle (TRAPP) complexes I-III genetic 1 1 1 Positive Genetic
HSE1 YHL002W Subunit of the endosomal Vps27p-Hse1p complex genetic 1 1 1 Negative Genetic
DRS2 YAL026C Trans-golgi network aminophospholipid translocase (flippase) genetic 1 1 1 Positive Genetic
SNT1 YCR033W Set3C histone deacetylase scaffold genetic 1 1 1 Negative Genetic
CDC13 YDL220C Single stranded DNA-binding protein found at TG1-3 telomere G-tails genetic 1 1 1 Positive Genetic
BBC1 YJL020C Protein possibly involved in assembly of actin patches physical 1 1 1 Affinity Capture-MS
PHO85 YPL031C Cyclin-dependent kinase genetic 1 1 1 Synthetic Growth Defect
BUD13 YGL174W Subunit of the RES complex genetic 1 1 1 Negative Genetic
GET1 YGL020C Subunit of the GET complex genetic 1 1 1 Positive Genetic
DUN1 YDL101C Cell-cycle checkpoint S/T protein kinase genetic 1 1 1 Negative Genetic
GLC8 YMR311C Regulatory subunit of protein phosphatase 1 (Glc7p) genetic 1 1 1 Negative Genetic
CDC9 YDL164C DNA ligase I found in nucleus and mitochondria genetic 1 1 1 Positive Genetic
CLN1 YMR199W G1 cyclin involved in regulation of the cell cycle genetic 1 1 1 Negative Genetic
COF1 YLL050C Cofilin, involved in pH-dependent actin filament depolarization genetic 1 1 1 Negative Genetic
ILV3 YJR016C Dihydroxyacid dehydratase genetic 1 1 1 Negative Genetic
COS111 YBR203W Protein required for antifungal drug ciclopirox olamine resistance genetic 1 1 1 Negative Genetic
KNS1 YLL019C Protein kinase involved in negative regulation of PolIII transcription genetic 1 1 1 Negative Genetic
SGS1 YMR190C RecQ family nucleolar DNA helicase genetic 1 1 1 Positive Genetic
RSP5 YER125W NEDD4 family E3 ubiquitin ligase genetic 1 1 1 Negative Genetic
OCA1 YNL099C Putative protein tyrosine phosphatase genetic 1 1 1 Negative Genetic
LTP1 YPR073C Protein phosphotyrosine phosphatase of unknown cellular role genetic 1 1 1 Positive Genetic
MAK11 YKL021C Protein involved in an early step of 60S ribosomal subunit biogenesis genetic 1 1 1 Positive Genetic
TEM1 YML064C GTPase involved in initiation of Mitotic Exit Network (MEN) genetic 1 1 1 Negative Genetic
CDC15 YAR019C Hippo-like kinase of the Mitotic Exit Network genetic 1 1 1 Negative Genetic
DPB11 YJL090C DNA replication initiation protein physical 1 1 1 Two-hybrid
CDC123 YLR215C Assembly factor for the eIF2 translation initiation factor complex physical 1 1 1 Two-hybrid
OMS1 YDR316W Protein integral to the mitochondrial membrane genetic 1 1 1 Negative Genetic
AIR1 YIL079C Zinc knuckle protein genetic 1 1 1 Negative Genetic
TPK3 YKL166C cAMP-dependent protein kinase catalytic subunit genetic 1 1 1 Phenotypic Suppression
DBF2 YGR092W Ser/Thr kinase involved in transcription and stress response genetic 1 1 1 Negative Genetic
CLN2 YPL256C G1 cyclin involved in regulation of the cell cycle physical 1 1 1 Affinity Capture-MS
CLN2 YPL256C G1 cyclin involved in regulation of the cell cycle genetic 1 1 1 Negative Genetic
PMI40 YER003C Mannose-6-phosphate isomerase genetic 1 1 1 Negative Genetic
GCR2 YNL199C Transcriptional activator of genes involved in glycolysis genetic 1 1 1 Positive Genetic
MDH3 YDL078C Peroxisomal malate dehydrogenase genetic 1 1 1 Negative Genetic
HFL1 YKR051W Vacuole membrane protein that interacts with Atg8p genetic 1 1 1 Positive Genetic
CDC7 YDL017W S/T kinase and catalytic subunit of DDK (Dbf4-dependent kinase) physical 1 1 1 Affinity Capture-MS
CDC7 YDL017W S/T kinase and catalytic subunit of DDK (Dbf4-dependent kinase) genetic 1 1 1 Negative Genetic
ERG11 YHR007C Lanosterol 14-alpha-demethylase genetic 1 1 1 Negative Genetic
SNF4 YGL115W Activating gamma subunit of the AMP-activated Snf1p kinase complex genetic 1 1 1 Negative Genetic
SRV2 YNL138W CAP (cyclase-associated protein) genetic 1 1 1 Negative Genetic
CTK3 YML112W Gamma subunit of C-terminal domain kinase I genetic 1 1 1 Negative Genetic
TOP1 YOL006C Topoisomerase I genetic 1 1 1 Negative Genetic
PUT3 YKL015W Transcriptional activator genetic 1 1 1 Negative Genetic
GPI16 YHR188C Subunit of the glycosylphosphatidylinositol transamidase complex genetic 1 1 1 Positive Genetic
SMC5 YOL034W Subunit of the SMC5-SMC6 complex genetic 1 1 1 Negative Genetic
OCA2 YNL056W Protein of unknown function genetic 1 1 1 Negative Genetic
AIM4 YBR194W Protein proposed to be associated with the nuclear pore complex genetic 1 1 1 Negative Genetic
COG6 YNL041C Component of the conserved oligomeric Golgi complex physical 1 1 1 Two-hybrid
SAC3 YDR159W mRNA export factor genetic 1 1 1 Negative Genetic
SUS1 YBR111W-A Global transcriptional regulator, component of SAGA, TREX-2 complexes genetic 1 1 1 Negative Genetic
IES2 YNL215W Protein that associates with the INO80 chromatin remodeling complex genetic 1 1 1 Negative Genetic
SWI3 YJL176C Subunit of the SWI/SNF chromatin remodeling complex genetic 1 1 1 Negative Genetic
RTG3 YBL103C bHLH/Zip transcription factor for retrograde (RTG) and TOR pathways genetic 1 1 1 Negative Genetic
PLC1 YPL268W Phospholipase C physical 1 1 1 Two-hybrid
ATG8 YBL078C Ub-like protein genetic 1 1 1 Negative Genetic
BUB1 YGR188C Protein kinase involved in the cell cycle checkpoint into anaphase genetic 1 1 1 Negative Genetic
CLC1 YGR167W Clathrin light chain genetic 1 1 1 Positive Genetic
CTK2 YJL006C Beta subunit of C-terminal domain kinase I (CTDK-I) genetic 1 1 1 Negative Genetic
GIM4 YEL003W Subunit of the heterohexameric cochaperone prefoldin complex genetic 1 1 1 Synthetic Growth Defect
GEA1 YJR031C Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs) genetic 1 1 1 Negative Genetic
SEC61 YLR378C Conserved ER protein translocation channel physical 1 1 1 Affinity Capture-MS
MPS2 YGL075C Essential membrane protein localized at nuclear envelope and SPBs physical 1 1 1 Two-hybrid
SSA1 YAL005C ATPase involved in protein folding and NLS-directed nuclear transport physical 1 1 1 Affinity Capture-MS
TUB4 YLR212C Gamma-tubulin genetic 1 1 1 Synthetic Rescue
NMD2 YHR077C Protein involved in the nonsense-mediated mRNA decay (NMD) pathway genetic 1 1 1 Positive Genetic
HSF1 YGL073W Trimeric heat shock transcription factor genetic 1 1 1 Negative Genetic
SPO20 YMR017W Meiosis-specific subunit of the t-SNARE complex genetic 1 1 1 Negative Genetic
SRM1 YGL097W Nucleotide exchange factor for Gsp1p genetic 1 1 1 Negative Genetic
SLN1 YIL147C Transmembrane histidine phosphotransfer kinase and osmosensor genetic 1 1 1 Negative Genetic
ESC2 YDR363W Sumo-like domain protein genetic 1 1 1 Positive Genetic
INP53 YOR109W Polyphosphatidylinositol phosphatase genetic 1 1 1 Positive Genetic
BCH1 YMR237W Member of the ChAPs family (Chs5p-Arf1p-binding proteins) genetic 1 1 1 Negative Genetic
REC107 YJR021C Protein involved in early stages of meiotic recombination physical 1 1 1 Two-hybrid
MET28 YIR017C bZIP transcriptional activator in the Cbf1p-Met4p-Met28p complex genetic 1 1 1 Negative Genetic
NNF2 YGR089W Protein that exhibits physical and genetic interactions with Rpb8p genetic 1 1 1 Negative Genetic
PRP22 YER013W DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase genetic 1 1 1 Negative Genetic
SPC42 YKL042W Central plaque component of spindle pole body (SPB) genetic 1 1 1 Positive Genetic
OSH3 YHR073W Member of an oxysterol-binding protein family genetic 1 1 1 Negative Genetic
STU2 YLR045C Microtubule polymerase of the XMAP215/Dis1 family genetic 1 1 1 Negative Genetic
PFY1 YOR122C Profilin genetic 1 1 1 Negative Genetic
TOR1 YJR066W PIK-related protein kinase and rapamycin target genetic 1 1 1 Negative Genetic
HRD1 YOL013C Ubiquitin-protein ligase involved in ER-associated degradation (ERAD) genetic 1 1 1 Negative Genetic
BET4 YJL031C Alpha subunit of Type II geranylgeranyltransferase genetic 1 1 1 Negative Genetic
ARL3 YPL051W ARF-like small GTPase of the RAS superfamily genetic 1 1 1 Positive Genetic
EAF7 YNL136W Subunit of the NuA4 histone acetyltransferase complex genetic 1 1 1 Negative Genetic
RPL28 YGL103W Ribosomal 60S subunit protein L28 genetic 1 1 1 Positive Genetic
TFB5 YDR079C-A Component of RNA polymerase II general transcription factor TFIIH genetic 1 1 1 Negative Genetic
INN1 YNL152W Essential protein that associates with contractile actomyosin ring genetic 1 1 1 Negative Genetic
TEC1 YBR083W Transcription factor targeting filamentation genes and Ty1 expression genetic 1 1 1 Positive Genetic
SLK19 YOR195W Kinetochore-associated protein genetic 1 1 1 Negative Genetic
GPI2 YPL076W Protein involved in the synthesis of GlcNAc-PI genetic 1 1 1 Positive Genetic
RPL40B YKR094C Ubiquitin-ribosomal 60S subunit protein L40B fusion protein genetic 1 1 1 Positive Genetic
HRQ1 YDR291W 3'-5' DNA helicase of the conserved RecQ family genetic 1 1 1 Positive Genetic
KCS1 YDR017C Inositol hexakisphosphate and inositol heptakisphosphate kinase genetic 1 1 1 Negative Genetic
MSS4 YDR208W Phosphatidylinositol-4-phosphate 5-kinase genetic 1 1 1 Negative Genetic
SCC4 YER147C Subunit of cohesin loading factor (Scc2p-Scc4p) genetic 1 1 1 Negative Genetic
BRL1 YHR036W Essential nuclear envelope/ER integral membrane protein genetic 1 1 1 Negative Genetic
MSS1 YMR023C Mitochondrial protein genetic 1 1 1 Negative Genetic
OCA4 YCR095C Cytoplasmic protein required for replication of Brome mosaic virus genetic 1 1 1 Negative Genetic
BYE1 YKL005C Negative regulator of transcription elongation genetic 1 1 1 Negative Genetic
RIA1 YNL163C Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis genetic 1 1 1 Positive Genetic
SMT1 YJL147C Translational repressor of the mitochondrial ATP6/8 mRNA genetic 1 1 1 Negative Genetic
YRA1 YDR381W Nuclear polyadenylated RNA-binding protein physical 1 1 1 Affinity Capture-MS
VAM6 YDL077C Guanine nucleotide exchange factor for the GTPase Gtr1p physical 1 1 1 Affinity Capture-MS
VMA1 YDL185W Subunit A of the V1 peripheral membrane domain of V-ATPase genetic 1 1 1 Synthetic Growth Defect
VPS3 YDR495C Component of CORVET membrane tethering complex genetic 1 1 1 Negative Genetic
YGL235W YGL235W Putative protein of unknown function genetic 1 1 1 Negative Genetic
VMA21 YGR105W Integral membrane protein required for V-ATPase function genetic 1 1 1 Synthetic Growth Defect
VOA1 YGR106C ER protein that functions in assembly of the V0 sector of V-ATPase genetic 1 1 1 Synthetic Growth Defect
YKU70 YMR284W Subunit of the telomeric Ku complex (Yku70p-Yku80p) genetic 1 1 1 Negative Genetic
VMA9 YCL005W-A Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex genetic 1 1 1 Positive Genetic
VAC8 YEL013W Vacuole-specific Myo2p receptor genetic 1 1 1 Positive Genetic
YFR057W YFR057W Putative protein of unknown function genetic 1 1 1 Positive Genetic
VAM10 YOR068C Protein involved in vacuole morphogenesis genetic 1 1 1 Positive Genetic
VMA13 YPR036W Subunit H of the V1 peripheral membrane domain of V-ATPase genetic 1 1 1 Positive Genetic
WRS1 YOL097C Cytoplasmic tryptophanyl-tRNA synthetase genetic 1 1 1 Negative Genetic
YER079W YER079W Putative protein of unknown function genetic 1 1 1 Negative Genetic
YHR045W YHR045W Putative protein of unknown function genetic 1 1 1 Negative Genetic
VPS72 YDR485C Htz1p-binding component of the SWR1 complex genetic 1 1 1 Negative Genetic
WHI2 YOR043W Negative regulator of TORC1 in response to limiting leucine genetic 1 1 1 Negative Genetic
YKL091C YKL091C Putative phosphatidylinositol/phosphatidylcholine transfer protein genetic 1 1 1 Positive Genetic
VPH1 YOR270C Subunit a of the vacuolar-ATPase V0 domain genetic 1 1 1 Synthetic Rescue
VNX1 YNL321W Calcium/H+ antiporter localized to the endoplasmic reticulum membrane genetic 1 1 1 Synthetic Rescue
UBP14 YBR058C Ubiquitin-specific protease genetic 1 1 1 Negative Genetic
POM33 YLL023C Transmembrane nucleoporin genetic 1 1 1 Negative Genetic
ERP3 YDL018C Protein with similarity to Emp24p and Erv25p genetic 1 1 1 Negative Genetic
GSH1 YJL101C Gamma glutamylcysteine synthetase genetic 1 1 1 Positive Genetic
DPH5 YLR172C Methyltransferase required for synthesis of diphthamide genetic 1 1 1 Negative Genetic
OCA5 YHL029C Cytoplasmic protein required for replication of Brome mosaic virus genetic 1 1 1 Negative Genetic
FPS1 YLL043W Aquaglyceroporin, plasma membrane channel genetic 1 1 1 Negative Genetic
AIM11 YER093C-A Protein of unknown function genetic 1 1 1 Negative Genetic
DER1 YBR201W ER membrane protein that promotes export of misfolded polypeptides genetic 1 1 1 Negative Genetic
RRT2 YBR246W Methylesterase performing penultimate step of diphthamide biosynthesis genetic 1 1 1 Negative Genetic
TED1 YIL039W GPI-glycan remodelase genetic 1 1 1 Negative Genetic
CHS2 YBR038W Chitin synthase II genetic 1 1 1 Negative Genetic
SHE4 YOR035C Protein containing a UCS (UNC-45/CRO1/SHE4) domain genetic 1 1 1 Negative Genetic
MSA1 YOR066W Activator of G1-specific transcription factors MBF and SBF genetic 1 1 1 Negative Genetic
GOT1 YMR292W Homodimeric protein that is packaged into COPII vesicles genetic 1 1 1 Negative Genetic
SHR3 YDL212W Endoplasmic reticulum packaging chaperone genetic 1 1 1 Negative Genetic
ERP4 YOR016C Member of the p24 family involved in ER to Golgi transport genetic 1 1 1 Negative Genetic
SIC1 YLR079W Cyclin-dependent kinase inhibitor (CKI) genetic 1 1 1 Negative Genetic
CEM1 YER061C Mitochondrial beta-keto-acyl synthase genetic 1 1 1 Negative Genetic
SFB3 YHR098C Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat genetic 1 1 1 Negative Genetic
KRE1 YNL322C Cell wall glycoprotein involved in beta-glucan assembly genetic 1 1 1 Positive Genetic
BIG1 YHR101C Integral membrane protein of the endoplasmic reticulum genetic 1 1 1 Negative Genetic
MRN1 YPL184C RNA-binding protein that may be involved in translational regulation genetic 1 1 1 Positive Genetic
PAI3 YMR174C Cytoplasmic proteinase A (Pep4p) inhibitor genetic 1 1 1 Negative Genetic
SMF3 YLR034C Putative divalent metal ion transporter involved in iron homeostasis genetic 1 1 1 Negative Genetic
QRI1 YDL103C UDP-N-acetylglucosamine pyrophosphorylase genetic 1 1 1 Negative Genetic
MDM35 YKL053C-A Mitochondrial intermembrane space protein genetic 1 1 1 Negative Genetic
MPC2 YHR162W Highly conserved subunit of the mitochondrial pyruvate carrier (MPC) genetic 1 1 1 Negative Genetic
MRM2 YGL136C Mitochondrial 2' O-ribose methyltransferase genetic 1 1 1 Negative Genetic
DSC2 YOL073C Multi-transmembrane subunit of the DSC ubiquitin ligase complex genetic 1 1 1 Negative Genetic
IMP2 YMR035W Catalytic subunit of mitochondrial inner membrane peptidase complex genetic 1 1 1 Negative Genetic
APS3 YJL024C Small subunit of the clathrin-associated adaptor complex AP-3 genetic 1 1 1 Positive Genetic
SRP102 YKL154W Signal recognition particle (SRP) receptor beta subunit genetic 1 1 1 Positive Genetic
TNA1 YGR260W High affinity nicotinic acid plasma membrane permease genetic 1 1 1 Negative Genetic
IMA1 YGR287C Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase) genetic 1 1 1 Negative Genetic
MET22 YOL064C Bisphosphate-3'-nucleotidase genetic 1 1 1 Positive Genetic
SFM1 YOR021C SPOUT methyltransferase genetic 1 1 1 Negative Genetic
TBS1 YBR150C Protein of unknown function genetic 1 1 1 Negative Genetic
MNT3 YIL014W Alpha-1,3-mannosyltransferase genetic 1 1 1 Negative Genetic
SEC12 YNR026C Guanine nucleotide exchange factor (GEF) genetic 1 1 1 Negative Genetic
SPP41 YDR464W Protein of unknown function genetic 1 1 1 Negative Genetic
SHY1 YGR112W Mitochondrial inner membrane protein required for complex IV assembly genetic 1 1 1 Negative Genetic
PET20 YPL159C Mitochondrial protein genetic 1 1 1 Negative Genetic
DML1 YMR211W Essential protein involved in mtDNA inheritance genetic 1 1 1 Negative Genetic
NPP1 YCR026C Nucleotide pyrophosphatase/phosphodiesterase genetic 1 1 1 Negative Genetic
ECM8 YBR076W Non-essential protein of unknown function genetic 1 1 1 Negative Genetic
OCA6 YDR067C Cytoplasmic protein required for replication of Brome mosaic virus genetic 1 1 1 Negative Genetic
SUT2 YPR009W Zn2Cys6 family transcription factor genetic 1 1 1 Positive Genetic