Standard name Systematic name Name description Description
DNF3 YMR162C Trans-golgi network aminophospholipid translocase (flippase) Trans-golgi network aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to the maintenance of membrane lipid asymmetry in post-Golgi secretory vesicles; role in protein trafficking between the Golgi and endosomal system; localizes to the trans-Golgi network; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone
Visualize the interactome of DNF3
Links to external databases:
  SGD   |   KEGG   |  

Cell cycle phase and timing of transcription peak

Cell cycle phase Time (min)

CYCLoPs localization & abundance

WT1 WT2 WT3
Cort. Patches 0.206 0.228 NaN
Cytoplasm 0.466 0.441 0.440
ER 0.154 0.194 NaN
Endosome 0.106 NaN 0.197
Mito 0.075 0.219 0.108
SpindlePole 0.066 NaN 0.143
Bud NaN 0.059 0.096
Peroxisome NaN NaN 0.070

GFP localization & abundance

GFP localization GFP abundance
cytoplasm low signal

GO term count

Metabolism Cell cycle Cell division Signal transduction DNA replication
DNF3 0 0 0 0 0

Enzyme information

Catalyzed reactions in Yeast8
r_3819 (phosphatidyl-L-serine (1-18:0, 2-18:1) transport, ER membrane-Golgi membrane), r_3815 (phosphatidyl-L-serine (1-18:0, 2-16:1) transport, ER membrane-Golgi membrane), r_3818 (phosphatidyl-L-serine (1-16:1, 2-18:1) transport, ER membrane-Golgi membrane), r_3820 (phosphatidyl-L-serine (1-18:1, 2-18:1) transport, ER membrane-Golgi membrane), r_3816 (phosphatidyl-L-serine (1-18:1, 2-16:1) transport, ER membrane-Golgi membrane), r_4308 (Probable phospholipid-transporting ATPase DNF3 (EC 3.6.3.1) (Aminophospholipid translocase) (APT) (Phospholipid translocase) (PLT)), r_3814 (phosphatidyl-L-serine (1-16:1, 2-16:1) transport, ER membrane-Golgi membrane), r_3817 (phosphatidyl-L-serine (1-16:0, 2-18:1) transport, ER membrane-Golgi membrane), r_3813 (phosphatidyl-L-serine (1-16:0, 2-16:1) transport, ER membrane-Golgi membrane)

List of interactors

Use the search utility to find the interactor you are looking for. By clicking the column headers the table will be sorted on that column. Use shift+click to sort on multiple columns. Default sorting is on number of experiments, number of unique methods and the number of publications, and alphabetical on standard name, in that order.
Standard name Systematic name Name description Type #Experiments #Methods #Publications Evidence
DNF1 YER166W Aminophospholipid translocase (flippase) genetic 3 2 3 Synthetic Rescue, Phenotypic Enhancement, Phenotypic Enhancement
FPK1 YNR047W Ser/Thr protein kinase regulation 2 2 2 protein kinase assay evidence, qualitative western immunoblotting evidence
DNF2 YDR093W Aminophospholipid translocase (flippase) genetic 2 1 2 Phenotypic Enhancement, Phenotypic Enhancement
ATG15 YCR068W Phospholipase genetic 2 1 2 Negative Genetic, Negative Genetic
PUF3 YLL013C Protein of the mitochondrial outer surface physical 2 1 2 Affinity Capture-RNA, Affinity Capture-RNA
ROM2 YLR371W Guanine nucleotide exchange factor (GEF) for Rho1p and Rho2p genetic 2 1 2 Negative Genetic, Negative Genetic
MSB4 YOL112W GTPase-activating protein of the Ras superfamily genetic 2 1 2 Negative Genetic, Negative Genetic
YPK1 YKL126W S/T protein kinase genetic 2 1 2 Phenotypic Suppression, Phenotypic Suppression
RPP2B YDR382W Ribosomal protein P2 beta genetic 2 1 1 Negative Genetic, Negative Genetic
CCR4 YAL021C Component of the CCR4-NOT transcriptional complex physical 1 1 1 Affinity Capture-RNA
COX5A YNL052W Subunit Va of cytochrome c oxidase genetic 1 1 1 Negative Genetic
DHH1 YDL160C Cytoplasmic DEAD-box helicase and mRNA decapping activator physical 1 1 1 Affinity Capture-RNA
BZZ1 YHR114W SH3 domain protein implicated in regulating actin polymerization physical 1 1 1 Two-hybrid
BBC1 YJL020C Protein possibly involved in assembly of actin patches physical 1 1 1 Two-hybrid
CAF20 YOR276W Phosphoprotein of the mRNA cap-binding complex physical 1 1 1 Affinity Capture-RNA
CLN1 YMR199W G1 cyclin involved in regulation of the cell cycle genetic 1 1 1 Phenotypic Suppression
BIR1 YJR089W Subunit of chromosomal passenger complex (CPC) genetic 1 1 1 Positive Genetic
CLN3 YAL040C G1 cyclin involved in cell cycle progression genetic 1 1 1 Phenotypic Suppression
BFR1 YOR198C Component of mRNP complexes associated with polyribosomes physical 1 1 1 Affinity Capture-RNA
CCZ1 YBR131W Subunit of a heterodimeric guanine nucleotide exchange factor (GEF) genetic 1 1 1 Negative Genetic
CSG2 YBR036C Endoplasmic reticulum membrane protein genetic 1 1 1 Negative Genetic
CRF1 YDR223W Transcriptional corepressor physical 1 1 1 PCA
CDC50 YCR094W Endosomal protein that interacts with phospholipid flippase Drs2p physical 1 1 1 PCA
CDC50 YCR094W Endosomal protein that interacts with phospholipid flippase Drs2p genetic 1 1 1 Negative Genetic
HOF1 YMR032W F-BAR protein that regulates actin cytoskeleton organization physical 1 1 1 Two-hybrid
LSB3 YFR024C-A Protein containing a C-terminal SH3 domain physical 1 1 1 Two-hybrid
PIN3 YPR154W Negative regulator of actin nucleation-promoting factor activity physical 1 1 1 Two-hybrid
RVS167 YDR388W Calmodulin-binding actin-associated protein physical 1 1 1 Two-hybrid
YNR048W YNR048W Potential noncatalytic subunit for phospholipid translocase Dnf3p physical 1 1 1 Two-hybrid
LEM3 YNL323W Membrane protein of the plasma membrane and ER physical 1 1 1 PCA
ISW1 YBR245C ATPase subunit of imitation-switch (ISWI) class chromatin remodelers physical 1 1 1 Affinity Capture-RNA
VTS1 YOR359W Flap-structured DNA-binding and RNA-binding protein physical 1 1 1 Affinity Capture-RNA
FPK1 YNR047W Ser/Thr protein kinase physical 1 1 1 Biochemical Activity
FPK1 YNR047W Ser/Thr protein kinase genetic 1 1 1 Phenotypic Enhancement
ELF1 YKL160W Transcription elongation factor with a conserved zinc finger domain genetic 1 1 1 Negative Genetic
SCS7 YMR272C Sphingolipid alpha-hydroxylase genetic 1 1 1 Positive Genetic
PHO23 YNL097C Component of the Rpd3L histone deacetylase complex genetic 1 1 1 Negative Genetic
NPL3 YDR432W RNA-binding protein genetic 1 1 1 Synthetic Rescue
YOR1 YGR281W Plasma membrane ATP-binding cassette (ABC) transporter genetic 1 1 1 Positive Genetic
VPS27 YNR006W Endosomal protein that forms a complex with Hse1p genetic 1 1 1 Positive Genetic
PSD1 YNL169C Phosphatidylserine decarboxylase of the mitochondrial inner membrane genetic 1 1 1 Positive Genetic
GET2 YER083C Subunit of the GET complex genetic 1 1 1 Positive Genetic
ESS1 YJR017C Peptidylprolyl-cis/trans-isomerase (PPIase) genetic 1 1 1 Synthetic Growth Defect
DRS2 YAL026C Trans-golgi network aminophospholipid translocase (flippase) genetic 1 1 1 Phenotypic Enhancement
IZH2 YOL002C Plasma membrane receptor for plant antifungal osmotin genetic 1 1 1 Synthetic Growth Defect
PDX3 YBR035C Pyridoxine (pyridoxamine) phosphate oxidase genetic 1 1 1 Positive Genetic
TSC3 YBR058C-A Protein that stimulates the activity of serine palmitoyltransferase genetic 1 1 1 Positive Genetic
PRP6 YBR055C Splicing factor genetic 1 1 1 Negative Genetic
RPL3 YOR063W Ribosomal 60S subunit protein L3 genetic 1 1 1 Negative Genetic
SAC7 YDR389W GTPase activating protein (GAP) for Rho1p genetic 1 1 1 Negative Genetic
YGR237C YGR237C Putative protein of unknown function genetic 1 1 1 Negative Genetic
RIM4 YHL024W Putative RNA-binding protein genetic 1 1 1 Negative Genetic
ISC1 YER019W Inositol phosphosphingolipid phospholipase C genetic 1 1 1 Synthetic Rescue
PEX22 YAL055W Putative peroxisomal membrane protein genetic 1 1 1 Negative Genetic
UBP13 YBL067C Ubiquitin-specific protease that cleaves Ub-protein fusions genetic 1 1 1 Negative Genetic
MBA1 YBR185C Membrane-associated mitochondrial ribosome receptor genetic 1 1 1 Negative Genetic
GPR1 YDL035C Plasma membrane G protein coupled receptor (GPCR) genetic 1 1 1 Negative Genetic
SNF6 YHL025W Subunit of the SWI/SNF chromatin remodeling complex genetic 1 1 1 Negative Genetic
POT1 YIL160C 3-ketoacyl-CoA thiolase with broad chain length specificity genetic 1 1 1 Negative Genetic
PET8 YNL003C S-adenosylmethionine transporter of the mitochondrial inner membrane genetic 1 1 1 Negative Genetic
PUB1 YNL016W Poly (A)+ RNA-binding protein genetic 1 1 1 Negative Genetic
HDA1 YNL021W Catalytic subunit of class II HDA1 histone deacetylase complex genetic 1 1 1 Negative Genetic
SIW14 YNL032W Inositol phosphatase involved in inositol pyrophosphate metabolism genetic 1 1 1 Negative Genetic
LAT1 YNL071W Dihydrolipoamide acetyltransferase component (E2) of the PDC genetic 1 1 1 Negative Genetic
SWS2 YNL081C Putative mitochondrial ribosomal protein of the small subunit genetic 1 1 1 Negative Genetic
MKT1 YNL085W Protein similar to nucleases that forms a complex with Pbp1p genetic 1 1 1 Negative Genetic
YNL144C YNL144C Putative protein of unknown function genetic 1 1 1 Negative Genetic
MSN1 YOL116W Transcriptional activator genetic 1 1 1 Negative Genetic
MDM32 YOR147W Mitochondrial inner membrane protein with similarity to Mdm31p genetic 1 1 1 Negative Genetic
NBP2 YDR162C Protein involved in the HOG (high osmolarity glycerol) pathway genetic 1 1 1 Negative Genetic
HRQ1 YDR291W 3'-5' DNA helicase of the conserved RecQ family genetic 1 1 1 Negative Genetic
SGS1 YMR190C RecQ family nucleolar DNA helicase genetic 1 1 1 Negative Genetic