Standard name Systematic name Name description Description
DNF1 YER166W Aminophospholipid translocase (flippase) Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF1 has a paralog, DNF2, that arose from the whole genome duplication
Visualize the interactome of DNF1
Links to external databases:
  SGD   |   KEGG   |  

Cell cycle phase and timing of transcription peak

Cell cycle phase Time (min)

CYCLoPs localization & abundance

WT1 WT2 WT3
Bud 0.126 0.116 0.172
Cell Periphery 0.054 NaN NaN
Cort. Patches 0.354 0.440 0.413
Cytoplasm 0.111 0.138 0.083
ER 0.154 0.218 0.109
Endosome 0.071 NaN 0.053
Golgi 0.137 0.098 0.066
Mito 0.189 0.280 0.277
Peroxisome 0.074 NaN 0.215
Vac/Vac Memb 0.054 NaN NaN
Budsite NaN 0.058 NaN

GFP localization & abundance

GFP localization GFP abundance
late Golgi low signal

GO term count

Metabolism Cell cycle Cell division Signal transduction DNA replication
DNF1 0 0 0 0 0

Enzyme information

Catalyzed reactions in Yeast8

List of interactors

Use the search utility to find the interactor you are looking for. By clicking the column headers the table will be sorted on that column. Use shift+click to sort on multiple columns. Default sorting is on number of experiments, number of unique methods and the number of publications, and alphabetical on standard name, in that order.
Standard name Systematic name Name description Type #Experiments #Methods #Publications Evidence
DRS2 YAL026C Trans-golgi network aminophospholipid translocase (flippase) genetic 10 5 8 Synthetic Growth Defect, Phenotypic Enhancement, Synthetic Growth Defect, Synthetic Rescue, Dosage Rescue, Phenotypic Suppression, Phenotypic Suppression, Dosage Rescue, Phenotypic Enhancement, Phenotypic Suppression
DNF2 YDR093W Aminophospholipid translocase (flippase) genetic 9 4 9 Synthetic Lethality, Phenotypic Enhancement, Phenotypic Enhancement, Synthetic Growth Defect, Synthetic Rescue, Phenotypic Enhancement, Synthetic Growth Defect, Phenotypic Enhancement, Phenotypic Enhancement
LEM3 YNL323W Membrane protein of the plasma membrane and ER physical 7 5 6 Affinity Capture-Western, PCA, Two-hybrid, Affinity Capture-Western, PCA, Co-purification, Co-crystal Structure
CDC50 YCR094W Endosomal protein that interacts with phospholipid flippase Drs2p genetic 6 3 5 Dosage Rescue, Negative Genetic, Synthetic Growth Defect, Synthetic Growth Defect, Dosage Rescue, Negative Genetic
DNF3 YMR162C Trans-golgi network aminophospholipid translocase (flippase) genetic 3 2 3 Synthetic Rescue, Phenotypic Enhancement, Phenotypic Enhancement
CHO1 YER026C Phosphatidylserine synthase genetic 3 2 2 Dosage Rescue, Phenotypic Suppression, Dosage Rescue
FKH1 YIL131C Forkhead family transcription factor regulation 2 1 2 chromatin immunoprecipitation-chip evidence, chromatin immunoprecipitation-chip evidence
YPK1 YKL126W S/T protein kinase genetic 2 1 2 Phenotypic Suppression, Phenotypic Suppression
FAS2 YPL231W Alpha subunit of fatty acid synthetase genetic 2 2 1 Positive Genetic, Negative Genetic
MSN2 YMR037C Stress-responsive transcriptional activator regulation 1 1 1 chromatin immunoprecipitation-chip evidence
FPK1 YNR047W Ser/Thr protein kinase regulation 1 1 1 protein kinase assay evidence
CBF5 YLR175W Pseudouridine synthase catalytic subunit of box H/ACA snoRNPs genetic 1 1 1 Negative Genetic
CDC42 YLR229C Small rho-like GTPase genetic 1 1 1 Negative Genetic
CSL4 YNL232W Exosome non-catalytic core component genetic 1 1 1 Negative Genetic
CCR4 YAL021C Component of the CCR4-NOT transcriptional complex physical 1 1 1 Affinity Capture-RNA
AOS1 YPR180W Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Uba2p genetic 1 1 1 Negative Genetic
DBP10 YDL031W Putative ATP-dependent RNA helicase of the DEAD-box protein family genetic 1 1 1 Negative Genetic
DBP2 YNL112W ATP-dependent RNA helicase of the DEAD-box protein family genetic 1 1 1 Negative Genetic
CSE1 YGL238W Nuclear envelope protein that acts as a recycling factor genetic 1 1 1 Negative Genetic
CCT7 YJL111W Subunit of the cytosolic chaperonin Cct ring complex genetic 1 1 1 Negative Genetic
ATG9 YDL149W Transmembrane protein involved in forming Cvt and autophagic vesicles physical 1 1 1 Affinity Capture-MS
AAC3 YBR085W Mitochondrial inner membrane ADP/ATP translocator genetic 1 1 1 Positive Genetic
CLN1 YMR199W G1 cyclin involved in regulation of the cell cycle genetic 1 1 1 Phenotypic Suppression
ACS2 YLR153C Acetyl-coA synthetase isoform genetic 1 1 1 Negative Genetic
CDC60 YPL160W Cytosolic leucyl tRNA synthetase physical 1 1 1 Affinity Capture-MS
ADH4 YGL256W Alcohol dehydrogenase isoenzyme type IV genetic 1 1 1 Negative Genetic
AIM46 YHR199C Protein of unknown function genetic 1 1 1 Negative Genetic
CLN3 YAL040C G1 cyclin involved in cell cycle progression genetic 1 1 1 Phenotypic Suppression
ATG27 YJL178C Type I membrane protein involved in autophagy and the Cvt pathway physical 1 1 1 Affinity Capture-MS
ART5 YGR068C Protein proposed to regulate endocytosis of plasma membrane proteins genetic 1 1 1 Dosage Rescue
COG4 YPR105C Essential component of the conserved oligomeric Golgi complex genetic 1 1 1 Negative Genetic
APC11 YDL008W Catalytic core subunit, Anaphase-Promoting Complex/Cyclosome (APC/C) genetic 1 1 1 Negative Genetic
COG7 YGL005C Component of the conserved oligomeric Golgi complex genetic 1 1 1 Negative Genetic
ATG23 YLR431C Peripheral membrane protein required for autophagy and CVT physical 1 1 1 Affinity Capture-MS
ATG8 YBL078C Ub-like protein physical 1 1 1 Affinity Capture-MS
BFR1 YOR198C Component of mRNP complexes associated with polyribosomes physical 1 1 1 Affinity Capture-RNA
CHS3 YBR023C Chitin synthase III physical 1 1 1 PCA
CHS5 YLR330W Component of the exomer complex physical 1 1 1 PCA
CNN1 YFR046C Kinetochore protein genetic 1 1 1 Negative Genetic
BTS1 YPL069C Geranylgeranyl diphosphate synthase (GGPPS) genetic 1 1 1 Negative Genetic
BPH1 YCR032W Protein homologous to Chediak-Higashi syndrome and Beige proteins genetic 1 1 1 Negative Genetic
CUL3 YGR003W Ubiquitin-protein ligase genetic 1 1 1 Negative Genetic
ANY1 YMR010W Protein involved in phospholipid flippase function genetic 1 1 1 Dosage Lethality
PAP2 YOL115W Non-canonical poly(A) polymerase physical 1 1 1 Affinity Capture-MS
RCY1 YJL204C F-box protein involved in recycling endocytosed proteins physical 1 1 1 Affinity Capture-Western
DRS2 YAL026C Trans-golgi network aminophospholipid translocase (flippase) physical 1 1 1 PCA
FTH1 YBR207W Putative high affinity iron transporter physical 1 1 1 PCA
SYP1 YCR030C Negative regulator of WASP-Arp23 complex physical 1 1 1 PCA
DNF2 YDR093W Aminophospholipid translocase (flippase) physical 1 1 1 PCA
SEC7 YDR170C Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors physical 1 1 1 PCA
SEC6 YIL068C Essential 88kDa subunit of the exocyst complex physical 1 1 1 PCA
PIN2 YOR104W Exomer-dependent cargo protein physical 1 1 1 PCA
YAP1802 YGR241C Protein of the AP180 family, involved in clathrin cage assembly physical 1 1 1 PCA
LSB3 YFR024C-A Protein containing a C-terminal SH3 domain physical 1 1 1 PCA
SMI1 YGR229C Protein involved in the regulation of cell wall synthesis physical 1 1 1 PCA
DNF1 YER166W Aminophospholipid translocase (flippase) physical 1 1 1 PCA
KEX1 YGL203C Cell death protease essential for hypochlorite-induced apoptosis physical 1 1 1 PCA
TCB3 YML072C Cortical ER protein involved in ER-plasma membrane tethering physical 1 1 1 Two-hybrid
EMP24 YGL200C Component of the p24 complex physical 1 1 1 PCA
PAN1 YIR006C Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p physical 1 1 1 Affinity Capture-MS
SBP1 YHL034C Protein that binds eIF4G and has a role in repression of translation physical 1 1 1 Affinity Capture-RNA
PUF3 YLL013C Protein of the mitochondrial outer surface physical 1 1 1 Affinity Capture-RNA
VPS15 YBR097W Serine/threonine protein kinase involved in vacuolar protein sorting physical 1 1 1 Affinity Capture-MS
TOM1 YDR457W E3 ubiquitin ligase of the hect-domain class physical 1 1 1 Affinity Capture-MS
ISW1 YBR245C ATPase subunit of imitation-switch (ISWI) class chromatin remodelers physical 1 1 1 Affinity Capture-RNA
FPK1 YNR047W Ser/Thr protein kinase physical 1 1 1 Biochemical Activity
FPK1 YNR047W Ser/Thr protein kinase genetic 1 1 1 Synthetic Rescue
HRR25 YPL204W Conserved casein kinase, monopolin subunit physical 1 1 1 Affinity Capture-MS
MCA1 YOR197W Ca2+-dependent cysteine protease physical 1 1 1 Affinity Capture-MS
MES1 YGR264C Methionyl-tRNA synthetase physical 1 1 1 Affinity Capture-RNA
HSP82 YPL240C Hsp90 chaperone genetic 1 1 1 Synthetic Growth Defect
HSC82 YMR186W Cytoplasmic chaperone of the Hsp90 family genetic 1 1 1 Synthetic Growth Defect
SEC14 YMR079W Phosphatidylinositol/phosphatidylcholine transfer protein genetic 1 1 1 Synthetic Growth Defect
PDR10 YOR328W ATP-binding cassette (ABC) transporter genetic 1 1 1 Phenotypic Enhancement
KES1 YPL145C Sterol/phosphatidylinositol-4-phosphate (PI(4)P) exchanger genetic 1 1 1 Synthetic Rescue
RVS161 YCR009C Amphiphysin-like lipid raft protein genetic 1 1 1 Positive Genetic
SRO7 YPR032W Effector of Rab GTPase Sec4p genetic 1 1 1 Positive Genetic
SEC1 YDR164C Sm-like protein involved in docking and fusion of exocytic vesicles genetic 1 1 1 Negative Genetic
NEO1 YIL048W Phospholipid translocase (flippase) genetic 1 1 1 Dosage Rescue
PSD2 YGR170W Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes genetic 1 1 1 Dosage Rescue
PKC1 YBL105C Protein serine/threonine kinase genetic 1 1 1 Positive Genetic
HEM1 YDR232W 5-aminolevulinate synthase genetic 1 1 1 Positive Genetic
NOP9 YJL010C Essential subunit of U3-containing 90S preribosome genetic 1 1 1 Positive Genetic
SNG1 YGR197C Protein involved in resistance to nitrosoguanidine and 6-azauracil genetic 1 1 1 Positive Genetic
YHL008C YHL008C Putative protein of unknown function genetic 1 1 1 Positive Genetic
SSO2 YMR183C Plasma membrane t-SNARE genetic 1 1 1 Positive Genetic
SLA2 YNL243W Adaptor protein that links actin to clathrin and endocytosis genetic 1 1 1 Positive Genetic
POL30 YBR088C Proliferating cell nuclear antigen (PCNA) genetic 1 1 1 Negative Genetic
RPC53 YDL150W RNA polymerase III subunit C53 genetic 1 1 1 Negative Genetic
SAS10 YDL153C Subunit of U3-containing Small Subunit (SSU) processome complex genetic 1 1 1 Negative Genetic
ESF1 YDR365C Nucleolar protein involved in pre-rRNA processing genetic 1 1 1 Negative Genetic
RPS2 YGL123W Protein component of the small (40S) subunit genetic 1 1 1 Negative Genetic
ERG7 YHR072W Lanosterol synthase genetic 1 1 1 Negative Genetic
QNS1 YHR074W Glutamine-dependent NAD(+) synthetase genetic 1 1 1 Negative Genetic
YKT6 YKL196C Vesicle membrane protein (v-SNARE) with acyltransferase activity genetic 1 1 1 Negative Genetic
HSP60 YLR259C Tetradecameric mitochondrial chaperonin genetic 1 1 1 Negative Genetic
RRN9 YMR270C Protein involved in promoting high level transcription of rDNA genetic 1 1 1 Negative Genetic
FCP1 YMR277W Carboxy-terminal domain (CTD) phosphatase genetic 1 1 1 Negative Genetic
PBR1 YNL181W Putative oxidoreductase genetic 1 1 1 Negative Genetic
WRS1 YOL097C Cytoplasmic tryptophanyl-tRNA synthetase genetic 1 1 1 Negative Genetic
SEN54 YPL083C Subunit of the tRNA splicing endonuclease genetic 1 1 1 Negative Genetic
IDI1 YPL117C Isopentenyl diphosphate:dimethylallyl diphosphate isomerase genetic 1 1 1 Negative Genetic
NOP2 YNL061W rRNA m5C methyltransferase genetic 1 1 1 Negative Genetic
MAK21 YDR060W Constituent of 66S pre-ribosomal particles genetic 1 1 1 Negative Genetic
PRP28 YDR243C Pre-mRNA splicing factor genetic 1 1 1 Negative Genetic
MOB2 YFL034C-B Activator of Cbk1p kinase genetic 1 1 1 Negative Genetic
UTP9 YHR196W Nucleolar protein genetic 1 1 1 Negative Genetic
NUP192 YJL039C Essential subunit of inner ring of nuclear pore complex (NPC) genetic 1 1 1 Negative Genetic
PRP21 YJL203W Subunit of the SF3a splicing factor complex genetic 1 1 1 Negative Genetic
GRC3 YLL035W Polynucleotide kinase present on rDNA genetic 1 1 1 Negative Genetic
PRP19 YLL036C Splicing factor associated with the spliceosome genetic 1 1 1 Negative Genetic
SEC65 YML105C Subunit of the signal recognition particle (SRP) genetic 1 1 1 Negative Genetic
GPI12 YMR281W ER membrane protein involved in the second step of GPI anchor assembly genetic 1 1 1 Negative Genetic
SEC62 YPL094C Essential subunit of Sec63 complex genetic 1 1 1 Negative Genetic
PRE2 YPR103W Beta 5 subunit of the 20S proteasome genetic 1 1 1 Negative Genetic
RBG1 YAL036C Member of the DRG family of GTP-binding proteins genetic 1 1 1 Negative Genetic
TSC3 YBR058C-A Protein that stimulates the activity of serine palmitoyltransferase genetic 1 1 1 Negative Genetic
GDT1 YBR187W Calcium and manganese transporter with higher affinity for Ca2+ genetic 1 1 1 Negative Genetic
MIC12 YBR262C Component of the MICOS complex genetic 1 1 1 Negative Genetic
RAM1 YDL090C Beta subunit of the CAAX farnesyltransferase (FTase) genetic 1 1 1 Negative Genetic
YER039C-A YER039C-A Putative protein of unknown function genetic 1 1 1 Negative Genetic
MIL1 YFL034W Predicted lipase genetic 1 1 1 Negative Genetic
ERV14 YGL054C COPII-coated vesicle protein genetic 1 1 1 Negative Genetic
TAD1 YGL243W tRNA-specific adenosine deaminase genetic 1 1 1 Negative Genetic
YGR016W YGR016W Putative protein of unknown function genetic 1 1 1 Negative Genetic
YGR111W YGR111W Putative protein of unknown function genetic 1 1 1 Negative Genetic
YGR153W YGR153W Putative protein of unknown function genetic 1 1 1 Negative Genetic
PCL5 YHR071W Cyclin genetic 1 1 1 Negative Genetic
ESL2 YKR096W hEST1A/B (SMG5/6)-like protein genetic 1 1 1 Negative Genetic
SKG1 YKR100C Transmembrane protein with a role in cell wall polymer composition genetic 1 1 1 Negative Genetic
PML1 YLR016C Subunit of the RES complex genetic 1 1 1 Negative Genetic
YMR030W-A YMR030W-A Putative protein of unknown function genetic 1 1 1 Negative Genetic
RRG9 YNL213C Protein of unknown function genetic 1 1 1 Negative Genetic
MDM32 YOR147W Mitochondrial inner membrane protein with similarity to Mdm31p genetic 1 1 1 Negative Genetic
RUD3 YOR216C Golgi matrix protein genetic 1 1 1 Negative Genetic
MET7 YOR241W Folylpolyglutamate synthetase genetic 1 1 1 Negative Genetic
RQC2 YPL009C Component of the Ribosome Quality Control (RQC) complex genetic 1 1 1 Negative Genetic
ITR1 YDR497C Myo-inositol transporter genetic 1 1 1 Synthetic Rescue
ITR2 YOL103W Myo-inositol transporter genetic 1 1 1 Synthetic Rescue
INN1 YNL152W Essential protein that associates with contractile actomyosin ring genetic 1 1 1 Synthetic Rescue
HRQ1 YDR291W 3'-5' DNA helicase of the conserved RecQ family genetic 1 1 1 Negative Genetic
SGS1 YMR190C RecQ family nucleolar DNA helicase genetic 1 1 1 Negative Genetic
CHS6 YJL099W Member of the ChAPs (Chs5p-Arf1p-binding proteins) family genetic 1 1 1 Dosage Rescue
COQ4 YDR204W Protein with a role in ubiquinone (Coenzyme Q) biosynthesis genetic 1 1 1 Positive Genetic
COX20 YDR231C Mitochondrial inner membrane protein genetic 1 1 1 Positive Genetic