Standard name Systematic name Name description Description
PUS1 YPL212C tRNA:pseudouridine synthase tRNA:pseudouridine synthase; introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; also acts on U2 snRNA; also pseudouridylates some mRNAs, and pseudouridylation level varies with growth phase; nuclear protein that appears to be involved in tRNA export; PUS1 has a paralog, PUS2, that arose from the whole genome duplication
Visualize the interactome of PUS1
Links to external databases:
  SGD   |   KEGG   |  

Cell cycle phase and timing of transcription peak

Cell cycle phase Time (min)

CYCLoPs localization & abundance

WT1 WT2 WT3
Nucleus 0.945 0.977 0.916

GFP localization & abundance

GFP localization GFP abundance
nucleus 8130

GO term count

Metabolism Cell cycle Cell division Signal transduction DNA replication
PUS1 4 0 0 0 0

Enzyme information

Catalyzed reactions in Yeast8
r_1095 (yUMP synthetase)

List of interactors

Use the search utility to find the interactor you are looking for. By clicking the column headers the table will be sorted on that column. Use shift+click to sort on multiple columns. Default sorting is on number of experiments, number of unique methods and the number of publications, and alphabetical on standard name, in that order.
Standard name Systematic name Name description Type #Experiments #Methods #Publications Evidence
MAF1 YDR005C Highly conserved negative regulator of RNA polymerase III genetic 5 2 4 Synthetic Growth Defect, Synthetic Growth Defect, Negative Genetic, Negative Genetic, Negative Genetic
LOS1 YKL205W Nuclear pore protein genetic 4 3 4 Synthetic Lethality, Synthetic Growth Defect, Negative Genetic, Synthetic Growth Defect
TRM82 YDR165W Noncatalytic subunit of a tRNA methyltransferase complex genetic 4 2 4 Synthetic Growth Defect, Negative Genetic, Negative Genetic, Negative Genetic
TRM8 YDL201W Catalytic subunit of a tRNA methyltransferase complex genetic 4 2 3 Synthetic Growth Defect, Negative Genetic, Negative Genetic, Negative Genetic
TRM10 YOL093W tRNA methyltransferase genetic 4 2 3 Synthetic Growth Defect, Negative Genetic, Negative Genetic, Negative Genetic
GCN2 YDR283C Protein kinase genetic 3 1 3 Negative Genetic, Negative Genetic, Negative Genetic
SSB2 YNL209W Cytoplasmic ATPase that is a ribosome-associated molecular chaperone physical 3 2 2 Affinity Capture-MS, Affinity Capture-MS, Co-purification
DUS3 YLR401C Dihydrouridine synthase genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
FKH1 YIL131C Forkhead family transcription factor regulation 2 2 2 chromatin immunoprecipitation-chip evidence, ChEC-Seq experiments
GCN20 YFR009W Positive regulator of the Gcn2p kinase activity genetic 2 2 2 Phenotypic Enhancement, Negative Genetic
HIR2 YOR038C Subunit of HIR nucleosome assembly complex genetic 2 2 2 Phenotypic Suppression, Positive Genetic
PUS4 YNL292W Pseudouridine synthase genetic 2 1 2 Negative Genetic, Negative Genetic
GCN1 YGL195W Positive regulator of the Gcn2p kinase activity genetic 2 1 2 Negative Genetic, Negative Genetic
DHH1 YDL160C Cytoplasmic DEAD-box helicase and mRNA decapping activator physical 2 1 2 Affinity Capture-RNA, Affinity Capture-RNA
DPH5 YLR172C Methyltransferase required for synthesis of diphthamide genetic 2 1 2 Negative Genetic, Negative Genetic
MET22 YOL064C Bisphosphate-3'-nucleotidase genetic 2 2 1 Synthetic Rescue, Phenotypic Suppression
SSB1 YDL229W Cytoplasmic ATPase that is a ribosome-associated molecular chaperone physical 2 1 1 Affinity Capture-MS, Affinity Capture-MS
REB1 YBR049C RNA polymerase I enhancer binding protein regulation 1 1 1 chromatin immunoprecipitation-chip evidence
SUA7 YPR086W Transcription factor TFIIB regulation 1 1 1 chromatin immunoprecipitation-chip evidence
INO4 YOL108C Transcription factor involved in phospholipid synthesis regulation 1 1 1 chromatin immunoprecipitation-chip evidence
RAD53 YPL153C DNA damage response kinase regulation 1 1 1 protein kinase assay evidence
CHD1 YER164W Chromatin remodeler that regulates various aspects of transcription genetic 1 1 1 Positive Genetic
DED81 YHR019C Cytosolic asparaginyl-tRNA synthetase genetic 1 1 1 Negative Genetic
POP8 YBL018C Subunit of both RNase MRP and nuclear RNase P genetic 1 1 1 Positive Genetic
ARC1 YGL105W Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases genetic 1 1 1 Negative Genetic
CBC2 YPL178W Small subunit of the heterodimeric cap binding complex with Sto1p genetic 1 1 1 Negative Genetic
GCD11 YER025W Gamma subunit of the translation initiation factor eIF2 genetic 1 1 1 Positive Genetic
ACT1 YFL039C Actin, structural protein in cytoskeleton genetic 1 1 1 Negative Genetic
CCR4 YAL021C Component of the CCR4-NOT transcriptional complex physical 1 1 1 Affinity Capture-RNA
NAB2 YGL122C Nuclear polyadenylated RNA-binding protein physical 1 1 1 Affinity Capture-RNA
NAM7 YMR080C ATP-dependent RNA helicase of the SFI superfamily physical 1 1 1 Affinity Capture-RNA
PUS1 YPL212C tRNA:pseudouridine synthase physical 1 1 1 Affinity Capture-MS
PUS1 YPL212C tRNA:pseudouridine synthase genetic 1 1 1 Synthetic Lethality
UTP22 YGR090W Component of the small-subunit processome physical 1 1 1 Affinity Capture-MS
TAN1 YGL232W Putative tRNA acetyltransferase physical 1 1 1 Affinity Capture-MS
SBP1 YHL034C Protein that binds eIF4G and has a role in repression of translation physical 1 1 1 Affinity Capture-RNA
ULP1 YPL020C Protease that specifically cleaves Smt3p protein conjugates physical 1 1 1 PCA
RVB1 YDR190C ATP-dependent DNA helicase, also known as pontin physical 1 1 1 Affinity Capture-MS
RPC82 YPR190C RNA polymerase III subunit C82 physical 1 1 1 Affinity Capture-MS
VTS1 YOR359W Flap-structured DNA-binding and RNA-binding protein physical 1 1 1 Affinity Capture-RNA
RRP6 YOR001W Nuclear exosome exonuclease component physical 1 1 1 Affinity Capture-MS
SPO22 YIL073C Meiosis-specific protein essential for chromosome synapsis physical 1 1 1 Affinity Capture-MS
RAD53 YPL153C DNA damage response kinase physical 1 1 1 Biochemical Activity
TRM1 YDR120C tRNA methyltransferase genetic 1 1 1 Synthetic Growth Defect
YME2 YMR302C Integral inner mitochondrial membrane protein genetic 1 1 1 Positive Genetic
PUS2 YGL063W Mitochondrial tRNA:pseudouridine synthase genetic 1 1 1 Synthetic Growth Defect
SEC26 YDR238C Essential beta-coat protein of the COPI coatomer genetic 1 1 1 Positive Genetic
RSM24 YDR175C Mitochondrial ribosomal protein of the small subunit genetic 1 1 1 Positive Genetic
SLA2 YNL243W Adaptor protein that links actin to clathrin and endocytosis genetic 1 1 1 Positive Genetic
TFC6 YDR362C Subunit of RNA polymerase III transcription initiation factor complex genetic 1 1 1 Negative Genetic
SPB4 YFL002C Putative ATP-dependent RNA helicase genetic 1 1 1 Negative Genetic
UTP8 YGR128C Nucleolar protein required for export of tRNAs from the nucleus genetic 1 1 1 Negative Genetic
RLP24 YLR009W Essential protein required for ribosomal large subunit biogenesis genetic 1 1 1 Negative Genetic
RPL10 YLR075W Ribosomal 60S subunit protein L10 genetic 1 1 1 Negative Genetic
RPC19 YNL113W RNA polymerase subunit AC19 genetic 1 1 1 Negative Genetic
TEF2 YBR118W Translational elongation factor EF-1 alpha genetic 1 1 1 Negative Genetic
SEC66 YBR171W Non-essential subunit of Sec63 complex genetic 1 1 1 Negative Genetic
RAM1 YDL090C Beta subunit of the CAAX farnesyltransferase (FTase) genetic 1 1 1 Negative Genetic
SED1 YDR077W Major stress-induced structural GPI-cell wall glycoprotein genetic 1 1 1 Negative Genetic
VRP1 YLR337C Verprolin, proline-rich actin-associated protein genetic 1 1 1 Negative Genetic
RPL20B YOR312C Ribosomal 60S subunit protein L20B genetic 1 1 1 Negative Genetic
STE50 YCL032W Adaptor protein for various signaling pathways genetic 1 1 1 Negative Genetic
SRF1 YDL133W Regulator of phospholipase D (Spo14p) genetic 1 1 1 Negative Genetic
RPA14 YDR156W RNA polymerase I subunit A14 genetic 1 1 1 Negative Genetic
RPL11B YGR085C Ribosomal 60S subunit protein L11B genetic 1 1 1 Negative Genetic
TRS85 YDR108W Component of transport protein particle (TRAPP) complex III genetic 1 1 1 Negative Genetic
SWT1 YOR166C RNA endoribonuclease involved in perinuclear mRNP quality control genetic 1 1 1 Positive Genetic
SUM1 YDR310C Transcriptional repressor that regulates middle-sporulation genes genetic 1 1 1 Negative Genetic
SEC27 YGL137W Essential beta'-coat protein of the COPI coatomer genetic 1 1 1 Negative Genetic
RIM20 YOR275C Protein involved in proteolytic activation of Rim101p genetic 1 1 1 Positive Genetic
APL5 YPL195W Delta adaptin-like subunit of the clathrin associated protein complex physical 1 1 1 Affinity Capture-MS
LSM7 YNL147W Lsm (Like Sm) protein genetic 1 1 1 Negative Genetic
CMR1 YDL156W Nuclear protein with role in transcription, protein quality control genetic 1 1 1 Negative Genetic
INO80 YGL150C Nucleosome spacing factor genetic 1 1 1 Negative Genetic
NOT3 YIL038C Component of the CCR4-NOT core complex, involved in mRNA decapping genetic 1 1 1 Negative Genetic
NSP1 YJL041W FG-nucleoporin component of central core of the nuclear pore complex genetic 1 1 1 Synthetic Lethality
NUP116 YMR047C FG-nucleoporin component of central core of the nuclear pore complex genetic 1 1 1 Positive Genetic
GIM5 YML094W Subunit of the heterohexameric cochaperone prefoldin complex genetic 1 1 1 Negative Genetic
MED8 YBR193C Subunit of the RNA polymerase II mediator complex genetic 1 1 1 Negative Genetic
CSI1 YMR025W Subunit of the Cop9 signalosome genetic 1 1 1 Positive Genetic
PRP11 YDL043C Subunit of the SF3a splicing factor complex genetic 1 1 1 Negative Genetic
LSM12 YHR121W Protein of unknown function that may function in RNA processing genetic 1 1 1 Negative Genetic
HYP2 YEL034W Translation elongation factor eIF-5A genetic 1 1 1 Negative Genetic
DUN1 YDL101C Cell-cycle checkpoint S/T protein kinase genetic 1 1 1 Dosage Lethality
NUS1 YDL193W Forms dehydrodolichyl diphosphate synthase complex with RER2 or SRT1 genetic 1 1 1 Negative Genetic
KIN28 YDL108W Ser/Thr protein kinase and subunit of TFIIK, a TFIIH subassembly genetic 1 1 1 Negative Genetic
PAP2 YOL115W Non-canonical poly(A) polymerase genetic 1 1 1 Negative Genetic
BIR1 YJR089W Subunit of chromosomal passenger complex (CPC) genetic 1 1 1 Positive Genetic
DHR2 YKL078W Predominantly nucleolar DEAH-box ATP-dependent RNA helicase genetic 1 1 1 Positive Genetic
ITT1 YML068W Protein that modulates the efficiency of translation termination genetic 1 1 1 Positive Genetic
MDY2 YOL111C Protein involved in inserting tail-anchored proteins into ER membranes genetic 1 1 1 Negative Genetic
MEX67 YPL169C Poly(A)RNA binding protein involved in nuclear mRNA export genetic 1 1 1 Negative Genetic
PAN1 YIR006C Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p genetic 1 1 1 Positive Genetic
KTI11 YBL071W-A Zn-ribbon protein that co-purifies with Dph1 and Dph2 genetic 1 1 1 Negative Genetic
PRP24 YMR268C Splicing factor that reanneals snRNPs during spliceosome recycling genetic 1 1 1 Negative Genetic
FRA1 YLL029W Protein involved in negative regulation of iron regulon transcription genetic 1 1 1 Negative Genetic
PAH1 YMR165C Mg2+-dependent phosphatidate (PA) phosphatase genetic 1 1 1 Negative Genetic
HRQ1 YDR291W 3'-5' DNA helicase of the conserved RecQ family physical 1 1 1 Affinity Capture-MS
COQ11 YLR290C Putative oxidoreductase, subunit of Coenzyme Q biosynthetic complexes genetic 1 1 1 Negative Genetic
MTC1 YJL123C Protein of unknown function that may interact with ribosomes genetic 1 1 1 Negative Genetic
NOP19 YGR251W Ribosome biogenesis factor genetic 1 1 1 Negative Genetic
ORC4 YPR162C Subunit of the origin recognition complex (ORC) genetic 1 1 1 Negative Genetic
CGI121 YML036W Component of the EKC/KEOPS complex genetic 1 1 1 Negative Genetic
KAE1 YKR038C Highly conserved ATPase of HSP70/DnaK family genetic 1 1 1 Synthetic Growth Defect
APJ1 YNL077W Hsp40 chaperone with a role in SUMO-mediated protein degradation physical 1 1 1 Affinity Capture-MS
GPI13 YLL031C ER membrane localized phosphoryltransferase genetic 1 1 1 Positive Genetic
MSN1 YOL116W Transcriptional activator genetic 1 1 1 Positive Genetic
GPI10 YGL142C Integral membrane protein involved in GPI anchor synthesis genetic 1 1 1 Negative Genetic
EFM4 YIL064W Lysine methyltransferase genetic 1 1 1 Negative Genetic
PCC1 YKR095W-A Component of the EKC/KEOPS protein complex genetic 1 1 1 Synthetic Growth Defect
KRE5 YOR336W Protein required for beta-1,6 glucan biosynthesis genetic 1 1 1 Positive Genetic