Standard name Systematic name Name description Description
STE5 YDR103W Pheromone-responsive MAPK scaffold protein Pheromone-responsive MAPK scaffold protein; couples activation of the G-protein-coupled pheromone receptor to MAPK activation; intramolecular interaction of PH and VWA domains blocks activation of assembled signaling cascade components (Ste11p, Ste7p and Fus3p) under basal conditions; Gbeta-gamma (Ste4p-Ste18p)-dependent docking at the plasma membrane and binding of PI(4,5)P2 by the PH domain relieves autoinhibition, resulting in pheromone-dependent pathway activation
Visualize the interactome of STE5
Links to external databases:
  SGD   |   KEGG   |  

Cell cycle phase and timing of transcription peak

Cell cycle phase Time (min)

CYCLoPs localization & abundance

WT1 WT2 WT3
Cytoplasm 0.87 NaN 0.960
Mito 0.13 NaN 0.053

GFP localization & abundance

GFP localization GFP abundance
cytoplasm, nucleus 1900

GO term count

Metabolism Cell cycle Cell division Signal transduction DNA replication
STE5 1 0 0 2 0

List of interactors

Use the search utility to find the interactor you are looking for. By clicking the column headers the table will be sorted on that column. Use shift+click to sort on multiple columns. Default sorting is on number of experiments, number of unique methods and the number of publications, and alphabetical on standard name, in that order.
Standard name Systematic name Name description Type #Experiments #Methods #Publications Evidence
FUS3 YBL016W Mitogen-activated serine/threonine protein kinase involved in mating physical 22 7 20 Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex, Reconstituted Complex, Two-hybrid, Two-hybrid, Two-hybrid, Two-hybrid, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Two-hybrid, Affinity Capture-Western, Reconstituted Complex, Biochemical Activity, Biochemical Activity, Co-crystal Structure, FRET, FRET, Reconstituted Complex, FRET, Two-hybrid
STE7 YDL159W Signal transducing MAP kinase kinase physical 21 7 17 Affinity Capture-MS, Affinity Capture-Western, Two-hybrid, Two-hybrid, Two-hybrid, Two-hybrid, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Two-hybrid, Two-hybrid, Affinity Capture-Western, Affinity Capture-Western, FRET, FRET, Reconstituted Complex, Far Western, PCA, Reconstituted Complex, Two-hybrid, Two-hybrid
STE11 YLR362W Signal transducing MEK kinase physical 21 7 16 Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Two-hybrid, Two-hybrid, Two-hybrid, Two-hybrid, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Two-hybrid, Two-hybrid, Affinity Capture-Western, Two-hybrid, Co-fractionation, FRET, FRET, PCA, Co-purification, Two-hybrid, Two-hybrid
STE4 YOR212W G protein beta subunit physical 11 4 9 Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Two-hybrid, Two-hybrid, Two-hybrid, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex
STE5 YDR103W Pheromone-responsive MAPK scaffold protein physical 8 4 7 Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Two-hybrid, Reconstituted Complex, Two-hybrid, Affinity Capture-Western, Affinity Capture-Western
STE4 YOR212W G protein beta subunit genetic 8 5 6 Dosage Rescue, Phenotypic Enhancement, Phenotypic Enhancement, Synthetic Rescue, Synthetic Rescue, Synthetic Lethality, Phenotypic Suppression, Phenotypic Suppression
STE11 YLR362W Signal transducing MEK kinase genetic 4 3 3 Phenotypic Enhancement, Synthetic Rescue, Phenotypic Suppression, Phenotypic Enhancement
CLN2 YPL256C G1 cyclin involved in regulation of the cell cycle physical 4 1 3 Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western
BEM1 YBR200W Protein containing SH3-domains physical 3 2 3 Affinity Capture-Western, Two-hybrid, Affinity Capture-Western
KSS1 YGR040W Mitogen-activated protein kinase (MAPK) physical 3 2 3 Reconstituted Complex, Reconstituted Complex, Two-hybrid
FAR1 YJL157C CDK inhibitor and nuclear anchor genetic 3 3 2 Dosage Rescue, Phenotypic Suppression, Phenotypic Enhancement
STE50 YCL032W Adaptor protein for various signaling pathways physical 3 1 2 Two-hybrid, Two-hybrid, Two-hybrid
CDC24 YAL041W Guanine nucleotide exchange factor (GEF) for Cdc42p physical 3 2 1 Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex
STE18 YJR086W G protein gamma subunit genetic 2 2 2 Dosage Rescue, Synthetic Rescue
STE5 YDR103W Pheromone-responsive MAPK scaffold protein genetic 2 2 2 Synthetic Rescue, Phenotypic Suppression
STE7 YDL159W Signal transducing MAP kinase kinase genetic 2 2 2 Phenotypic Enhancement, Phenotypic Suppression
STE2 YFL026W Receptor for alpha-factor pheromone genetic 2 2 2 Dosage Rescue, Phenotypic Suppression
CDC39 YCR093W Subunit of the CCR4-NOT1 core complex genetic 2 1 2 Synthetic Rescue, Synthetic Rescue
FUS3 YBL016W Mitogen-activated serine/threonine protein kinase involved in mating genetic 2 1 2 Phenotypic Suppression, Phenotypic Suppression
STE20 YHL007C Cdc42p-activated signal transducing kinase genetic 2 1 2 Dosage Rescue, Dosage Rescue
PKC1 YBL105C Protein serine/threonine kinase regulation 2 2 1 physical interaction evidence, protein kinase assay evidence
PKC1 YBL105C Protein serine/threonine kinase physical 2 2 1 Biochemical Activity, PCA
CDC28 YBR160W Cyclin-dependent kinase (CDK) catalytic subunit regulation 1 1 1 quantitative mass spectrometry evidence
SIR2 YDL042C Conserved NAD+ dependent histone deacetylase of the Sirtuin family regulation 1 1 1 RNA-sequencing evidence
SIR3 YLR442C Silencing protein regulation 1 1 1 RNA-sequencing evidence
SIR4 YDR227W SIR protein involved in assembly of silent chromatin domains regulation 1 1 1 RNA-sequencing evidence
SRB5 YGR104C Subunit of the RNA polymerase II mediator complex regulation 1 1 1 chromatin immunoprecipitation-chip evidence
RVB2 YPL235W ATP-dependent DNA helicase, also known as reptin physical 1 1 1 Affinity Capture-MS
CCR4 YAL021C Component of the CCR4-NOT transcriptional complex physical 1 1 1 Affinity Capture-RNA
CDC28 YBR160W Cyclin-dependent kinase (CDK) catalytic subunit genetic 1 1 1 Dosage Lethality
CDC36 YDL165W Component of the CCR4-NOT core complex, involved in mRNA decapping genetic 1 1 1 Synthetic Rescue
DHH1 YDL160C Cytoplasmic DEAD-box helicase and mRNA decapping activator physical 1 1 1 Affinity Capture-RNA
ISW1 YBR245C ATPase subunit of imitation-switch (ISWI) class chromatin remodelers physical 1 1 1 Affinity Capture-RNA
KGD2 YDR148C Dihydrolipoyl transsuccinylase physical 1 1 1 Affinity Capture-MS
CMR1 YDL156W Nuclear protein with role in transcription, protein quality control physical 1 1 1 Affinity Capture-MS
MYO2 YOR326W Type V myosin motor involved in actin-based transport of cargos physical 1 1 1 Affinity Capture-Western
HSP82 YPL240C Hsp90 chaperone genetic 1 1 1 Phenotypic Suppression
CDC25 YLR310C Membrane bound guanine nucleotide exchange factor genetic 1 1 1 Dosage Rescue
PTC1 YDL006W Type 2C protein phosphatase (PP2C) physical 1 1 1 Reconstituted Complex
HEK2 YBL032W RNA binding protein involved in asymmetric localization of ASH1 mRNA physical 1 1 1 Affinity Capture-RNA
CLN1 YMR199W G1 cyclin involved in regulation of the cell cycle physical 1 1 1 Affinity Capture-Western
CBK1 YNL161W Serine/threonine protein kinase of the the RAM signaling network physical 1 1 1 Two-hybrid
ELM1 YKL048C Serine/threonine protein kinase genetic 1 1 1 Phenotypic Suppression
CDC4 YFL009W F-box protein required for both the G1/S and G2/M phase transitions physical 1 1 1 Affinity Capture-MS
HOG1 YLR113W Mitogen-activated protein kinase involved in osmoregulation genetic 1 1 1 Phenotypic Suppression
STE12 YHR084W Transcription factor that is activated by a MAPK signaling cascade genetic 1 1 1 Dosage Rescue
HHT1 YBR010W Histone H3 physical 1 1 1 Affinity Capture-MS
SLT2 YHR030C Serine/threonine MAP kinase physical 1 1 1 PCA
CLB2 YPR119W B-type cyclin involved in cell cycle progression physical 1 1 1 Affinity Capture-Western
MSG5 YNL053W Dual-specificity protein phosphatase genetic 1 1 1 Phenotypic Enhancement
GPA1 YHR005C Subunit of G protein involved in pheromone response genetic 1 1 1 Synthetic Rescue
PMD1 YER132C Protein with an N-terminal kelch-like domain physical 1 1 1 Two-hybrid
CLN3 YAL040C G1 cyclin involved in cell cycle progression genetic 1 1 1 Dosage Rescue
GIP1 YBR045C Meiosis-specific regulatory subunit of the Glc7p protein phosphatase physical 1 1 1 Two-hybrid
MSN5 YDR335W Karyopherin physical 1 1 1 Affinity Capture-Western
STE18 YJR086W G protein gamma subunit physical 1 1 1 Affinity Capture-Western
SKO1 YNL167C Basic leucine zipper transcription factor of the ATF/CREB family genetic 1 1 1 Phenotypic Suppression
STT4 YLR305C Phosphatidylinositol-4-kinase physical 1 1 1 Co-localization
YEL023C YEL023C Putative protein of unknown function physical 1 1 1 Affinity Capture-MS
VAM6 YDL077C Guanine nucleotide exchange factor for the GTPase Gtr1p physical 1 1 1 Affinity Capture-MS
VTS1 YOR359W Flap-structured DNA-binding and RNA-binding protein physical 1 1 1 Affinity Capture-RNA
CLB5 YPR120C B-type cyclin involved in DNA replication during S phase physical 1 1 1 Affinity Capture-Western
AKR1 YDR264C Palmitoyl transferase involved in protein palmitoylation genetic 1 1 1 Synthetic Rescue
SST2 YLR452C GTPase-activating protein for Gpa1p genetic 1 1 1 Phenotypic Enhancement
RSR1 YGR152C Ras-type GTPase genetic 1 1 1 Phenotypic Enhancement
FUS1 YCL027W Membrane protein localized to the shmoo tip physical 1 1 1 Protein-peptide
HHT2 YNL031C Histone H3 physical 1 1 1 Affinity Capture-MS
PMT4 YJR143C Protein O-mannosyltransferase genetic 1 1 1 Phenotypic Enhancement
SSB2 YNL209W Cytoplasmic ATPase that is a ribosome-associated molecular chaperone physical 1 1 1 Co-purification
OKP1 YGR179C Outer kinetochore protein required for accurate chromosome segregation physical 1 1 1 Two-hybrid
ARV1 YLR242C Cortical ER protein genetic 1 1 1 Phenotypic Suppression
CRD1 YDL142C Cardiolipin synthase genetic 1 1 1 Synthetic Lethality