Standard name Systematic name Name description Description
RPN3 YER021W Essential non-ATPase regulatory subunit of the 26S proteasome lid Essential non-ATPase regulatory subunit of the 26S proteasome lid; similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
Visualize the interactome of RPN3
Links to external databases:
  SGD   |   KEGG   |  

Cell cycle phase and timing of transcription peak

Cell cycle phase Time (min)

CYCLoPs localization & abundance

WT1 WT2 WT3
Cytoplasm 0.96 0.985 0.982

GFP localization & abundance

GFP localization GFP abundance
cytoplasm, nucleus 16700

GO term count

Metabolism Cell cycle Cell division Signal transduction DNA replication
RPN3 2 0 0 0 0

List of interactors

Use the search utility to find the interactor you are looking for. By clicking the column headers the table will be sorted on that column. Use shift+click to sort on multiple columns. Default sorting is on number of experiments, number of unique methods and the number of publications, and alphabetical on standard name, in that order.
Standard name Systematic name Name description Type #Experiments #Methods #Publications Evidence
RPN11 YFR004W Metalloprotease subunit of 19S regulatory particle physical 23 4 22 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Reconstituted Complex, Affinity Capture-MS, Reconstituted Complex, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-Western, Co-purification, Affinity Capture-MS, Co-purification, Affinity Capture-MS, Reconstituted Complex, Co-purification
RPN7 YPR108W Essential non-ATPase regulatory subunit of the 26S proteasome physical 9 3 9 Two-hybrid, Affinity Capture-MS, Affinity Capture-MS, Two-hybrid, Two-hybrid, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western
RPN6 YDL097C Essential, non-ATPase regulatory subunit of the 26S proteasome lid physical 8 4 8 Affinity Capture-MS, Co-purification, Co-purification, Affinity Capture-MS, Affinity Capture-MS, Reconstituted Complex, Affinity Capture-Western, Affinity Capture-Western
RPN5 YDL147W Subunit of the CSN and 26S proteasome lid complexes physical 8 3 8 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Co-purification, PCA
RPN1 YHR027C Non-ATPase base subunit of the 19S RP of the 26S proteasome physical 8 4 7 Affinity Capture-MS, Co-purification, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-MS, Affinity Capture-MS
RPN12 YFR052W Subunit of the 19S regulatory particle of the 26S proteasome lid physical 6 3 6 Affinity Capture-MS, Two-hybrid, Two-hybrid, Affinity Capture-MS, Affinity Capture-MS, Reconstituted Complex
SEM1 YDR363W-A 19S proteasome regulatory particle lid subcomplex component physical 5 2 5 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western
RPN10 YHR200W Proteasome polyubiquitin receptor physical 5 1 5 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
PRE1 YER012W Beta 4 subunit of the 20S proteasome physical 4 2 4 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western
NAS6 YGR232W Evolutionarily conserved 19S regulatory particle assembly-chaperone physical 4 2 4 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western
RPN12 YFR052W Subunit of the 19S regulatory particle of the 26S proteasome lid genetic 3 2 3 Synthetic Lethality, Dosage Rescue, Dosage Rescue
RPN8 YOR261C Essential non-ATPase regulatory subunit of the 26S proteasome physical 3 2 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western
RPT3 YDR394W ATPase of the 19S regulatory particle of the 26S proteasome physical 3 2 3 Affinity Capture-MS, Co-purification, Affinity Capture-MS
HSM3 YBR272C Evolutionarily conserved 19S regulatory particle assembly-chaperone physical 3 2 3 Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-MS
RPN2 YIL075C Subunit of the 26S proteasome physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
RPT1 YKL145W ATPase of the 19S regulatory particle of the 26S proteasome physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
UBP6 YFR010W Ubiquitin-specific protease physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
ECM29 YHL030W Scaffold protein physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
PRE9 YGR135W Alpha 3 subunit of the 20S proteasome genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
RAD23 YEL037C Protein with ubiquitin-like N terminus physical 2 2 2 Affinity Capture-MS, Reconstituted Complex
RPN14 YGL004C Evolutionarily conserved 19S regulatory particle assembly-chaperone physical 2 2 2 Affinity Capture-MS, Affinity Capture-Western
RPN9 YDR427W Non-ATPase regulatory subunit of the 26S proteasome physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
RPT5 YOR117W ATPase of the 19S regulatory particle of the 26S proteasome physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
CDC28 YBR160W Cyclin-dependent kinase (CDK) catalytic subunit physical 2 2 1 Affinity Capture-Western, Co-purification
HSC82 YMR186W Cytoplasmic chaperone of the Hsp90 family genetic 2 2 1 Synthetic Growth Defect, Dosage Rescue
MEC1 YBR136W Genome integrity checkpoint protein and PI kinase superfamily member genetic 2 2 1 Synthetic Rescue, Synthetic Growth Defect
RPN10 YHR200W Proteasome polyubiquitin receptor genetic 2 1 1 Negative Genetic, Negative Genetic
RPN11 YFR004W Metalloprotease subunit of 19S regulatory particle genetic 2 1 1 Negative Genetic, Negative Genetic
RPN2 YIL075C Subunit of the 26S proteasome genetic 2 1 1 Negative Genetic, Negative Genetic
RPN8 YOR261C Essential non-ATPase regulatory subunit of the 26S proteasome genetic 2 1 1 Negative Genetic, Negative Genetic
RPT2 YDL007W ATPase of the 19S regulatory particle of the 26S proteasome genetic 2 1 1 Negative Genetic, Negative Genetic
SSE1 YPL106C ATPase component of heat shock protein Hsp90 chaperone complex physical 2 1 1 Affinity Capture-MS, Affinity Capture-MS
SCL1 YGL011C Alpha 1 subunit of the 20S proteasome genetic 2 1 1 Negative Genetic, Negative Genetic
ACT1 YFL039C Actin, structural protein in cytoskeleton genetic 2 1 1 Synthetic Haploinsufficiency, Synthetic Haploinsufficiency
PRE10 YOR362C Alpha 7 subunit of the 20S proteasome genetic 2 1 1 Negative Genetic, Negative Genetic
PRE2 YPR103W Beta 5 subunit of the 20S proteasome genetic 2 1 1 Positive Genetic, Positive Genetic
PRE5 YMR314W Alpha 6 subunit of the 20S proteasome genetic 2 1 1 Negative Genetic, Negative Genetic
PRE6 YOL038W Alpha 4 subunit of the 20S proteasome genetic 2 1 1 Negative Genetic, Negative Genetic
PRE8 YML092C Alpha 2 subunit of the 20S proteasome genetic 2 1 1 Negative Genetic, Negative Genetic
PRE4 YFR050C Beta 7 subunit of the 20S proteasome genetic 2 1 1 Negative Genetic, Negative Genetic
PUP1 YOR157C Beta 2 subunit of the 20S proteasome genetic 2 1 1 Negative Genetic, Negative Genetic
PUP2 YGR253C Alpha 5 subunit of the 20S proteasome genetic 2 1 1 Negative Genetic, Negative Genetic
IXR1 YKL032C Transcriptional repressor that regulates hypoxic genes during normoxia regulation 1 1 1 DNA to cDNA expression microarray evidence
FKH2 YNL068C Forkhead family transcription factor regulation 1 1 1 chromatin immunoprecipitation-chip evidence
MED2 YDL005C Subunit of the RNA polymerase II mediator complex regulation 1 1 1 chromatin immunoprecipitation-chip evidence
RPN4 YDL020C Transcription factor that stimulates expression of proteasome genes regulation 1 1 1 chromatin immunoprecipitation-chip evidence
YAP6 YDR259C Basic leucine zipper (bZIP) transcription factor regulation 1 1 1 chromatin immunoprecipitation-chip evidence
REB1 YBR049C RNA polymerase I enhancer binding protein regulation 1 1 1 chromatin immunoprecipitation-chip evidence
RPD3 YNL330C Histone deacetylase, component of both the Rpd3S and Rpd3L complexes regulation 1 1 1 chromatin immunoprecipitation-chip evidence
INO2 YDR123C Transcription factor regulation 1 1 1 chromatin immunoprecipitation-chip evidence
ASH1 YKL185W Component of the Rpd3L histone deacetylase complex regulation 1 1 1 chromatin immunoprecipitation-chip evidence
CDC73 YLR418C Component of the Paf1p complex genetic 1 1 1 Synthetic Lethality
ESA1 YOR244W Catalytic subunit of the histone acetyltransferase complex (NuA4) physical 1 1 1 Affinity Capture-MS
NOP1 YDL014W Histone glutamine methyltransferase, modifies H2A at Q105 in nucleolus physical 1 1 1 Affinity Capture-MS
RPC40 YPR110C RNA polymerase subunit AC40 physical 1 1 1 Affinity Capture-MS
RET1 YOR207C Second-largest subunit of RNA polymerase III physical 1 1 1 Affinity Capture-MS
RPC82 YPR190C RNA polymerase III subunit C82 physical 1 1 1 Affinity Capture-MS
SEM1 YDR363W-A 19S proteasome regulatory particle lid subcomplex component genetic 1 1 1 Dosage Rescue
RPT2 YDL007W ATPase of the 19S regulatory particle of the 26S proteasome physical 1 1 1 Affinity Capture-MS
RPT6 YGL048C ATPase of the 19S regulatory particle of the 26S proteasome physical 1 1 1 Affinity Capture-MS
YDL199C YDL199C Putative transporter physical 1 1 1 Affinity Capture-MS
SDD1 YEL057C Protein of unknown function physical 1 1 1 Affinity Capture-MS
VPS45 YGL095C Protein of the Sec1p/Munc-18 family physical 1 1 1 Affinity Capture-MS
THR1 YHR025W Homoserine kinase physical 1 1 1 Affinity Capture-MS
STE12 YHR084W Transcription factor that is activated by a MAPK signaling cascade physical 1 1 1 Affinity Capture-MS
TDH2 YJR009C Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 2 physical 1 1 1 Affinity Capture-MS
YPF1 YKL100C Intramembrane aspartyl protease of the perinuclear ER membrane physical 1 1 1 Affinity Capture-MS
SIR1 YKR101W Protein involved in silencing at mating-type loci HML and HMR physical 1 1 1 Affinity Capture-MS
VTA1 YLR181C Multivesicular body (MVB) protein physical 1 1 1 Affinity Capture-MS
TLG2 YOL018C Syntaxin-like t-SNARE physical 1 1 1 Affinity Capture-MS
RPT4 YOR259C ATPase of the 19S regulatory particle of the 26S proteasome physical 1 1 1 Affinity Capture-MS
SNF8 YPL002C Component of the ESCRT-II complex physical 1 1 1 Affinity Capture-MS
YPK1 YKL126W S/T protein kinase physical 1 1 1 PCA
UBI4 YLL039C Ubiquitin physical 1 1 1 Affinity Capture-Western
SEC18 YBR080C AAA ATPase and SNARE disassembly chaperone physical 1 1 1 Affinity Capture-Western
SBP1 YHL034C Protein that binds eIF4G and has a role in repression of translation physical 1 1 1 Affinity Capture-RNA
SIZ1 YDR409W SUMO E3 ligase physical 1 1 1 Affinity Capture-MS
SPT21 YMR179W Protein with a role in transcriptional silencing physical 1 1 1 Affinity Capture-MS
SSB2 YNL209W Cytoplasmic ATPase that is a ribosome-associated molecular chaperone physical 1 1 1 Co-purification
RVB2 YPL235W ATP-dependent DNA helicase, also known as reptin physical 1 1 1 Affinity Capture-MS
RVB1 YDR190C ATP-dependent DNA helicase, also known as pontin physical 1 1 1 Affinity Capture-MS
TIF4631 YGR162W Translation initiation factor eIF4G and scaffold protein physical 1 1 1 Affinity Capture-MS
UTP10 YJL109C Nucleolar protein physical 1 1 1 Affinity Capture-MS
TFC6 YDR362C Subunit of RNA polymerase III transcription initiation factor complex physical 1 1 1 Affinity Capture-MS
SIS1 YNL007C Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p physical 1 1 1 Affinity Capture-MS
RPS0A YGR214W Ribosomal 40S subunit protein S0A physical 1 1 1 Affinity Capture-MS
RPS1A YLR441C Ribosomal protein of the small (40S) subunit physical 1 1 1 Affinity Capture-MS
RPS2 YGL123W Protein component of the small (40S) subunit physical 1 1 1 Affinity Capture-MS
RPS7A YOR096W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS8A YBL072C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS10A YOR293W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS12 YOR369C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS15 YOL040C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS19A YOL121C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS20 YHL015W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS22A YJL190C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS27A YKL156W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS3 YNL178W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS14A YCR031C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
TAF1 YGR274C TFIID subunit, involved in RNA pol II transcription initiation genetic 1 1 1 Synthetic Lethality
TTI1 YKL033W Subunit of the ASTRA complex, involved in chromatin remodeling genetic 1 1 1 Negative Genetic
UBX2 YML013W Bridging factor involved in ER-associated protein degradation (ERAD) genetic 1 1 1 Negative Genetic
RVS161 YCR009C Amphiphysin-like lipid raft protein genetic 1 1 1 Negative Genetic
SEC66 YBR171W Non-essential subunit of Sec63 complex genetic 1 1 1 Negative Genetic
SEC61 YLR378C Conserved ER protein translocation channel genetic 1 1 1 Negative Genetic
SMI1 YGR229C Protein involved in the regulation of cell wall synthesis genetic 1 1 1 Negative Genetic
UME6 YDR207C Rpd3L histone deacetylase complex subunit genetic 1 1 1 Negative Genetic
SHP1 YBL058W UBX domain-containing substrate adaptor for Cdc48p genetic 1 1 1 Negative Genetic
RPN4 YDL020C Transcription factor that stimulates expression of proteasome genes genetic 1 1 1 Dosage Rescue
PRE10 YOR362C Alpha 7 subunit of the 20S proteasome physical 1 1 1 Affinity Capture-MS
PRE6 YOL038W Alpha 4 subunit of the 20S proteasome physical 1 1 1 Affinity Capture-MS
PRE3 YJL001W Beta 1 subunit of the 20S proteasome physical 1 1 1 Affinity Capture-MS
ECM16 YMR128W Essential DEAH-box ATP-dependent RNA helicase specific to U3 snoRNP physical 1 1 1 Affinity Capture-MS
NAB2 YGL122C Nuclear polyadenylated RNA-binding protein physical 1 1 1 Affinity Capture-RNA
CDC28 YBR160W Cyclin-dependent kinase (CDK) catalytic subunit genetic 1 1 1 Synthetic Growth Defect
CKS1 YBR135W Cyclin-dependent protein kinase regulatory subunit and adaptor physical 1 1 1 Reconstituted Complex
CKS1 YBR135W Cyclin-dependent protein kinase regulatory subunit and adaptor genetic 1 1 1 Synthetic Growth Defect
FTH1 YBR207W Putative high affinity iron transporter physical 1 1 1 Affinity Capture-MS
DHH1 YDL160C Cytoplasmic DEAD-box helicase and mRNA decapping activator physical 1 1 1 Affinity Capture-RNA
ISW1 YBR245C ATPase subunit of imitation-switch (ISWI) class chromatin remodelers physical 1 1 1 Affinity Capture-RNA
KRR1 YCL059C Nucleolar protein required for rRNA synthesis and ribosomal assembly physical 1 1 1 Affinity Capture-MS
FAB1 YFR019W 1-phosphatidylinositol-3-phosphate 5-kinase physical 1 1 1 Affinity Capture-MS
HSC82 YMR186W Cytoplasmic chaperone of the Hsp90 family physical 1 1 1 Affinity Capture-MS
GIM3 YNL153C Subunit of the heterohexameric cochaperone prefoldin complex genetic 1 1 1 Positive Genetic
PAC10 YGR078C Part of the heteromeric co-chaperone GimC/prefoldin complex genetic 1 1 1 Positive Genetic
HSP82 YPL240C Hsp90 chaperone physical 1 1 1 Affinity Capture-MS
HSP82 YPL240C Hsp90 chaperone genetic 1 1 1 Synthetic Growth Defect
IMP4 YNL075W Component of the SSU processome physical 1 1 1 Affinity Capture-MS
RPN13 YLR421C Subunit of the 19S regulatory particle of the 26S proteasome lid physical 1 1 1 Affinity Capture-MS
NAT1 YDL040C Subunit of protein N-terminal acetyltransferase NatA physical 1 1 1 Affinity Capture-MS
CAF20 YOR276W Phosphoprotein of the mRNA cap-binding complex physical 1 1 1 Affinity Capture-MS
ARF1 YDL192W ADP-ribosylation factor genetic 1 1 1 Negative Genetic
PMA1 YGL008C Plasma membrane P2-type H+-ATPase genetic 1 1 1 Negative Genetic
ATG12 YBR217W Ubiquitin-like modifier involved in autophagy and the Cvt pathway physical 1 1 1 Affinity Capture-MS
HSP104 YLL026W Disaggregase physical 1 1 1 Affinity Capture-MS
DSS1 YMR287C 3'-5' exoribonuclease physical 1 1 1 Affinity Capture-Western
EST1 YLR233C TLC1 RNA-associated factor involved in telomere length regulation physical 1 1 1 Affinity Capture-MS
PGM1 YKL127W Phosphoglucomutase, minor isoform genetic 1 1 1 Negative Genetic
HAL5 YJL165C Snf1p-related nutrient-responsive protein kinase physical 1 1 1 Affinity Capture-MS
CLB2 YPR119W B-type cyclin involved in cell cycle progression physical 1 1 1 Affinity Capture-Western
LAA1 YJL207C AP-1 accessory protein physical 1 1 1 Affinity Capture-MS
DEF1 YKL054C RNAPII degradation factor physical 1 1 1 Affinity Capture-MS
MMS22 YLR320W Subunit of E3 ubiquitin ligase complex involved in replication repair physical 1 1 1 Affinity Capture-MS
MLP2 YIL149C Myosin-like protein associated with the nuclear envelope physical 1 1 1 Affinity Capture-MS
NAS2 YIL007C Evolutionarily conserved 19S regulatory particle assembly-chaperone genetic 1 1 1 Phenotypic Enhancement
ADE2 YOR128C Phosphoribosylaminoimidazole carboxylase physical 1 1 1 Affinity Capture-MS
FUI1 YBL042C High affinity uridine permease, localizes to the plasma membrane physical 1 1 1 Two-hybrid
ALY2 YJL084C Alpha arrestin, Ub-ligase adaptor for Rsp5p physical 1 1 1 Affinity Capture-MS
PRP5 YBR237W RNA helicase in the DEAD-box family physical 1 1 1 Affinity Capture-MS
MND2 YIR025W Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) physical 1 1 1 PCA
RGA1 YOR127W GTPase-activating protein for polarity-regulator Cdc42p (RhoGAP) physical 1 1 1 PCA
CSE4 YKL049C Centromeric histone H3-like protein physical 1 1 1 Affinity Capture-MS
DBF4 YDR052C Regulatory subunit of Cdc7p-Dbf4p kinase complex physical 1 1 1 Affinity Capture-MS
MAD1 YGL086W Coiled-coil protein involved in spindle-assembly checkpoint genetic 1 1 1 Synthetic Lethality
HRB1 YNL004W Poly(A+) RNA-binding protein physical 1 1 1 Affinity Capture-MS
MPT5 YGL178W mRNA-binding protein of the PUF family physical 1 1 1 PCA
CSG2 YBR036C Endoplasmic reticulum membrane protein genetic 1 1 1 Positive Genetic
ESC1 YMR219W Protein involved in telomeric silencing physical 1 1 1 Affinity Capture-MS
RGA2 YDR379W GTPase-activating protein for polarity-establishment protein Cdc42p physical 1 1 1 Affinity Capture-MS
CLA4 YNL298W Cdc42p-activated signal transducing kinase genetic 1 1 1 Negative Genetic
RPL25 YOL127W Ribosomal 60S subunit protein L25 physical 1 1 1 Affinity Capture-MS
RPL31A YDL075W Ribosomal 60S subunit protein L31A physical 1 1 1 Affinity Capture-MS
MRC1 YCL061C S-phase checkpoint protein required for DNA replication physical 1 1 1 Affinity Capture-MS
BUL2 YML111W Alpha-arrestin, component of the Rsp5p E3-ubiquitin ligase complex physical 1 1 1 Affinity Capture-MS
CUZ1 YNL155W Protein with a role in the ubiquitin-proteasome pathway physical 1 1 1 Affinity Capture-MS
PTH2 YBL057C One of two mitochondrially-localized peptidyl-tRNA hydrolases physical 1 1 1 Affinity Capture-MS
PEX32 YBR168W Peroxisomal integral membrane protein physical 1 1 1 Affinity Capture-MS
BSC2 YDR275W Protein of unknown function involved in multidrug resistance physical 1 1 1 Affinity Capture-MS
CWH41 YGL027C Processing alpha glucosidase I physical 1 1 1 Affinity Capture-MS
RMA1 YKL132C Putative dihydrofolate synthetase physical 1 1 1 Affinity Capture-MS
RPL26A YLR344W Ribosomal 60S subunit protein L26A physical 1 1 1 Affinity Capture-MS
APJ1 YNL077W Hsp40 chaperone with a role in SUMO-mediated protein degradation physical 1 1 1 Affinity Capture-MS
POX1 YGL205W Fatty-acyl coenzyme A oxidase physical 1 1 1 PCA
HER2 YMR293C Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex physical 1 1 1 PCA
CCW12 YLR110C Cell wall mannoprotein genetic 1 1 1 Negative Genetic