Standard name Systematic name Name description Description
RFA3 YJL173C Subunit of heterotrimeric Replication Protein A (RPA) Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress
Visualize the interactome of RFA3
Links to external databases:
SGD   |   KEGG   |  

Cell cycle phase and timing of transcription peak

Cell cycle phase Time (min)
G1/S 54

CYCLoPs localization & abundance

WT1 WT2 WT3
Mito 0.061 NaN 0.106
Nucleolus 0.351 0.347 0.300
Nucleus 0.611 0.604 0.612
Nuc Periphery NaN 0.059 NaN

GFP localization & abundance

GFP localization GFP abundance
nucleus 4280

GO term count

Metabolism Cell cycle Cell division Signal transduction DNA replication
RFA3 10 1 0 0 2

List of interactors

Use the search utility to find the interactor you are looking for. By clicking the column headers the table will be sorted on that column. Use shift+click to sort on multiple columns. Default sorting is on number of experiments, number of unique methods and the number of publications, and alphabetical on standard name, in that order.
Standard name Systematic name Name description Type #Experiments #Methods #Publications Evidence
RFA1 YAR007C Subunit of heterotrimeric Replication Protein A (RPA) physical 16 5 13 Co-purification, Affinity Capture-MS, Affinity Capture-MS, Two-hybrid, Two-hybrid, Two-hybrid, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Two-hybrid, Affinity Capture-Western, Affinity Capture-Western, Co-purification, Affinity Capture-MS
RFA2 YNL312W Subunit of heterotrimeric Replication Protein A (RPA) physical 10 4 8 Affinity Capture-MS, Affinity Capture-MS, Two-hybrid, Two-hybrid, Two-hybrid, Reconstituted Complex, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-Western
RAD52 YML032C Protein involved in homologous recombination physical 4 3 3 Two-hybrid, Affinity Capture-MS, Two-hybrid, Reconstituted Complex
FKH1 YIL131C Forkhead family transcription factor regulation 2 2 2 chromatin immunoprecipitation-chip evidence, ChEC-Seq experiments
RAD52 YML032C Protein involved in homologous recombination genetic 2 2 2 Negative Genetic, Phenotypic Enhancement
RFA2 YNL312W Subunit of heterotrimeric Replication Protein A (RPA) genetic 2 2 2 Synthetic Lethality, Dosage Rescue
MEC1 YBR136W Genome integrity checkpoint protein and PI kinase superfamily member physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
MPH1 YIR002C 3'-5' DNA helicase involved in error-free bypass of DNA lesions physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
MSH6 YDR097C Protein required for mismatch repair in mitosis and meiosis physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
CMR1 YDL156W Nuclear protein with role in transcription, protein quality control physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
RTT105 YER104W Chaperone for Replication Protein A complex (RPA) physical 2 2 1 Affinity Capture-MS, Reconstituted Complex
KAP95 YLR347C Karyopherin beta physical 2 1 1 Affinity Capture-MS, Affinity Capture-MS
GCN4 YEL009C bZIP transcriptional activator of amino acid biosynthetic genes regulation 1 1 1 DNA to cDNA expression microarray evidence
GLN3 YER040W Transcriptional activator in nitrogen catabolite repression system regulation 1 1 1 chromatin immunoprecipitation-chip evidence
PUT3 YKL015W Transcriptional activator regulation 1 1 1 chromatin immunoprecipitation-chip evidence
DSN1 YIR010W Essential component of the outer kinetochore MIND complex physical 1 1 1 Affinity Capture-MS
POB3 YML069W Subunit of the heterodimeric FACT complex (Spt16p-Pob3p) genetic 1 1 1 Negative Genetic
NIP7 YPL211W Nucleolar protein required for 60S ribosome subunit biogenesis genetic 1 1 1 Negative Genetic
BRE1 YDL074C E3 ubiquitin ligase genetic 1 1 1 Negative Genetic
MES1 YGR264C Methionyl-tRNA synthetase genetic 1 1 1 Negative Genetic
PAT1 YCR077C Deadenylation-dependent mRNA-decapping factor genetic 1 1 1 Positive Genetic
LSM6 YDR378C Lsm (Like Sm) protein genetic 1 1 1 Positive Genetic
NOP12 YOL041C Nucleolar protein involved in pre-25S rRNA processing genetic 1 1 1 Negative Genetic
REI1 YBR267W Cytoplasmic pre-60S factor genetic 1 1 1 Negative Genetic
HCR1 YLR192C eIF3j component of translation initiation factor 3 (eIF3) genetic 1 1 1 Positive Genetic
ACT1 YFL039C Actin, structural protein in cytoskeleton genetic 1 1 1 Negative Genetic
AAH1 YNL141W Adenine deaminase (adenine aminohydrolase) genetic 1 1 1 Negative Genetic
ALA1 YOR335C Cytoplasmic and mitochondrial alanyl-tRNA synthetase genetic 1 1 1 Negative Genetic
NAB2 YGL122C Nuclear polyadenylated RNA-binding protein physical 1 1 1 Affinity Capture-RNA
ARC15 YIL062C Subunit of the ARP2/3 complex genetic 1 1 1 Negative Genetic
ARC35 YNR035C Subunit of the ARP2/3 complex genetic 1 1 1 Negative Genetic
ARO1 YDR127W Pentafunctional arom protein physical 1 1 1 Affinity Capture-MS
CDC24 YAL041W Guanine nucleotide exchange factor (GEF) for Cdc42p genetic 1 1 1 Negative Genetic
CTF18 YMR078C Subunit of a complex with Ctf8p genetic 1 1 1 Negative Genetic
CYS4 YGR155W Cystathionine beta-synthase physical 1 1 1 Affinity Capture-MS
DNA2 YHR164C ATP-dependent 5'-flap endonuclease and DNA helicase physical 1 1 1 Affinity Capture-MS
DOA1 YKL213C WD-repeat protein involved in ubiquitin-mediated protein degradation genetic 1 1 1 Negative Genetic
EMG1 YLR186W Methyltransferase for rRNA genetic 1 1 1 Negative Genetic
HHF1 YBR009C Histone H4 physical 1 1 1 Affinity Capture-MS
HHF2 YNL030W Histone H4 physical 1 1 1 Affinity Capture-MS
HTB2 YBL002W Histone H2B physical 1 1 1 Affinity Capture-MS
HSL1 YKL101W Nim1p-related protein kinase genetic 1 1 1 Negative Genetic
MOT1 YPL082C Essential protein involved in regulation of transcription physical 1 1 1 Affinity Capture-Western
HDA3 YPR179C Subunit of HDA1 histone deacetylase complex genetic 1 1 1 Negative Genetic
MSH3 YCR092C Mismatch repair protein physical 1 1 1 Affinity Capture-MS
MUM2 YBR057C Protein essential for meiotic DNA replication and sporulation genetic 1 1 1 Negative Genetic
MYO2 YOR326W Type V myosin motor involved in actin-based transport of cargos genetic 1 1 1 Negative Genetic
HTA1 YDR225W Histone H2A physical 1 1 1 Affinity Capture-MS
NAB3 YPL190C RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p) genetic 1 1 1 Negative Genetic
HTA2 YBL003C Histone H2A physical 1 1 1 Affinity Capture-MS
MVD1 YNR043W Mevalonate pyrophosphate decarboxylase genetic 1 1 1 Negative Genetic
NOP14 YDL148C Nucleolar protein genetic 1 1 1 Negative Genetic
PRE9 YGR135W Alpha 3 subunit of the 20S proteasome genetic 1 1 1 Positive Genetic
RAD51 YER095W Strand exchange protein physical 1 1 1 Reconstituted Complex
RAD51 YER095W Strand exchange protein genetic 1 1 1 Negative Genetic
RNH1 YMR234W Ribonuclease H1 physical 1 1 1 Affinity Capture-MS
RFA3 YJL173C Subunit of heterotrimeric Replication Protein A (RPA) physical 1 1 1 Two-hybrid
SLI15 YBR156C Subunit of the conserved chromosomal passenger complex (CPC) physical 1 1 1 Affinity Capture-MS
SMC4 YLR086W Subunit of the condensin complex physical 1 1 1 Affinity Capture-MS
SPB4 YFL002C Putative ATP-dependent RNA helicase physical 1 1 1 Affinity Capture-MS
SRO9 YCL037C Cytoplasmic RNA-binding protein physical 1 1 1 Affinity Capture-RNA
SSB2 YNL209W Cytoplasmic ATPase that is a ribosome-associated molecular chaperone physical 1 1 1 Co-purification
VAM6 YDL077C Guanine nucleotide exchange factor for the GTPase Gtr1p physical 1 1 1 Affinity Capture-MS
TOM1 YDR457W E3 ubiquitin ligase of the hect-domain class physical 1 1 1 Affinity Capture-MS
RVB1 YDR190C ATP-dependent DNA helicase, also known as pontin physical 1 1 1 Affinity Capture-MS
RPC82 YPR190C RNA polymerase III subunit C82 physical 1 1 1 Affinity Capture-MS
STT3 YGL022W Catalytic subunit of the oligosaccharyltransferase complex of the ER l genetic 1 1 1 Positive Genetic
RFC2 YJR068W Subunit of heteropentameric Replication factor C (RF-C) genetic 1 1 1 Positive Genetic
VTI1 YMR197C Protein involved in cis-Golgi membrane traffic genetic 1 1 1 Positive Genetic
SAC7 YDR389W GTPase activating protein (GAP) for Rho1p genetic 1 1 1 Positive Genetic
RPL43A YPR043W Ribosomal 60S subunit protein L43A genetic 1 1 1 Positive Genetic
SEC18 YBR080C AAA ATPase and SNARE disassembly chaperone genetic 1 1 1 Negative Genetic
RSC6 YCR052W Component of the RSC chromatin remodeling complex genetic 1 1 1 Negative Genetic
RRP1 YDR087C Essential evolutionarily conserved nucleolar protein genetic 1 1 1 Negative Genetic
RLI1 YDR091C Essential Fe-S protein genetic 1 1 1 Negative Genetic
SUP35 YDR172W Translation termination factor eRF3 genetic 1 1 1 Negative Genetic
RSP5 YER125W NEDD4 family E3 ubiquitin ligase genetic 1 1 1 Negative Genetic
SEC62 YPL094C Essential subunit of Sec63 complex genetic 1 1 1 Negative Genetic
SCL1 YGL011C Alpha 1 subunit of the 20S proteasome genetic 1 1 1 Negative Genetic
RPT6 YGL048C ATPase of the 19S regulatory particle of the 26S proteasome genetic 1 1 1 Negative Genetic
URA6 YKL024C Uridylate kinase genetic 1 1 1 Negative Genetic
SEC65 YML105C Subunit of the signal recognition particle (SRP) genetic 1 1 1 Negative Genetic
SUA7 YPR086W Transcription factor TFIIB genetic 1 1 1 Negative Genetic
UBC4 YBR082C Ubiquitin-conjugating enzyme (E2) genetic 1 1 1 Negative Genetic
SGF29 YCL010C Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes genetic 1 1 1 Negative Genetic
RPP1A YDL081C Ribosomal stalk protein P1 alpha genetic 1 1 1 Negative Genetic
SWI5 YDR146C Transcription factor that recruits Mediator and Swi/Snf complexes genetic 1 1 1 Negative Genetic
TRM82 YDR165W Noncatalytic subunit of a tRNA methyltransferase complex genetic 1 1 1 Negative Genetic
SDH4 YDR178W Membrane anchor subunit of succinate dehydrogenase (SDH) genetic 1 1 1 Negative Genetic
VPS72 YDR485C Htz1p-binding component of the SWR1 complex genetic 1 1 1 Negative Genetic
ZNF1 YFL052W Zinc cluster transcription factor that regulates respiratory growth genetic 1 1 1 Negative Genetic
UBP6 YFR010W Ubiquitin-specific protease genetic 1 1 1 Negative Genetic
SAP155 YFR040W Protein required for function of the Sit4p protein phosphatase genetic 1 1 1 Negative Genetic
YGL036W YGL036W Putative protein of unknown function genetic 1 1 1 Negative Genetic
SAE2 YGL175C Endonuclease required for telomere elongation genetic 1 1 1 Negative Genetic
RTF1 YGL244W Subunit of RNAPII-associated chromatin remodeling Paf1 complex genetic 1 1 1 Negative Genetic
YTA7 YGR270W Chromatin-binding ATPase that regulates histone gene expression genetic 1 1 1 Negative Genetic
RTT107 YHR154W BRCA1 C-terminal domain protein needed for recovery from DNA damage genetic 1 1 1 Negative Genetic
RPN10 YHR200W Proteasome polyubiquitin receptor genetic 1 1 1 Negative Genetic
RPL14A YKL006W Ribosomal 60S subunit protein L14A genetic 1 1 1 Negative Genetic
RTT109 YLL002W Histone acetyltransferase genetic 1 1 1 Negative Genetic
RPL22A YLR061W Ribosomal 60S subunit protein L22A genetic 1 1 1 Negative Genetic
SLX4 YLR135W Endonuclease involved in processing DNA genetic 1 1 1 Negative Genetic
VIP1 YLR410W Bifunctional inositol pyrophosphate kinase and phosphatase genetic 1 1 1 Negative Genetic
SST2 YLR452C GTPase-activating protein for Gpa1p genetic 1 1 1 Negative Genetic
TSA1 YML028W Thioredoxin peroxidase genetic 1 1 1 Negative Genetic
SUB1 YMR039C Transcriptional regulator genetic 1 1 1 Negative Genetic
RTG1 YOL067C Transcription factor (bHLH) involved in interorganelle communication genetic 1 1 1 Negative Genetic
RTS1 YOR014W B-type regulatory subunit of protein phosphatase 2A (PP2A) genetic 1 1 1 Negative Genetic
VPS4 YPR173C AAA-ATPase involved in multivesicular body (MVB) protein sorting genetic 1 1 1 Negative Genetic
NUT2 YPR168W Subunit of the RNA polymerase II mediator complex genetic 1 1 1 Negative Genetic
MNN10 YDR245W Subunit of a Golgi mannosyltransferase complex genetic 1 1 1 Negative Genetic
PRP9 YDL030W Subunit of the SF3a splicing factor complex genetic 1 1 1 Negative Genetic
MKS1 YNL076W Pleiotropic negative transcriptional regulator genetic 1 1 1 Negative Genetic
ABP1 YCR088W Actin-binding protein of the cortical actin cytoskeleton genetic 1 1 1 Negative Genetic
AAC3 YBR085W Mitochondrial inner membrane ADP/ATP translocator physical 1 1 1 Affinity Capture-MS
GPP1 YIL053W Constitutively expressed DL-glycerol-3-phosphate phosphatase physical 1 1 1 Affinity Capture-MS
RAD54 YGL163C DNA-dependent ATPase that stimulates strand exchange genetic 1 1 1 Negative Genetic
HTB1 YDR224C Histone H2B physical 1 1 1 Affinity Capture-MS
GSP1 YLR293C Ran GTPase genetic 1 1 1 Negative Genetic
PGM2 YMR105C Phosphoglucomutase physical 1 1 1 Affinity Capture-MS
COF1 YLL050C Cofilin, involved in pH-dependent actin filament depolarization genetic 1 1 1 Negative Genetic
HXT7 YDR342C High-affinity glucose transporter physical 1 1 1 Affinity Capture-MS
RAD55 YDR076W Protein that stimulates strand exchange genetic 1 1 1 Negative Genetic
HSP104 YLL026W Disaggregase physical 1 1 1 Affinity Capture-MS
HEF3 YNL014W Translational elongation factor EF-3 physical 1 1 1 Affinity Capture-MS
MTC5 YDR128W Subunit of SEACAT, a subcomplex of the SEA complex physical 1 1 1 Affinity Capture-MS
MET30 YIL046W F-box protein containing five copies of the WD40 motif genetic 1 1 1 Negative Genetic
GPP2 YER062C DL-glycerol-3-phosphate phosphatase involved in glycerol biosynthesis physical 1 1 1 Affinity Capture-MS
IPA1 YJR141W Protein implicated in pre-mRNA processing and proteasomal degradation physical 1 1 1 Affinity Capture-MS
CDC37 YDR168W Essential Hsp90p co-chaperone genetic 1 1 1 Negative Genetic
HHT1 YBR010W Histone H3 physical 1 1 1 Affinity Capture-MS
CTF4 YPR135W Chromatin-associated protein genetic 1 1 1 Negative Genetic
MRE11 YMR224C Nuclease subunit of the MRX complex with Rad50p and Xrs2p genetic 1 1 1 Negative Genetic
RAD59 YDL059C Protein involved DNA double-strand break repair genetic 1 1 1 Phenotypic Enhancement
CLB2 YPR119W B-type cyclin involved in cell cycle progression genetic 1 1 1 Negative Genetic
ELG1 YOR144C Subunit of an alternative replication factor C complex genetic 1 1 1 Negative Genetic
FKH2 YNL068C Forkhead family transcription factor genetic 1 1 1 Negative Genetic
HOM2 YDR158W Aspartic beta semi-aldehyde dehydrogenase genetic 1 1 1 Negative Genetic
BIR1 YJR089W Subunit of chromosomal passenger complex (CPC) physical 1 1 1 Affinity Capture-MS
MMS22 YLR320W Subunit of E3 ubiquitin ligase complex involved in replication repair genetic 1 1 1 Negative Genetic
NBP2 YDR162C Protein involved in the HOG (high osmolarity glycerol) pathway genetic 1 1 1 Negative Genetic
DAD1 YDR016C Essential subunit of the Dam1 complex (aka DASH complex) genetic 1 1 1 Negative Genetic
BUD27 YFL023W Unconventional prefoldin protein involved in translation initiation genetic 1 1 1 Negative Genetic
CLN3 YAL040C G1 cyclin involved in cell cycle progression genetic 1 1 1 Negative Genetic
HST3 YOR025W Sirtuin, histone H3 deacetylase genetic 1 1 1 Negative Genetic
IPK1 YDR315C Inositol 1,3,4,5,6-pentakisphosphate 2-kinase genetic 1 1 1 Negative Genetic
MOB2 YFL034C-B Activator of Cbk1p kinase genetic 1 1 1 Positive Genetic
CSM3 YMR048W Replication fork associated factor genetic 1 1 1 Negative Genetic
BAS1 YKR099W Myb-related transcription factor genetic 1 1 1 Negative Genetic
ARP6 YLR085C Actin-related protein that binds nucleosomes genetic 1 1 1 Negative Genetic
RAD9 YDR217C DNA damage-dependent checkpoint protein genetic 1 1 1 Negative Genetic
CTF8 YHR191C Subunit of a complex with Ctf18p genetic 1 1 1 Negative Genetic
PHO80 YOL001W Cyclin genetic 1 1 1 Positive Genetic
RAD17 YOR368W Checkpoint protein genetic 1 1 1 Negative Genetic
NMD2 YHR077C Protein involved in the nonsense-mediated mRNA decay (NMD) pathway genetic 1 1 1 Synthetic Growth Defect
HSF1 YGL073W Trimeric heat shock transcription factor genetic 1 1 1 Negative Genetic
POL32 YJR043C Third subunit of DNA polymerase delta genetic 1 1 1 Negative Genetic
POL3 YDL102W Catalytic subunit of DNA polymerase delta genetic 1 1 1 Negative Genetic
PFY1 YOR122C Profilin genetic 1 1 1 Negative Genetic
HHT2 YNL031C Histone H3 physical 1 1 1 Affinity Capture-MS
IXR1 YKL032C Transcriptional repressor that regulates hypoxic genes during normoxia genetic 1 1 1 Positive Genetic
MRC1 YCL061C S-phase checkpoint protein required for DNA replication genetic 1 1 1 Negative Genetic
MMS1 YPR164W Subunit of E3 ubiquitin ligase complex involved in replication repair genetic 1 1 1 Negative Genetic
DCC1 YCL016C Subunit of a complex with Ctf8p and Ctf18p genetic 1 1 1 Negative Genetic
PDC2 YDR081C Transcription factor for thiamine-regulated genes genetic 1 1 1 Negative Genetic
RAD27 YKL113C 5' to 3' exonuclease, 5' flap endonuclease genetic 1 1 1 Negative Genetic
POC4 YPL144W Component of a heterodimeric Poc4p-Irc25p chaperone genetic 1 1 1 Positive Genetic
GIS2 YNL255C Translational activator for mRNAs with internal ribosome entry sites physical 1 1 1 Affinity Capture-MS
APJ1 YNL077W Hsp40 chaperone with a role in SUMO-mediated protein degradation physical 1 1 1 Affinity Capture-MS
RAD57 YDR004W Protein that stimulates strand exchange genetic 1 1 1 Negative Genetic
MSA1 YOR066W Activator of G1-specific transcription factors MBF and SBF genetic 1 1 1 Negative Genetic
NEO1 YIL048W Phospholipid translocase (flippase) genetic 1 1 1 Negative Genetic
LGE1 YPL055C Protein involved in histone H2B ubiquitination physical 1 1 1 Affinity Capture-MS
LGE1 YPL055C Protein involved in histone H2B ubiquitination genetic 1 1 1 Negative Genetic
CDC1 YDR182W Putative mannose-ethanolamine phosphate phosphodiesterase genetic 1 1 1 Negative Genetic
MMM1 YLL006W ER integral membrane protein, ERMES complex subunit physical 1 1 1 Affinity Capture-MS
GPI11 YDR302W ER membrane protein involved in a late step of GPI anchor assembly genetic 1 1 1 Negative Genetic