Standard name Systematic name Name description Description
SPC25 YER018C Component of the kinetochore-associated Ndc80 complex Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p
Visualize the interactome of SPC25
Links to external databases:
SGD   |   KEGG   |  

Cell cycle phase and timing of transcription peak

Cell cycle phase Time (min)
G1/S 42

CYCLoPs localization & abundance

WT1 WT2 WT3
Endosome 0.097 NaN 0.085
Mito 0.059 NaN NaN
Nucleolus 0.504 0.441 0.281
Peroxisome 0.090 NaN NaN
SpindlePole 0.895 0.819 0.868

GFP localization & abundance

GFP localization GFP abundance
spindle pole 3280

GO term count

Metabolism Cell cycle Cell division Signal transduction DNA replication
SPC25 0 0 0 0 0

List of interactors

Use the search utility to find the interactor you are looking for. By clicking the column headers the table will be sorted on that column. Use shift+click to sort on multiple columns. Default sorting is on number of experiments, number of unique methods and the number of publications, and alphabetical on standard name, in that order.
Standard name Systematic name Name description Type #Experiments #Methods #Publications Evidence
SPC24 YMR117C Component of the kinetochore-associated Ndc80 complex physical 27 7 21 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Two-hybrid, Two-hybrid, Two-hybrid, Two-hybrid, Two-hybrid, Reconstituted Complex, Affinity Capture-MS, Affinity Capture-MS, Co-crystal Structure, Two-hybrid, Reconstituted Complex, Two-hybrid, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-Western, PCA, Two-hybrid, Reconstituted Complex, PCA, Reconstituted Complex, FRET
NDC80 YIL144W Component of the kinetochore-associated Ndc80 complex physical 16 6 13 Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Two-hybrid, Co-fractionation, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Two-hybrid, Co-purification, Reconstituted Complex, Reconstituted Complex, Reconstituted Complex, Reconstituted Complex
DSN1 YIR010W Essential component of the outer kinetochore MIND complex physical 10 4 9 Affinity Capture-MS, Two-hybrid, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, FRET, Affinity Capture-MS, Affinity Capture-MS
CNN1 YFR046C Kinetochore protein physical 10 4 6 Two-hybrid, Two-hybrid, Affinity Capture-MS, Co-purification, Affinity Capture-MS, Reconstituted Complex, Reconstituted Complex, Two-hybrid, Affinity Capture-MS, Co-purification
NUF2 YOL069W Component of the kinetochore-associated Ndc80 complex physical 9 4 6 Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Two-hybrid, Two-hybrid, Affinity Capture-MS, Two-hybrid, Affinity Capture-Western, Reconstituted Complex
SPC24 YMR117C Component of the kinetochore-associated Ndc80 complex genetic 4 3 3 Dosage Rescue, Dosage Rescue, Synthetic Lethality, Negative Genetic
NDC80 YIL144W Component of the kinetochore-associated Ndc80 complex genetic 4 3 3 Dosage Rescue, Dosage Rescue, Synthetic Lethality, Negative Genetic
NUF2 YOL069W Component of the kinetochore-associated Ndc80 complex genetic 3 3 3 Dosage Rescue, Synthetic Lethality, Negative Genetic
MTW1 YAL034W-A Essential component of the MIND kinetochore complex physical 3 2 3 Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western
NNF1 YJR112W Essential component of the outer kinetochore MIND complex physical 3 2 2 Two-hybrid, PCA, Two-hybrid
FKH1 YIL131C Forkhead family transcription factor regulation 2 2 2 chromatin immunoprecipitation-chip evidence, ChEC-Seq experiments
SPC105 YGL093W Subunit of a kinetochore-microtubule binding complex physical 2 2 2 Two-hybrid, FRET
DYN1 YKR054C Cytoplasmic heavy chain dynein physical 2 1 2 Two-hybrid, Two-hybrid
ADA2 YDR448W Transcription coactivator physical 2 1 2 Two-hybrid, Two-hybrid
MUM2 YBR057C Protein essential for meiotic DNA replication and sporulation physical 2 1 2 Two-hybrid, Two-hybrid
JNM1 YMR294W Component of the yeast dynactin complex physical 2 1 2 Two-hybrid, Two-hybrid
SPC25 YER018C Component of the kinetochore-associated Ndc80 complex physical 2 1 2 Two-hybrid, Two-hybrid
MAD1 YGL086W Coiled-coil protein involved in spindle-assembly checkpoint genetic 2 1 1 Synthetic Lethality, Synthetic Lethality
TYE7 YOR344C Serine-rich protein that contains a bHLH DNA binding motif physical 2 1 1 Two-hybrid, Two-hybrid
TED1 YIL039W GPI-glycan remodelase genetic 2 1 1 Phenotypic Enhancement, Phenotypic Enhancement
EMC2 YJR088C Member of conserved ER transmembrane complex genetic 2 1 1 Phenotypic Enhancement, Phenotypic Enhancement
SFP1 YLR403W Regulates transcription of ribosomal protein and biogenesis genes regulation 1 1 1 DNA to cDNA expression microarray evidence
FKH2 YNL068C Forkhead family transcription factor regulation 1 1 1 ChEC-Seq experiments
TFC7 YOR110W RNA pol III transcription initiation factor complex (TFIIIC) subunit regulation 1 1 1 chromatin immunoprecipitation-chip evidence
YAP5 YIR018W Basic leucine zipper (bZIP) iron-sensing transcription factor regulation 1 1 1 DNA to cDNA expression microarray evidence
LEU3 YLR451W Zinc-knuckle transcription factor, repressor and activator regulation 1 1 1 chromatin immunoprecipitation-chip evidence
CDC73 YLR418C Component of the Paf1p complex genetic 1 1 1 Synthetic Lethality
CEF1 YMR213W Essential splicing factor physical 1 1 1 Two-hybrid
AME1 YBR211C Essential kinetochore protein associated with microtubules and SPBs physical 1 1 1 Affinity Capture-MS
CTF19 YPL018W Outer kinetochore protein, needed for accurate chromosome segregation physical 1 1 1 Two-hybrid
DSN1 YIR010W Essential component of the outer kinetochore MIND complex genetic 1 1 1 Negative Genetic
NSL1 YPL233W Essential component of the MIND kinetochore complex physical 1 1 1 FRET
RTF1 YGL244W Subunit of RNAPII-associated chromatin remodeling Paf1 complex genetic 1 1 1 Negative Genetic
ARP4 YJL081C Nuclear actin-related protein involved in chromatin remodeling genetic 1 1 1 Negative Genetic
ESA1 YOR244W Catalytic subunit of the histone acetyltransferase complex (NuA4) genetic 1 1 1 Negative Genetic
NOP2 YNL061W rRNA m5C methyltransferase genetic 1 1 1 Negative Genetic
RRP1 YDR087C Essential evolutionarily conserved nucleolar protein genetic 1 1 1 Negative Genetic
IPI1 YHR085W Component of the Rix1 complex and possibly pre-replicative complexes genetic 1 1 1 Negative Genetic
PRP43 YGL120C RNA helicase in the DEAH-box family genetic 1 1 1 Negative Genetic
MCM21 YDR318W Component of the kinetochore sub-complex COMA physical 1 1 1 Two-hybrid
MCM21 YDR318W Component of the kinetochore sub-complex COMA genetic 1 1 1 Synthetic Growth Defect
PAT1 YCR077C Deadenylation-dependent mRNA-decapping factor physical 1 1 1 Two-hybrid
PRP40 YKL012W U1 snRNP protein involved in splicing physical 1 1 1 Two-hybrid
NAP1 YKR048C Histone chaperone and assembly chaperone for Rps6a/b physical 1 1 1 Two-hybrid
RPT6 YGL048C ATPase of the 19S regulatory particle of the 26S proteasome physical 1 1 1 Two-hybrid
CCR4 YAL021C Component of the CCR4-NOT transcriptional complex physical 1 1 1 Affinity Capture-RNA
NUP82 YJL061W Linker nucleoporin component of the nuclear pore complex (NPC) physical 1 1 1 Two-hybrid
ADE4 YMR300C Phosphoribosylpyrophosphate amidotransferase (PRPPAT) genetic 1 1 1 Negative Genetic
BDF1 YLR399C BET family transcriptional regulator physical 1 1 1 Two-hybrid
CDC11 YJR076C Component of the septin ring that is required for cytokinesis genetic 1 1 1 Positive Genetic
CDC36 YDL165W Component of the CCR4-NOT core complex, involved in mRNA decapping physical 1 1 1 Two-hybrid
CTK1 YKL139W Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I) genetic 1 1 1 Negative Genetic
EPL1 YFL024C Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex physical 1 1 1 Two-hybrid
DHH1 YDL160C Cytoplasmic DEAD-box helicase and mRNA decapping activator physical 1 1 1 Affinity Capture-RNA
KIN3 YAR018C Nonessential serine/threonine protein kinase physical 1 1 1 Two-hybrid
END3 YNL084C EH domain-containing protein involved in endocytosis physical 1 1 1 Two-hybrid
MBP1 YDL056W Transcription factor physical 1 1 1 Two-hybrid
MED6 YHR058C Subunit of the RNA polymerase II mediator complex physical 1 1 1 Two-hybrid
HDA2 YDR295C Subunit of HDA1 histone deacetylase complex physical 1 1 1 Two-hybrid
DAM1 YGR113W Essential subunit of the Dam1 complex (aka DASH complex) genetic 1 1 1 Negative Genetic
DEP1 YAL013W Component of the Rpd3L histone deacetylase complex physical 1 1 1 Two-hybrid
PHO23 YNL097C Component of the Rpd3L histone deacetylase complex physical 1 1 1 Two-hybrid
SDS3 YIL084C Component of the Rpd3L histone deacetylase complex physical 1 1 1 Two-hybrid
SIN3 YOL004W Component of both the Rpd3S and Rpd3L histone deacetylase complexes genetic 1 1 1 Positive Genetic
PRP9 YDL030W Subunit of the SF3a splicing factor complex physical 1 1 1 Two-hybrid
RPT5 YOR117W ATPase of the 19S regulatory particle of the 26S proteasome physical 1 1 1 Two-hybrid
SGF73 YGL066W Subunit of DUBm module of SAGA and SLIK physical 1 1 1 Two-hybrid
SGV1 YPR161C Cyclin (Bur2p)-dependent protein kinase genetic 1 1 1 Negative Genetic
HEK2 YBL032W RNA binding protein involved in asymmetric localization of ASH1 mRNA physical 1 1 1 Affinity Capture-RNA
CYM1 YDR430C Lysine-specific metalloprotease of the pitrilysin family genetic 1 1 1 Negative Genetic
DIA4 YHR011W Probable mitochondrial seryl-tRNA synthetase physical 1 1 1 Two-hybrid
PUF3 YLL013C Protein of the mitochondrial outer surface physical 1 1 1 Affinity Capture-RNA
FAR7 YFR008W Protein involved in recovery from pheromone-induced cell cycle arrest physical 1 1 1 Two-hybrid
GSP1 YLR293C Ran GTPase genetic 1 1 1 Negative Genetic
ARO4 YBR249C 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase physical 1 1 1 Two-hybrid
FKH1 YIL131C Forkhead family transcription factor physical 1 1 1 Two-hybrid
KOG1 YHR186C Subunit of TORC1 physical 1 1 1 Two-hybrid
CHK1 YBR274W Serine/threonine kinase and DNA damage checkpoint effector genetic 1 1 1 Negative Genetic
CMK1 YFR014C Calmodulin-dependent protein kinase genetic 1 1 1 Negative Genetic
PXR1 YGR280C Essential protein involved in rRNA and snoRNA maturation physical 1 1 1 Two-hybrid
SLX5 YDL013W Subunit of the Slx5-Slx8 SUMO-targeted Ub ligase (STUbL) complex physical 1 1 1 Two-hybrid
CYC8 YBR112C General transcriptional co-repressor physical 1 1 1 Two-hybrid
ETP1 YHL010C Protein of unknown function required for growth on ethanol physical 1 1 1 Two-hybrid
IPA1 YJR141W Protein implicated in pre-mRNA processing and proteasomal degradation genetic 1 1 1 Negative Genetic
GYP5 YPL249C GTPase-activating protein (GAP) for yeast Rab family members physical 1 1 1 Two-hybrid
ATG20 YDL113C Sorting nexin family member physical 1 1 1 Two-hybrid
CIK1 YMR198W Kinesin-associated protein physical 1 1 1 Two-hybrid
NOP53 YPL146C Nucleolar protein physical 1 1 1 Two-hybrid
RTT103 YDR289C Protein involved in transcription termination by RNA polymerase II genetic 1 1 1 Negative Genetic
MAS2 YHR024C Alpha subunit of the mitochondrial processing protease (MPP) genetic 1 1 1 Negative Genetic
RLF2 YPR018W Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1) physical 1 1 1 Two-hybrid
DAD1 YDR016C Essential subunit of the Dam1 complex (aka DASH complex) genetic 1 1 1 Negative Genetic
GZF3 YJL110C GATA zinc finger protein physical 1 1 1 Two-hybrid
BSC5 YNR069C Protein of unknown function physical 1 1 1 Two-hybrid
MCM16 YPR046W Component of the Ctf19 complex and the COMA subcomplex physical 1 1 1 Two-hybrid
MOB2 YFL034C-B Activator of Cbk1p kinase genetic 1 1 1 Negative Genetic
NDL1 YLR254C Homolog of nuclear distribution factor NudE physical 1 1 1 Two-hybrid
MND2 YIR025W Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) physical 1 1 1 Two-hybrid
ATG36 YJL185C Pex3p interacting protein, required for pexophagy physical 1 1 1 Two-hybrid
SLU7 YDR088C RNA splicing factor genetic 1 1 1 Negative Genetic
KEG1 YFR042W Integral membrane protein of the ER physical 1 1 1 Two-hybrid
ASK10 YGR097W Regulator of the Fps1p glycerol channel physical 1 1 1 Two-hybrid
SMC5 YOL034W Subunit of the SMC5-SMC6 complex genetic 1 1 1 Negative Genetic
APC9 YLR102C Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) genetic 1 1 1 Negative Genetic
CEP3 YMR168C Essential kinetochore protein genetic 1 1 1 Negative Genetic
CHL1 YPL008W Probable DNA helicase genetic 1 1 1 Synthetic Lethality
RSC30 YHR056C Component of the RSC chromatin remodeling complex physical 1 1 1 Two-hybrid
CDC21 YOR074C Thymidylate synthase genetic 1 1 1 Negative Genetic
SAC3 YDR159W mRNA export factor genetic 1 1 1 Negative Genetic
GCR1 YPL075W Transcriptional activator of genes involved in glycolysis physical 1 1 1 Two-hybrid
SNF6 YHL025W Subunit of the SWI/SNF chromatin remodeling complex physical 1 1 1 Two-hybrid
RPL22A YLR061W Ribosomal 60S subunit protein L22A genetic 1 1 1 Negative Genetic
BUB1 YGR188C Protein kinase involved in the cell cycle checkpoint into anaphase genetic 1 1 1 Synthetic Lethality
MAD1 YGL086W Coiled-coil protein involved in spindle-assembly checkpoint physical 1 1 1 Two-hybrid
MPS1 YDL028C Dual-specificity kinase physical 1 1 1 Affinity Capture-MS
RAD5 YLR032W DNA helicase/Ubiquitin ligase genetic 1 1 1 Dosage Lethality
SKN7 YHR206W Nuclear response regulator and transcription factor physical 1 1 1 Two-hybrid
CST9 YLR394W SUMO E3 ligase genetic 1 1 1 Negative Genetic
SPC110 YDR356W Inner plaque spindle pole body (SPB) component physical 1 1 1 Co-purification
DAL80 YKR034W Negative regulator of genes in multiple nitrogen degradation pathways physical 1 1 1 Two-hybrid
GPR1 YDL035C Plasma membrane G protein coupled receptor (GPCR) genetic 1 1 1 Negative Genetic
NNF2 YGR089W Protein that exhibits physical and genetic interactions with Rpb8p physical 1 1 1 Two-hybrid
UBC4 YBR082C Ubiquitin-conjugating enzyme (E2) physical 1 1 1 Two-hybrid
SWI5 YDR146C Transcription factor that recruits Mediator and Swi/Snf complexes physical 1 1 1 Two-hybrid
THP2 YHR167W Subunit of the THO and TREX complexes physical 1 1 1 Two-hybrid
SSP1 YHR184W Protein involved in the control of meiotic nuclear division physical 1 1 1 Two-hybrid
YLR031W YLR031W Putative protein of unknown function physical 1 1 1 Two-hybrid
SWI1 YPL016W Subunit of the SWI/SNF chromatin remodeling complex physical 1 1 1 Two-hybrid
YPL257W YPL257W Putative protein of unknown function physical 1 1 1 Two-hybrid
SPC34 YKR037C Essential subunit of the Dam1 complex (aka DASH complex) physical 1 1 1 Two-hybrid
YBP2 YGL060W Central kinetochore associated protein physical 1 1 1 Affinity Capture-Western
STU1 YBL034C Microtubule plus-end-tracking non-motor protein physical 1 1 1 Co-localization
SRO9 YCL037C Cytoplasmic RNA-binding protein physical 1 1 1 Affinity Capture-RNA
SPC72 YAL047C Gamma-tubulin small complex (gamma-TuSC) receptor physical 1 1 1 Two-hybrid
STP22 YCL008C Component of the ESCRT-I complex physical 1 1 1 Two-hybrid
STE50 YCL032W Adaptor protein for various signaling pathways physical 1 1 1 Two-hybrid
SSD1 YDR293C Translational repressor physical 1 1 1 Two-hybrid
VPS52 YDR484W Component of the GARP (Golgi-associated retrograde protein) complex physical 1 1 1 Two-hybrid
TSC11 YER093C Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p) physical 1 1 1 Two-hybrid
YKE2 YLR200W Subunit of the heterohexameric Gim/prefoldin protein complex physical 1 1 1 Two-hybrid
STB2 YMR053C Protein that interacts with Sin3p in a two-hybrid assay physical 1 1 1 Two-hybrid
UBR2 YLR024C Cytoplasmic ubiquitin-protein ligase (E3) physical 1 1 1 Affinity Capture-MS
SRP54 YPR088C Signal recognition particle (SRP) subunit (homolog of mammalian SRP54) physical 1 1 1 Co-purification
TAF1 YGR274C TFIID subunit, involved in RNA pol II transcription initiation genetic 1 1 1 Synthetic Lethality
TTI1 YKL033W Subunit of the ASTRA complex, involved in chromatin remodeling genetic 1 1 1 Negative Genetic
SRP101 YDR292C Signal recognition particle (SRP) receptor alpha subunit genetic 1 1 1 Negative Genetic
TAF6 YGL112C Subunit (60 kDa) of TFIID and SAGA complexes genetic 1 1 1 Negative Genetic
SUI2 YJR007W Alpha subunit of the translation initiation factor eIF2 genetic 1 1 1 Negative Genetic
SSC1 YJR045C Hsp70 family ATPase genetic 1 1 1 Negative Genetic
VAM6 YDL077C Guanine nucleotide exchange factor for the GTPase Gtr1p genetic 1 1 1 Negative Genetic
UME6 YDR207C Rpd3L histone deacetylase complex subunit genetic 1 1 1 Negative Genetic
SWE1 YJL187C Protein kinase that regulates the G2/M transition genetic 1 1 1 Negative Genetic
YME2 YMR302C Integral inner mitochondrial membrane protein genetic 1 1 1 Negative Genetic
VAM3 YOR106W Syntaxin-like vacuolar t-SNARE genetic 1 1 1 Negative Genetic
YTA7 YGR270W Chromatin-binding ATPase that regulates histone gene expression genetic 1 1 1 Synthetic Rescue
NIP100 YPL174C Large subunit of the dynactin complex physical 1 1 1 Two-hybrid
PAN3 YKL025C Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex physical 1 1 1 Two-hybrid
PAC1 YOR269W Regulator of dynein, involved in intracellular transport physical 1 1 1 Two-hybrid
OKP1 YGR179C Outer kinetochore protein required for accurate chromosome segregation genetic 1 1 1 Negative Genetic
ATP18 YML081C-A Subunit of the mitochondrial F1F0 ATP synthase genetic 1 1 1 Negative Genetic
ORC6 YHR118C Subunit of the origin recognition complex (ORC) genetic 1 1 1 Negative Genetic
SPC105 YGL093W Subunit of a kinetochore-microtubule binding complex genetic 1 1 1 Negative Genetic
DAD4 YDR320C-A Essential subunit of the Dam1 complex (aka DASH complex) physical 1 1 1 Two-hybrid
ASE1 YOR058C Mitotic spindle midzone-localized microtubule bundling protein physical 1 1 1 Two-hybrid
NSI1 YDR026C RNA polymerase I termination factor physical 1 1 1 Two-hybrid
SLF1 YDR515W RNA binding protein that associates with polysomes physical 1 1 1 Affinity Capture-RNA
AHK1 YDL073W Scaffold protein in the HKR1 sub-branch of the Hog1p-signaling pathway physical 1 1 1 Affinity Capture-MS
GEP7 YGL057C Protein of unknown function genetic 1 1 1 Negative Genetic
IAI11 YBL059W Putative mitochondrial protein of unknown function genetic 1 1 1 Negative Genetic
IRC3 YDR332W Double-stranded DNA-dependent helicase of the DExH/D-box family physical 1 1 1 Two-hybrid
SMT1 YJL147C Translational repressor of the mitochondrial ATP6/8 mRNA genetic 1 1 1 Negative Genetic
MDM36 YPR083W Mitochondrial protein physical 1 1 1 Two-hybrid
RDS2 YPL133C Transcription factor involved in regulating gluconeogenesis physical 1 1 1 Two-hybrid
PHB2 YGR231C Subunit of the prohibitin complex (Phb1p-Phb2p) genetic 1 1 1 Negative Genetic
RPS27B YHR021C Protein component of the small (40S) ribosomal subunit genetic 1 1 1 Negative Genetic
MDM34 YGL219C Mitochondrial component of the ERMES complex genetic 1 1 1 Negative Genetic
MFG1 YDL233W Regulator of filamentous growth physical 1 1 1 Two-hybrid
SOK2 YMR016C Nuclear protein that negatively regulates pseudohyphal differentiation physical 1 1 1 Two-hybrid
SDD4 YPR022C Putative transcription factor, as suggested by computational analysis physical 1 1 1 Two-hybrid
FRT1 YOR324C Tail-anchored ER membrane protein of unknown function physical 1 1 1 Two-hybrid
LAM4 YHR080C Sterol-binding protein that localizes to puncta in the cortical ER physical 1 1 1 Two-hybrid
MIC60 YKR016W Component of the MICOS complex genetic 1 1 1 Negative Genetic
MTC3 YGL226W Protein of unknown function genetic 1 1 1 Negative Genetic
RIB2 YOL066C Bifunctional DRAP deaminase tRNA:pseudouridine synthase genetic 1 1 1 Negative Genetic
HMS2 YJR147W Protein with similarity to heat shock transcription factors physical 1 1 1 Two-hybrid
PPM1 YDR435C Carboxyl methyltransferase genetic 1 1 1 Negative Genetic
IZH1 YDR492W Membrane protein involved in zinc ion homeostasis genetic 1 1 1 Negative Genetic