Standard name Systematic name Name description Description
TSR2 YLR435W Protein with a potential role in pre-rRNA processing Protein with a potential role in pre-rRNA processing
Visualize the interactome of TSR2
Links to external databases:
SGD   |   KEGG   |  

Cell cycle phase and timing of transcription peak

Cell cycle phase Time (min)

CYCLoPs localization & abundance

WT1 WT2 WT3
Nucleus 0.592 0.676 0.628
Vac/Vac Memb 0.472 0.364 0.372
Cytoplasm NaN 0.098 0.080

GFP localization & abundance

GFP localization GFP abundance
cytoplasm, nucleus 13400

GO term count

Metabolism Cell cycle Cell division Signal transduction DNA replication
TSR2 2 0 0 0 0

List of interactors

Use the search utility to find the interactor you are looking for. By clicking the column headers the table will be sorted on that column. Use shift+click to sort on multiple columns. Default sorting is on number of experiments, number of unique methods and the number of publications, and alphabetical on standard name, in that order.
Standard name Systematic name Name description Type #Experiments #Methods #Publications Evidence
RPS26B YER131W Protein component of the small (40S) ribosomal subunit physical 9 5 4 Two-hybrid, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-MS, Reconstituted Complex, Reconstituted Complex, Reconstituted Complex, Affinity Capture-Western, Co-crystal Structure
RPS26A YGL189C Protein component of the small (40S) ribosomal subunit physical 6 4 3 Two-hybrid, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-MS, Two-hybrid, Reconstituted Complex
SUP35 YDR172W Translation termination factor eRF3 genetic 2 2 2 Positive Genetic, Phenotypic Suppression
PUF4 YGL014W Member of the PUF protein family physical 2 2 2 Affinity Capture-MS, Affinity Capture-RNA
LSM1 YJL124C Lsm (Like Sm) protein genetic 2 1 2 Negative Genetic, Negative Genetic
PUF3 YLL013C Protein of the mitochondrial outer surface physical 2 1 2 Affinity Capture-RNA, Affinity Capture-RNA
SWC4 YGR002C Component of the Swr1p complex that incorporates Htz1p into chromatin genetic 2 1 1 Synthetic Lethality, Synthetic Lethality
KAP123 YER110C Karyopherin beta genetic 2 1 1 Synthetic Rescue, Synthetic Rescue
HDA1 YNL021W Catalytic subunit of class II HDA1 histone deacetylase complex genetic 2 1 1 Synthetic Growth Defect, Synthetic Growth Defect
RPD3 YNL330C Histone deacetylase, component of both the Rpd3S and Rpd3L complexes genetic 2 1 1 Synthetic Growth Defect, Synthetic Growth Defect
SFP1 YLR403W Regulates transcription of ribosomal protein and biogenesis genes regulation 1 1 1 DNA to cDNA expression microarray evidence
UGA3 YDL170W Transcriptional activator for GABA-dependent induction of GABA genes regulation 1 1 1 chromatin immunoprecipitation-chip evidence
CLF1 YLR117C Member of the NineTeen Complex (NTC) genetic 1 1 1 Negative Genetic
RPO21 YDL140C RNA polymerase II largest subunit B220 genetic 1 1 1 Positive Genetic
GLC7 YER133W Type 1 S/T protein phosphatase (PP1) catalytic subunit genetic 1 1 1 Negative Genetic
ESA1 YOR244W Catalytic subunit of the histone acetyltransferase complex (NuA4) genetic 1 1 1 Negative Genetic
BRX1 YOL077C Nucleolar protein genetic 1 1 1 Positive Genetic
RPL17A YKL180W Ribosomal 60S subunit protein L17A genetic 1 1 1 Positive Genetic
KAP123 YER110C Karyopherin beta physical 1 1 1 Reconstituted Complex
PRP45 YAL032C Protein required for pre-mRNA splicing genetic 1 1 1 Negative Genetic
NSE4 YDL105W Component of the SMC5-SMC6 complex genetic 1 1 1 Negative Genetic
NUG1 YER006W GTPase that associates with nuclear 60S pre-ribosomes genetic 1 1 1 Negative Genetic
RRP7 YCL031C Essential protein involved in rRNA processing and ribosome biogenesis genetic 1 1 1 Positive Genetic
NOP15 YNL110C Constituent of 66S pre-ribosomal particles genetic 1 1 1 Positive Genetic
ARC1 YGL105W Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases genetic 1 1 1 Positive Genetic
MES1 YGR264C Methionyl-tRNA synthetase genetic 1 1 1 Negative Genetic
MTR4 YJL050W RNA duplex-sensing translocase genetic 1 1 1 Negative Genetic
PAT1 YCR077C Deadenylation-dependent mRNA-decapping factor physical 1 1 1 Affinity Capture-RNA
PAT1 YCR077C Deadenylation-dependent mRNA-decapping factor genetic 1 1 1 Negative Genetic
TIF2 YJL138C Translation initiation factor eIF4A genetic 1 1 1 Negative Genetic
LRP1 YHR081W Nuclear exosome-associated nucleic acid binding protein genetic 1 1 1 Positive Genetic
DST1 YGL043W General transcription elongation factor TFIIS genetic 1 1 1 Negative Genetic
TIF34 YMR146C eIF3i subunit of the eukaryotic translation initiation factor 3 (eIF3) genetic 1 1 1 Negative Genetic
CCR4 YAL021C Component of the CCR4-NOT transcriptional complex physical 1 1 1 Affinity Capture-RNA
CDC39 YCR093W Subunit of the CCR4-NOT1 core complex genetic 1 1 1 Negative Genetic
KAP104 YBR017C Transportin or cytosolic karyopherin beta 2 physical 1 1 1 Reconstituted Complex
TSR2 YLR435W Protein with a potential role in pre-rRNA processing physical 1 1 1 Affinity Capture-MS
YRA1 YDR381W Nuclear polyadenylated RNA-binding protein physical 1 1 1 Affinity Capture-MS
ULP1 YPL020C Protease that specifically cleaves Smt3p protein conjugates physical 1 1 1 PCA
YKE2 YLR200W Subunit of the heterohexameric Gim/prefoldin protein complex genetic 1 1 1 Positive Genetic
VMA21 YGR105W Integral membrane protein required for V-ATPase function genetic 1 1 1 Synthetic Growth Defect
VOA1 YGR106C ER protein that functions in assembly of the V0 sector of V-ATPase genetic 1 1 1 Synthetic Growth Defect
UBC9 YDL064W SUMO-conjugating enzyme involved in the Smt3p conjugation pathway genetic 1 1 1 Positive Genetic
YPI1 YFR003C Regulatory subunit of the type I protein phosphatase (PP1) Glc7p genetic 1 1 1 Negative Genetic
YTH1 YPR107C Essential RNA-binding component of cleavage and polyadenylation factor genetic 1 1 1 Negative Genetic
VID24 YBR105C GID Complex regulatory subunit genetic 1 1 1 Negative Genetic
YOS9 YDR057W ER quality-control lectin genetic 1 1 1 Negative Genetic
VAC8 YEL013W Vacuole-specific Myo2p receptor genetic 1 1 1 Negative Genetic
YAT2 YER024W Carnitine acetyltransferase genetic 1 1 1 Negative Genetic
YGL188C-A YGL188C-A Putative protein of unknown function genetic 1 1 1 Negative Genetic
UBR1 YGR184C E3 ubiquitin ligase (N-recognin) genetic 1 1 1 Negative Genetic
UBC7 YMR022W Ubiquitin conjugating enzyme genetic 1 1 1 Negative Genetic
YMR090W YMR090W Putative protein of unknown function genetic 1 1 1 Negative Genetic
YPT11 YNL304W Rab GTPase genetic 1 1 1 Negative Genetic
YNR029C YNR029C Putative protein of unknown function genetic 1 1 1 Negative Genetic
YOR008C-A YOR008C-A Putative protein of unknown function genetic 1 1 1 Negative Genetic
YVC1 YOR087W Vacuolar cation channel genetic 1 1 1 Negative Genetic
YPL039W YPL039W Putative protein of unknown function genetic 1 1 1 Negative Genetic
YPL191C YPL191C K48-specific deubiquitinating (DUB) enzyme genetic 1 1 1 Negative Genetic
TUB4 YLR212C Gamma-tubulin genetic 1 1 1 Synthetic Rescue
CDC14 YFR028C Protein phosphatase required for mitotic exit genetic 1 1 1 Negative Genetic
CDC36 YDL165W Component of the CCR4-NOT core complex, involved in mRNA decapping genetic 1 1 1 Negative Genetic
CUS1 YMR240C Protein required for assembly of U2 snRNP into the spliceosome genetic 1 1 1 Negative Genetic
DCP2 YNL118C Catalytic subunit of Dcp1p-Dcp2p decapping enzyme complex genetic 1 1 1 Negative Genetic
CDC23 YHR166C Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) genetic 1 1 1 Negative Genetic
ERO1 YML130C Thiol oxidase required for oxidative protein folding in the ER genetic 1 1 1 Negative Genetic
GCN1 YGL195W Positive regulator of the Gcn2p kinase activity genetic 1 1 1 Negative Genetic
DHH1 YDL160C Cytoplasmic DEAD-box helicase and mRNA decapping activator physical 1 1 1 Affinity Capture-RNA
KRR1 YCL059C Nucleolar protein required for rRNA synthesis and ribosomal assembly genetic 1 1 1 Positive Genetic
FAP7 YDL166C Essential NTPase required for small ribosome subunit synthesis genetic 1 1 1 Dosage Rescue
MRPL35 YDR322W Mitochondrial ribosomal protein of the large subunit genetic 1 1 1 Negative Genetic
MRPL39 YML009C Mitochondrial ribosomal protein of the large subunit genetic 1 1 1 Negative Genetic
CMR1 YDL156W Nuclear protein with role in transcription, protein quality control genetic 1 1 1 Negative Genetic
MTR10 YOR160W Nuclear import receptor genetic 1 1 1 Positive Genetic
HTZ1 YOL012C Histone variant H2AZ genetic 1 1 1 Positive Genetic
LPD1 YFL018C Dihydrolipoamide dehydrogenase genetic 1 1 1 Negative Genetic
POP7 YBR167C Subunit of RNase MRP, nuclear RNase P and telomerase genetic 1 1 1 Positive Genetic
PSE1 YMR308C Karyopherin/importin that interacts with the nuclear pore complex physical 1 1 1 Reconstituted Complex
PHO23 YNL097C Component of the Rpd3L histone deacetylase complex genetic 1 1 1 Negative Genetic
NUP120 YKL057C Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC) genetic 1 1 1 Positive Genetic
SEC13 YLR208W Structural component of 3 complexes genetic 1 1 1 Synthetic Rescue
SAP30 YMR263W Component of Rpd3L histone deacetylase complex genetic 1 1 1 Negative Genetic
ECM25 YJL201W Endocytic site protein required for stress-induced cell elongation genetic 1 1 1 Negative Genetic
SLA1 YBL007C Cytoskeletal protein binding protein physical 1 1 1 Affinity Capture-MS
TFA1 YKL028W TFIIE large subunit genetic 1 1 1 Negative Genetic
TIF1 YKR059W Translation initiation factor eIF4A genetic 1 1 1 Negative Genetic
LTV1 YKL143W Subunit of the EGO/GSE complex genetic 1 1 1 Positive Genetic
RIO2 YNL207W Essential serine kinase involved in the processing of 20S pre-rRNA physical 1 1 1 Affinity Capture-MS
NGL2 YMR285C Protein involved in 5.8S rRNA processing genetic 1 1 1 Negative Genetic
IRA2 YOL081W GTPase-activating protein genetic 1 1 1 Negative Genetic
HYP2 YEL034W Translation elongation factor eIF-5A genetic 1 1 1 Negative Genetic
NMD5 YJR132W Karyopherin genetic 1 1 1 Positive Genetic
GSP1 YLR293C Ran GTPase physical 1 1 1 Reconstituted Complex
GET2 YER083C Subunit of the GET complex genetic 1 1 1 Synthetic Growth Defect
PDI1 YCL043C Protein disulfide isomerase genetic 1 1 1 Negative Genetic
PBR1 YNL181W Putative oxidoreductase genetic 1 1 1 Negative Genetic
SGS1 YMR190C RecQ family nucleolar DNA helicase genetic 1 1 1 Positive Genetic
HUL5 YGL141W Multiubiquitin chain assembly factor (E4) genetic 1 1 1 Negative Genetic
KRI1 YNL308C Essential nucleolar protein required for 40S ribosome biogenesis genetic 1 1 1 Positive Genetic
OMS1 YDR316W Protein integral to the mitochondrial membrane genetic 1 1 1 Negative Genetic
NOP53 YPL146C Nucleolar protein physical 1 1 1 Affinity Capture-MS
NUP2 YLR335W Nucleoporin involved in nucleocytoplasmic transport genetic 1 1 1 Positive Genetic
TPK3 YKL166C cAMP-dependent protein kinase catalytic subunit genetic 1 1 1 Phenotypic Suppression
MCK1 YNL307C Dual-specificity ser/thr and tyrosine protein kinase genetic 1 1 1 Dosage Growth Defect
EAP1 YKL204W eIF4E-associated protein, competes with eIF4G for binding to eIF4E genetic 1 1 1 Positive Genetic
PMI40 YER003C Mannose-6-phosphate isomerase genetic 1 1 1 Negative Genetic
GCR2 YNL199C Transcriptional activator of genes involved in glycolysis genetic 1 1 1 Negative Genetic
GCY1 YOR120W Glycerol dehydrogenase genetic 1 1 1 Negative Genetic
CDC7 YDL017W S/T kinase and catalytic subunit of DDK (Dbf4-dependent kinase) genetic 1 1 1 Synthetic Growth Defect
NUP133 YKR082W Subunit of Nup84p subcomplex of nuclear pore complex (NPC) genetic 1 1 1 Positive Genetic
SNF4 YGL115W Activating gamma subunit of the AMP-activated Snf1p kinase complex genetic 1 1 1 Negative Genetic
DHR2 YKL078W Predominantly nucleolar DEAH-box ATP-dependent RNA helicase genetic 1 1 1 Positive Genetic
FMP48 YGR052W Putative protein of unknown function genetic 1 1 1 Negative Genetic
AFT1 YGL071W Transcription factor involved in iron utilization and homeostasis genetic 1 1 1 Negative Genetic
GTR1 YML121W Subunit of a TORC1-stimulating GTPase and the EGO/GSE complex genetic 1 1 1 Negative Genetic
MSN5 YDR335W Karyopherin genetic 1 1 1 Negative Genetic
NUP57 YGR119C FG-nucleoporin component of central core of the nuclear pore complex genetic 1 1 1 Positive Genetic
RPS26A YGL189C Protein component of the small (40S) ribosomal subunit genetic 1 1 1 Negative Genetic
RMD1 YDL001W Cytoplasmic protein required for sporulation genetic 1 1 1 Negative Genetic
GPI16 YHR188C Subunit of the glycosylphosphatidylinositol transamidase complex genetic 1 1 1 Negative Genetic
TDH2 YJR009C Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 2 genetic 1 1 1 Negative Genetic
RLP24 YLR009W Essential protein required for ribosomal large subunit biogenesis genetic 1 1 1 Positive Genetic
TSR1 YDL060W Protein required for processing of 20S pre-rRNA in the cytoplasm physical 1 1 1 Affinity Capture-MS
RPS8A YBL072C Protein component of the small (40S) ribosomal subunit genetic 1 1 1 Positive Genetic
BFR1 YOR198C Component of mRNP complexes associated with polyribosomes physical 1 1 1 Affinity Capture-RNA
SEC9 YGR009C t-SNARE protein required for secretory vesicle-plasma membrane fusion genetic 1 1 1 Negative Genetic
STU1 YBL034C Microtubule plus-end-tracking non-motor protein genetic 1 1 1 Negative Genetic
KAR2 YJL034W ATPase involved in protein import into the ER genetic 1 1 1 Positive Genetic
FUS2 YMR232W Cell fusion regulator genetic 1 1 1 Negative Genetic
MPT5 YGL178W mRNA-binding protein of the PUF family physical 1 1 1 Affinity Capture-RNA
CSG2 YBR036C Endoplasmic reticulum membrane protein genetic 1 1 1 Negative Genetic
BCH1 YMR237W Member of the ChAPs family (Chs5p-Arf1p-binding proteins) genetic 1 1 1 Negative Genetic
SEN2 YLR105C Subunit of the tRNA splicing endonuclease genetic 1 1 1 Negative Genetic
AUR1 YKL004W Phosphatidylinositol:ceramide phosphoinositol transferase genetic 1 1 1 Negative Genetic
SHE9 YDR393W Protein required for normal mitochondrial morphology genetic 1 1 1 Negative Genetic
STM1 YLR150W Protein required for optimal translation under nutrient stress physical 1 1 1 Affinity Capture-MS
KAP120 YPL125W Karyopherin responsible for the nuclear import of Rpf1p genetic 1 1 1 Positive Genetic
PAN3 YKL025C Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex genetic 1 1 1 Positive Genetic
DOM34 YNL001W Protein that facilitates ribosomal subunit dissociation genetic 1 1 1 Negative Genetic
SSB2 YNL209W Cytoplasmic ATPase that is a ribosome-associated molecular chaperone physical 1 1 1 Co-purification
RPL28 YGL103W Ribosomal 60S subunit protein L28 genetic 1 1 1 Positive Genetic
TFB4 YPR056W Subunit of TFIIH complex genetic 1 1 1 Negative Genetic
TFB5 YDR079C-A Component of RNA polymerase II general transcription factor TFIIH genetic 1 1 1 Negative Genetic
MOG1 YJR074W Conserved nuclear protein that interacts with GTP-Gsp1p genetic 1 1 1 Positive Genetic
RPS14A YCR031C Protein component of the small (40S) ribosomal subunit genetic 1 1 1 Dosage Rescue
EMC6 YLL014W Member of conserved ER transmembrane complex genetic 1 1 1 Negative Genetic
HBS1 YKR084C GTPase with similarity to translation release factors genetic 1 1 1 Negative Genetic
OKP1 YGR179C Outer kinetochore protein required for accurate chromosome segregation genetic 1 1 1 Negative Genetic
AUA1 YFL010W-A Protein required for the negative regulation by ammonia of Gap1p genetic 1 1 1 Negative Genetic
SRS2 YJL092W DNA helicase and DNA-dependent ATPase genetic 1 1 1 Dosage Lethality
BSC6 YOL137W Protein of unknown function with 8 putative transmembrane segments genetic 1 1 1 Negative Genetic
TAD2 YJL035C Subunit of tRNA-specific adenosine-34 deaminase genetic 1 1 1 Negative Genetic
RIO1 YOR119C Serine kinase involved in cell cycle regulation and rDNA integrity genetic 1 1 1 Negative Genetic
HRQ1 YDR291W 3'-5' DNA helicase of the conserved RecQ family genetic 1 1 1 Positive Genetic
FCY21 YER060W Putative purine-cytosine permease genetic 1 1 1 Negative Genetic
MRP35 YNL122C Mitochondrial ribosomal protein of the large subunit genetic 1 1 1 Negative Genetic
RSM24 YDR175C Mitochondrial ribosomal protein of the small subunit genetic 1 1 1 Positive Genetic
ALD6 YPL061W Cytosolic aldehyde dehydrogenase genetic 1 1 1 Negative Genetic
CTP1 YBR291C Mitochondrial inner membrane citrate transporter physical 1 1 1 Affinity Capture-MS
TCM62 YBR044C Protein involved in assembly of the succinate dehydrogenase complex physical 1 1 1 Affinity Capture-MS
PSP2 YML017W Asn rich cytoplasmic protein that contains RGG motifs genetic 1 1 1 Negative Genetic
PKR1 YMR123W V-ATPase assembly factor genetic 1 1 1 Negative Genetic
GIS2 YNL255C Translational activator for mRNAs with internal ribosome entry sites physical 1 1 1 Affinity Capture-RNA
GLN4 YOR168W Glutamine tRNA synthetase genetic 1 1 1 Positive Genetic
JID1 YPR061C Probable Hsp40p co-chaperone genetic 1 1 1 Negative Genetic
COA3 YJL062W-A Mitochondrial protein required for cytochrome c oxidase assembly genetic 1 1 1 Negative Genetic
PSA1 YDL055C GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase) physical 1 1 1 PCA
SEH1 YGL100W Subunit of the Nup84 nuclear pore and SEACAT subcomplexes genetic 1 1 1 Negative Genetic
GPI17 YDR434W Transmembrane protein genetic 1 1 1 Negative Genetic
DPH5 YLR172C Methyltransferase required for synthesis of diphthamide genetic 1 1 1 Negative Genetic
APJ1 YNL077W Hsp40 chaperone with a role in SUMO-mediated protein degradation physical 1 1 1 Affinity Capture-MS
DEG1 YFL001W tRNA:pseudouridine synthase genetic 1 1 1 Positive Genetic
SKY1 YMR216C SR protein kinase (SRPK) genetic 1 1 1 Negative Genetic
NEJ1 YLR265C DNA end-binding protein required for nonhomologous end joining (NHEJ) genetic 1 1 1 Negative Genetic
SCH9 YHR205W AGC family protein kinase genetic 1 1 1 Negative Genetic
GPI19 YDR437W Subunit of GPI-GlcNAc transferase involved in synthesis of GlcNAc-PI genetic 1 1 1 Negative Genetic
CDC50 YCR094W Endosomal protein that interacts with phospholipid flippase Drs2p genetic 1 1 1 Negative Genetic
MET7 YOR241W Folylpolyglutamate synthetase genetic 1 1 1 Negative Genetic
MPS3 YJL019W Nuclear envelope protein physical 1 1 1 Affinity Capture-MS
KIP3 YGL216W Kinesin-related antiparallel sliding motor protein genetic 1 1 1 Negative Genetic
SLI15 YBR156C Subunit of the conserved chromosomal passenger complex (CPC) genetic 1 1 1 Negative Genetic
RER2 YBR002C Forms the dehydrodolichyl diphosphate synthase (DDS) complex with NUS1 genetic 1 1 1 Negative Genetic
LDB18 YLL049W Component of the dynactin complex genetic 1 1 1 Negative Genetic
HSP30 YCR021C Negative regulator of the H(+)-ATPase Pma1p genetic 1 1 1 Negative Genetic
RIB5 YBR256C Riboflavin synthase genetic 1 1 1 Negative Genetic
CEM1 YER061C Mitochondrial beta-keto-acyl synthase genetic 1 1 1 Negative Genetic
GPI13 YLL031C ER membrane localized phosphoryltransferase genetic 1 1 1 Negative Genetic
FCY22 YER060W-A Putative purine-cytosine permease genetic 1 1 1 Negative Genetic
SMP3 YOR149C Alpha 1,2-mannosyltransferase genetic 1 1 1 Negative Genetic
QRI1 YDL103C UDP-N-acetylglucosamine pyrophosphorylase genetic 1 1 1 Negative Genetic
RPL33A YPL143W Ribosomal 60S subunit protein L33A genetic 1 1 1 Positive Genetic
GPI10 YGL142C Integral membrane protein involved in GPI anchor synthesis genetic 1 1 1 Negative Genetic
MOD5 YOR274W Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase genetic 1 1 1 Negative Genetic
RPL24B YGR148C Ribosomal 60S subunit protein L24B genetic 1 1 1 Negative Genetic
GPI8 YDR331W Catalytic subunit of the ER membrane GPI transamidase complex genetic 1 1 1 Negative Genetic
TIF11 YMR260C Translation initiation factor eIF1A genetic 1 1 1 Negative Genetic
GCV1 YDR019C T subunit of the mitochondrial glycine decarboxylase complex genetic 1 1 1 Negative Genetic
SPR3 YGR059W Sporulation-specific homolog of the CDC3/10/11/12 family of genes genetic 1 1 1 Negative Genetic
SPP41 YDR464W Protein of unknown function genetic 1 1 1 Positive Genetic
TAM41 YGR046W Mitochondrial phosphatidate cytidylyltransferase (CDP-DAG synthase) genetic 1 1 1 Negative Genetic
DDI3 YFL061W Cyanamide hydratase that detoxifies cyanamide genetic 1 1 1 Negative Genetic
TMS1 YDR105C Vacuolar membrane protein of unknown function genetic 1 1 1 Negative Genetic