Standard name Systematic name Name description Description
GSP1 YLR293C Ran GTPase Ran GTPase; GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; regulated by Srm1p, Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p and Kap95p; GSP1 has a paralog, GSP2, that arose from the whole genome duplication
Visualize the interactome of GSP1
Links to external databases:
  SGD   |   KEGG   |  

Cell cycle phase and timing of transcription peak

Cell cycle phase Time (min)

CYCLoPs localization & abundance

WT1 WT2 WT3
no data no data no data

GFP localization & abundance

GFP localization GFP abundance
no data no data

GO term count

Metabolism Cell cycle Cell division Signal transduction DNA replication
GSP1 1 1 0 0 0

List of interactors

Use the search utility to find the interactor you are looking for. By clicking the column headers the table will be sorted on that column. Use shift+click to sort on multiple columns. Default sorting is on number of experiments, number of unique methods and the number of publications, and alphabetical on standard name, in that order.
Standard name Systematic name Name description Type #Experiments #Methods #Publications Evidence
KAP95 YLR347C Karyopherin beta physical 17 5 17 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Two-hybrid, Reconstituted Complex, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-Western, Co-fractionation, Reconstituted Complex, Reconstituted Complex, Reconstituted Complex, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-Western, Reconstituted Complex, Reconstituted Complex
YRB1 YDR002W Ran GTPase binding protein physical 17 4 14 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Two-hybrid, Two-hybrid, Reconstituted Complex, Reconstituted Complex, Affinity Capture-Western, Reconstituted Complex, Reconstituted Complex, Reconstituted Complex, Reconstituted Complex
CSE1 YGL238W Nuclear envelope protein that acts as a recycling factor physical 10 4 9 Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex, Reconstituted Complex, Two-hybrid, Two-hybrid, Affinity Capture-Western, Reconstituted Complex, Reconstituted Complex, Two-hybrid
CRM1 YGR218W Nuclear export factor, exportin physical 9 4 7 Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Two-hybrid, Reconstituted Complex, Reconstituted Complex, Co-purification, Reconstituted Complex
KAP123 YER110C Karyopherin beta physical 8 4 8 Two-hybrid, Reconstituted Complex, Affinity Capture-Western, Co-purification, Reconstituted Complex, Reconstituted Complex, Reconstituted Complex, Reconstituted Complex
PSE1 YMR308C Karyopherin/importin that interacts with the nuclear pore complex physical 8 3 8 Affinity Capture-Western, Reconstituted Complex, Affinity Capture-Western, Reconstituted Complex, Reconstituted Complex, Two-hybrid, Reconstituted Complex, Reconstituted Complex
SRM1 YGL097W Nucleotide exchange factor for Gsp1p physical 7 3 7 Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Reconstituted Complex, Reconstituted Complex
MOG1 YJR074W Conserved nuclear protein that interacts with GTP-Gsp1p physical 7 5 6 Affinity Capture-MS, Two-hybrid, Affinity Capture-Western, Two-hybrid, Reconstituted Complex, Affinity Capture-Western, Co-purification
KAP104 YBR017C Transportin or cytosolic karyopherin beta 2 physical 6 3 6 Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex, Reconstituted Complex, Reconstituted Complex, Reconstituted Complex
KAP114 YGL241W Karyopherin, responsible for nuclear import of specific proteins physical 6 2 5 Reconstituted Complex, Reconstituted Complex, Reconstituted Complex, Two-hybrid, Reconstituted Complex, Reconstituted Complex
LOS1 YKL205W Nuclear pore protein physical 6 4 4 Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex, Two-hybrid, Reconstituted Complex, Affinity Capture-Western
MSN5 YDR335W Karyopherin physical 5 3 5 Reconstituted Complex, Two-hybrid, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-Western
NTF2 YER009W Nuclear envelope protein genetic 5 3 4 Dosage Rescue, Dosage Rescue, Negative Genetic, Synthetic Rescue, Synthetic Rescue
YRB2 YIL063C Protein of unknown function physical 5 2 4 Affinity Capture-Western, Affinity Capture-Western, Two-hybrid, Two-hybrid, Affinity Capture-Western
KAP120 YPL125W Karyopherin responsible for the nuclear import of Rpf1p physical 5 2 4 Reconstituted Complex, Two-hybrid, Reconstituted Complex, Two-hybrid, Reconstituted Complex
MTR10 YOR160W Nuclear import receptor physical 4 3 4 Far Western, Reconstituted Complex, Two-hybrid, Reconstituted Complex
MOG1 YJR074W Conserved nuclear protein that interacts with GTP-Gsp1p genetic 4 2 4 Dosage Rescue, Dosage Rescue, Dosage Rescue, Positive Genetic
SRM1 YGL097W Nucleotide exchange factor for Gsp1p genetic 4 2 4 Dosage Rescue, Dosage Rescue, Dosage Rescue, Negative Genetic
NUP42 YDR192C FG-nucleoporin component of central core of the nuclear pore complex physical 4 4 3 Two-hybrid, Affinity Capture-MS, Reconstituted Complex, Co-crystal Structure
CEX1 YOR112W Component of nuclear aminoacylation-dependent tRNA export pathway physical 4 2 2 Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex
DHH1 YDL160C Cytoplasmic DEAD-box helicase and mRNA decapping activator physical 3 2 3 Affinity Capture-RNA, Affinity Capture-MS, Affinity Capture-RNA
NMD5 YJR132W Karyopherin physical 3 2 3 Reconstituted Complex, Affinity Capture-Western, Reconstituted Complex
NUP116 YMR047C FG-nucleoporin component of central core of the nuclear pore complex genetic 3 3 2 Dosage Rescue, Phenotypic Suppression, Negative Genetic
RNA1 YMR235C GTPase activating protein (GAP) for Gsp1p physical 3 2 2 Affinity Capture-MS, Affinity Capture-MS, Biochemical Activity
YRB2 YIL063C Protein of unknown function genetic 3 2 2 Dosage Rescue, Synthetic Rescue, Synthetic Rescue
UTP8 YGR128C Nucleolar protein required for export of tRNAs from the nucleus physical 3 2 2 Affinity Capture-Western, Reconstituted Complex, Affinity Capture-Western
PHO85 YPL031C Cyclin-dependent kinase physical 3 2 2 Biochemical Activity, Biochemical Activity, Reconstituted Complex
YRB30 YGL164C RanGTP-binding protein physical 3 3 1 Two-hybrid, Affinity Capture-Western, Reconstituted Complex
NSP1 YJL041W FG-nucleoporin component of central core of the nuclear pore complex physical 2 2 2 Affinity Capture-Western, Reconstituted Complex
NTF2 YER009W Nuclear envelope protein physical 2 2 2 Affinity Capture-Western, Reconstituted Complex
NUP159 YIL115C FG-nucleoporin component of central core of the nuclear pore complex physical 2 2 2 Two-hybrid, Reconstituted Complex
NUP100 YKL068W FG-nucleoporin component of central core of the nuclear pore complex genetic 2 2 2 Phenotypic Suppression, Negative Genetic
NUP145 YGL092W Essential protein with distinct roles in two nuclear pore subcomplexes genetic 2 2 2 Phenotypic Suppression, Negative Genetic
SRP1 YNL189W Karyopherin alpha homolog physical 2 1 2 Reconstituted Complex, Reconstituted Complex
RPN10 YHR200W Proteasome polyubiquitin receptor physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
RPN11 YFR004W Metalloprotease subunit of 19S regulatory particle physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
SPC105 YGL093W Subunit of a kinetochore-microtubule binding complex genetic 2 1 2 Negative Genetic, Negative Genetic
OKP1 YGR179C Outer kinetochore protein required for accurate chromosome segregation genetic 2 1 2 Negative Genetic, Negative Genetic
POM34 YLR018C Subunit of the transmembrane ring of the nuclear pore complex (NPC) genetic 2 1 2 Negative Genetic, Negative Genetic
NUP188 YML103C Subunit of the inner ring of the nuclear pore complex (NPC) genetic 2 1 2 Negative Genetic, Negative Genetic
CDC60 YPL160W Cytosolic leucyl tRNA synthetase genetic 2 1 2 Negative Genetic, Negative Genetic
COP1 YDL145C Alpha subunit of COPI vesicle coatomer complex genetic 2 2 1 Negative Genetic, Positive Genetic
SIR4 YDR227W SIR protein involved in assembly of silent chromatin domains physical 2 2 1 Reconstituted Complex, Affinity Capture-Western
MOB2 YFL034C-B Activator of Cbk1p kinase genetic 2 2 1 Positive Genetic, Negative Genetic
BUD5 YCR038C GTP/GDP exchange factor for Rsr1p (Bud1p) physical 2 2 1 Affinity Capture-MS, Reconstituted Complex
NUP85 YJR042W Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC) physical 2 1 1 Affinity Capture-MS, Affinity Capture-MS
FKH1 YIL131C Forkhead family transcription factor regulation 1 1 1 ChEC-Seq experiments
SFP1 YLR403W Regulates transcription of ribosomal protein and biogenesis genes regulation 1 1 1 DNA to cDNA expression microarray evidence
GCN5 YGR252W Catalytic subunit of ADA and SAGA histone acetyltransferase complexes regulation 1 1 1 chromatin immunoprecipitation-chip evidence
SPT10 YJL127C Histone H3 acetylase with a role in transcriptional regulation regulation 1 1 1 DNA to cDNA expression microarray evidence
FKH2 YNL068C Forkhead family transcription factor regulation 1 1 1 ChEC-Seq experiments
GCR1 YPL075W Transcriptional activator of genes involved in glycolysis regulation 1 1 1 DNA to cDNA expression microarray evidence
RAP1 YNL216W Essential DNA-binding transcription regulator that binds many loci regulation 1 1 1 DNA to cDNA expression microarray evidence
STP1 YDR463W Transcription factor regulation 1 1 1 chromatin immunoprecipitation-chip evidence
ABF1 YKL112W DNA binding protein with possible chromatin-reorganizing activity regulation 1 1 1 chromatin immunoprecipitation-chip evidence
REB1 YBR049C RNA polymerase I enhancer binding protein regulation 1 1 1 DNA to cDNA expression microarray evidence
CST6 YIL036W Basic leucine zipper (bZIP) transcription factor from ATF/CREB family regulation 1 1 1 DNA to cDNA expression microarray evidence
SIN3 YOL004W Component of both the Rpd3S and Rpd3L histone deacetylase complexes regulation 1 1 1 DNA to cDNA expression microarray evidence
CTF19 YPL018W Outer kinetochore protein, needed for accurate chromosome segregation genetic 1 1 1 Negative Genetic
DSN1 YIR010W Essential component of the outer kinetochore MIND complex genetic 1 1 1 Negative Genetic
CKB2 YOR039W Beta' regulatory subunit of casein kinase 2 (CK2) genetic 1 1 1 Negative Genetic
ERB1 YMR049C Constituent of 66S pre-ribosomal particles genetic 1 1 1 Negative Genetic
DED1 YOR204W ATP-dependent DEAD-box RNA helicase with strand-annealing activity physical 1 1 1 Reconstituted Complex
DIS3 YOL021C Exosome core complex catalytic subunit physical 1 1 1 Reconstituted Complex
DIS3 YOL021C Exosome core complex catalytic subunit genetic 1 1 1 Dosage Rescue
GCD1 YOR260W Gamma subunit of the translation initiation factor eIF2B genetic 1 1 1 Negative Genetic
ACT1 YFL039C Actin, structural protein in cytoskeleton genetic 1 1 1 Positive Genetic
CFT1 YDR301W RNA-binding subunit of the mRNA cleavage and polyadenylation factor genetic 1 1 1 Negative Genetic
ACC1 YNR016C Acetyl-CoA carboxylase, biotin containing enzyme genetic 1 1 1 Negative Genetic
APC1 YNL172W Largest subunit of the Anaphase-Promoting Complex/Cyclosome genetic 1 1 1 Negative Genetic
ARP3 YJR065C Essential component of the Arp2/3 complex genetic 1 1 1 Negative Genetic
ASC1 YMR116C G-protein beta subunit and guanine dissociation inhibitor for Gpa2p physical 1 1 1 Affinity Capture-MS
CCL1 YPR025C Cyclin partner of the cyclin-dependent kinase (CDK) Kin28p genetic 1 1 1 Negative Genetic
CDC10 YCR002C Component of the septin ring, required for cytokinesis genetic 1 1 1 Negative Genetic
CDC11 YJR076C Component of the septin ring that is required for cytokinesis genetic 1 1 1 Negative Genetic
CDC36 YDL165W Component of the CCR4-NOT core complex, involved in mRNA decapping genetic 1 1 1 Negative Genetic
CEG1 YGL130W Guanylyltransferase involved in mRNA 5' capping genetic 1 1 1 Negative Genetic
AVO1 YOL078W Component of the membrane-bound TORC2 complex genetic 1 1 1 Negative Genetic
DOA1 YKL213C WD-repeat protein involved in ubiquitin-mediated protein degradation physical 1 1 1 Affinity Capture-MS
CDC23 YHR166C Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) genetic 1 1 1 Positive Genetic
ECM1 YAL059W Pre-ribosomal factor involved in 60S ribosomal protein subunit export genetic 1 1 1 Negative Genetic
ENP1 YBR247C Protein associated with U3 and U14 snoRNAs genetic 1 1 1 Negative Genetic
ERO1 YML130C Thiol oxidase required for oxidative protein folding in the ER genetic 1 1 1 Negative Genetic
FIP1 YJR093C Subunit of cleavage polyadenylation factor (CPF) genetic 1 1 1 Negative Genetic
FRS1 YLR060W Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase genetic 1 1 1 Negative Genetic
ESP1 YGR098C Separase/separin, a caspase-like cysteine protease genetic 1 1 1 Negative Genetic
CSE2 YNR010W Subunit of the RNA polymerase II mediator complex genetic 1 1 1 Negative Genetic
ADA2 YDR448W Transcription coactivator genetic 1 1 1 Negative Genetic
CMD1 YBR109C Calmodulin genetic 1 1 1 Negative Genetic
DIG1 YPL049C MAP kinase-responsive inhibitor of the Ste12p transcription factor genetic 1 1 1 Negative Genetic
BLM10 YFL007W Proteasome activator physical 1 1 1 Reconstituted Complex
GLE2 YER107C RNA export factor associated with the nuclear pore complex (NPC) genetic 1 1 1 Negative Genetic
DAM1 YGR113W Essential subunit of the Dam1 complex (aka DASH complex) genetic 1 1 1 Negative Genetic
CSE1 YGL238W Nuclear envelope protein that acts as a recycling factor genetic 1 1 1 Negative Genetic
ABF2 YMR072W Mitochondrial DNA-binding protein genetic 1 1 1 Negative Genetic
CCT8 YJL008C Subunit of the cytosolic chaperonin Cct ring complex genetic 1 1 1 Negative Genetic
GCN5 YGR252W Catalytic subunit of ADA and SAGA histone acetyltransferase complexes physical 1 1 1 Affinity Capture-MS
CCT7 YJL111W Subunit of the cytosolic chaperonin Cct ring complex genetic 1 1 1 Negative Genetic
BCK1 YJL095W MAPKKK acting in the protein kinase C signaling pathway genetic 1 1 1 Positive Genetic
DIM1 YPL266W Essential 18S rRNA dimethylase (dimethyladenosine transferase) genetic 1 1 1 Negative Genetic
CRZ1 YNL027W Transcription factor, activates transcription of stress response genes genetic 1 1 1 Negative Genetic
CAF20 YOR276W Phosphoprotein of the mRNA cap-binding complex physical 1 1 1 Affinity Capture-MS
AAC3 YBR085W Mitochondrial inner membrane ADP/ATP translocator genetic 1 1 1 Negative Genetic
GSP2 YOR185C GTP binding protein (mammalian Ranp homolog) physical 1 1 1 Affinity Capture-MS
GSP2 YOR185C GTP binding protein (mammalian Ranp homolog) genetic 1 1 1 Dosage Rescue
YRB1 YDR002W Ran GTPase binding protein genetic 1 1 1 Negative Genetic
RNA1 YMR235C GTPase activating protein (GAP) for Gsp1p genetic 1 1 1 Negative Genetic
NUS1 YDL193W Forms dehydrodolichyl diphosphate synthase complex with RER2 or SRT1 physical 1 1 1 Affinity Capture-MS
SPT2 YER161C Protein involved in negative regulation of transcription physical 1 1 1 Affinity Capture-MS
HOM3 YER052C Aspartate kinase (L-aspartate 4-P-transferase) physical 1 1 1 Affinity Capture-MS
SSA1 YAL005C ATPase involved in protein folding and NLS-directed nuclear transport physical 1 1 1 Affinity Capture-MS
NUP60 YAR002W FG-nucleoporin component of central core of the nuclear pore complex physical 1 1 1 Affinity Capture-Western
NUP60 YAR002W FG-nucleoporin component of central core of the nuclear pore complex genetic 1 1 1 Negative Genetic
RRP12 YPL012W Protein required for export of the ribosomal subunits physical 1 1 1 Reconstituted Complex
SRP1 YNL189W Karyopherin alpha homolog genetic 1 1 1 Negative Genetic
KAP123 YER110C Karyopherin beta genetic 1 1 1 Negative Genetic
SHE1 YBL031W Mitotic spindle protein physical 1 1 1 Two-hybrid
SHE1 YBL031W Mitotic spindle protein genetic 1 1 1 Negative Genetic
SEC3 YER008C Subunit of the exocyst complex physical 1 1 1 Two-hybrid
RTR1 YER139C Dual specificity protein phosphatase physical 1 1 1 Two-hybrid
GTA1 YEL043W Protein of unknown function physical 1 1 1 Two-hybrid
RQC2 YPL009C Component of the Ribosome Quality Control (RQC) complex physical 1 1 1 Two-hybrid
YBR225W YBR225W Putative protein of unknown function physical 1 1 1 Two-hybrid
PSE1 YMR308C Karyopherin/importin that interacts with the nuclear pore complex genetic 1 1 1 Negative Genetic
NUP2 YLR335W Nucleoporin involved in nucleocytoplasmic transport physical 1 1 1 Two-hybrid
KSP1 YHR082C Serine/threonine protein kinase physical 1 1 1 Biochemical Activity
STE20 YHL007C Cdc42p-activated signal transducing kinase physical 1 1 1 Biochemical Activity
YCK1 YHR135C Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform physical 1 1 1 Biochemical Activity
SKP1 YDR328C Evolutionarily conserved kinetochore protein physical 1 1 1 Affinity Capture-MS
PAB1 YER165W Poly(A) binding protein physical 1 1 1 Reconstituted Complex
PAB1 YER165W Poly(A) binding protein genetic 1 1 1 Negative Genetic
SIR4 YDR227W SIR protein involved in assembly of silent chromatin domains genetic 1 1 1 Synthetic Growth Defect
SSA4 YER103W Heat shock protein that is highly induced upon stress physical 1 1 1 Reconstituted Complex
PRP43 YGL120C RNA helicase in the DEAH-box family physical 1 1 1 Affinity Capture-MS
RPN1 YHR027C Non-ATPase base subunit of the 19S RP of the 26S proteasome physical 1 1 1 Affinity Capture-MS
RPT5 YOR117W ATPase of the 19S regulatory particle of the 26S proteasome physical 1 1 1 Affinity Capture-MS
UTP9 YHR196W Nucleolar protein physical 1 1 1 Affinity Capture-Western
UTP9 YHR196W Nucleolar protein genetic 1 1 1 Positive Genetic
NAB2 YGL122C Nuclear polyadenylated RNA-binding protein physical 1 1 1 Affinity Capture-RNA
NAB2 YGL122C Nuclear polyadenylated RNA-binding protein genetic 1 1 1 Negative Genetic
SXM1 YDR395W Nuclear transport factor (karyopherin) physical 1 1 1 Reconstituted Complex
KAP122 YGL016W Karyopherin beta physical 1 1 1 Reconstituted Complex
SPB4 YFL002C Putative ATP-dependent RNA helicase physical 1 1 1 Affinity Capture-MS
SSM4 YIL030C Membrane-embedded ubiquitin-protein ligase and retrotranslocase physical 1 1 1 Protein-peptide
SLF1 YDR515W RNA binding protein that associates with polysomes physical 1 1 1 Affinity Capture-RNA
SRO9 YCL037C Cytoplasmic RNA-binding protein physical 1 1 1 Affinity Capture-RNA
SBP1 YHL034C Protein that binds eIF4G and has a role in repression of translation physical 1 1 1 Affinity Capture-RNA
PUF3 YLL013C Protein of the mitochondrial outer surface physical 1 1 1 Affinity Capture-RNA
UBP13 YBL067C Ubiquitin-specific protease that cleaves Ub-protein fusions physical 1 1 1 Affinity Capture-MS
SPT10 YJL127C Histone H3 acetylase with a role in transcriptional regulation physical 1 1 1 Affinity Capture-MS
RPS26A YGL189C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Reconstituted Complex
RPS26B YER131W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Reconstituted Complex
TSR2 YLR435W Protein with a potential role in pre-rRNA processing physical 1 1 1 Reconstituted Complex
SRP54 YPR088C Signal recognition particle (SRP) subunit (homolog of mammalian SRP54) physical 1 1 1 Co-purification
SSB2 YNL209W Cytoplasmic ATPase that is a ribosome-associated molecular chaperone physical 1 1 1 Co-purification
SLX9 YGR081C Protein required for pre-rRNA processing physical 1 1 1 Reconstituted Complex
SSB1 YDL229W Cytoplasmic ATPase that is a ribosome-associated molecular chaperone physical 1 1 1 Reconstituted Complex
RIO2 YNL207W Essential serine kinase involved in the processing of 20S pre-rRNA physical 1 1 1 Reconstituted Complex
LTV1 YKL143W Subunit of the EGO/GSE complex physical 1 1 1 Reconstituted Complex
MMS22 YLR320W Subunit of E3 ubiquitin ligase complex involved in replication repair physical 1 1 1 Affinity Capture-MS
HSP104 YLL026W Disaggregase physical 1 1 1 Affinity Capture-MS
PUF4 YGL014W Member of the PUF protein family physical 1 1 1 Affinity Capture-RNA
PUF4 YGL014W Member of the PUF protein family genetic 1 1 1 Negative Genetic
MPT5 YGL178W mRNA-binding protein of the PUF family physical 1 1 1 Affinity Capture-RNA
RET1 YOR207C Second-largest subunit of RNA polymerase III physical 1 1 1 Affinity Capture-MS
TFC6 YDR362C Subunit of RNA polymerase III transcription initiation factor complex physical 1 1 1 Affinity Capture-MS
RPS0A YGR214W Ribosomal 40S subunit protein S0A physical 1 1 1 Affinity Capture-MS
RPS1A YLR441C Ribosomal protein of the small (40S) subunit physical 1 1 1 Affinity Capture-MS
RPS2 YGL123W Protein component of the small (40S) subunit physical 1 1 1 Affinity Capture-MS
RPS6A YPL090C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS7A YOR096W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS12 YOR369C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS18A YDR450W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS20 YHL015W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS15 YOL040C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS22A YJL190C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS27A YKL156W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS3 YNL178W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS14A YCR031C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
MTR4 YJL050W RNA duplex-sensing translocase genetic 1 1 1 Dosage Rescue
PDE2 YOR360C High-affinity cyclic AMP phosphodiesterase genetic 1 1 1 Dosage Rescue
RRP4 YHR069C Exosome non-catalytic core component genetic 1 1 1 Dosage Rescue
MEC1 YBR136W Genome integrity checkpoint protein and PI kinase superfamily member genetic 1 1 1 Synthetic Rescue
NRD1 YNL251C RNA-binding subunit of Nrd1 complex genetic 1 1 1 Synthetic Growth Defect
HYP2 YEL034W Translation elongation factor eIF-5A genetic 1 1 1 Positive Genetic
MED6 YHR058C Subunit of the RNA polymerase II mediator complex genetic 1 1 1 Positive Genetic
RPL10 YLR075W Ribosomal 60S subunit protein L10 genetic 1 1 1 Positive Genetic
KRI1 YNL308C Essential nucleolar protein required for 40S ribosome biogenesis genetic 1 1 1 Positive Genetic
LAS17 YOR181W Actin assembly factor genetic 1 1 1 Positive Genetic
RPB10 YOR210W RNA polymerase subunit ABC10-beta genetic 1 1 1 Positive Genetic
SGV1 YPR161C Cyclin (Bur2p)-dependent protein kinase genetic 1 1 1 Positive Genetic
RXT2 YBR095C Component of the histone deacetylase Rpd3L complex genetic 1 1 1 Positive Genetic
VMA3 YEL027W Proteolipid subunit c of the V0 domain of vacuolar H(+)-ATPase genetic 1 1 1 Positive Genetic
VMA8 YEL051W Subunit D of the V1 peripheral membrane domain of V-ATPase genetic 1 1 1 Positive Genetic
VPS29 YHR012W Subunit of the membrane-associated retromer complex genetic 1 1 1 Positive Genetic
SLT2 YHR030C Serine/threonine MAP kinase genetic 1 1 1 Positive Genetic
SFB3 YHR098C Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat genetic 1 1 1 Positive Genetic
SET1 YHR119W Histone methyltransferase, subunit of the COMPASS (Set1C) complex genetic 1 1 1 Positive Genetic
SCH9 YHR205W AGC family protein kinase genetic 1 1 1 Positive Genetic
SDS3 YIL084C Component of the Rpd3L histone deacetylase complex genetic 1 1 1 Positive Genetic
SSL1 YLR005W Subunit of the core form of RNA polymerase transcription factor TFIIH genetic 1 1 1 Positive Genetic
RGR1 YLR071C Subunit of the RNA polymerase II mediator complex genetic 1 1 1 Positive Genetic
SLA2 YNL243W Adaptor protein that links actin to clathrin and endocytosis genetic 1 1 1 Positive Genetic
MTW1 YAL034W-A Essential component of the MIND kinetochore complex genetic 1 1 1 Negative Genetic
STU1 YBL034C Microtubule plus-end-tracking non-motor protein genetic 1 1 1 Negative Genetic
SLI15 YBR156C Subunit of the conserved chromosomal passenger complex (CPC) genetic 1 1 1 Negative Genetic
RSC6 YCR052W Component of the RSC chromatin remodeling complex genetic 1 1 1 Negative Genetic
MPS1 YDL028C Dual-specificity kinase genetic 1 1 1 Negative Genetic
PRP11 YDL043C Subunit of the SF3a splicing factor complex genetic 1 1 1 Negative Genetic
TSR1 YDL060W Protein required for processing of 20S pre-rRNA in the cytoplasm genetic 1 1 1 Negative Genetic
SUB2 YDL084W Component of the TREX complex required for nuclear mRNA export genetic 1 1 1 Negative Genetic
PSF1 YDR013W Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p) genetic 1 1 1 Negative Genetic
HEM13 YDR044W Coproporphyrinogen III oxidase genetic 1 1 1 Negative Genetic
TPI1 YDR050C Triose phosphate isomerase, abundant glycolytic enzyme genetic 1 1 1 Negative Genetic
SSS1 YDR086C Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) genetic 1 1 1 Negative Genetic
TAF12 YDR145W Subunit (61/68 kDa) of TFIID and SAGA complexes genetic 1 1 1 Negative Genetic
SEC1 YDR164C Sm-like protein involved in docking and fusion of exocytic vesicles genetic 1 1 1 Negative Genetic
SUP35 YDR172W Translation termination factor eRF3 genetic 1 1 1 Negative Genetic
UBC1 YDR177W Ubiquitin-conjugating enzyme genetic 1 1 1 Negative Genetic
SLY1 YDR189W Hydrophilic protein involved in ER/Golgi vesicle trafficking genetic 1 1 1 Negative Genetic
NCB2 YDR397C Subunit of a heterodimeric NC2 transcription regulator complex genetic 1 1 1 Negative Genetic
UTP6 YDR449C Nucleolar protein genetic 1 1 1 Negative Genetic
GUK1 YDR454C Guanylate kinase genetic 1 1 1 Negative Genetic
SEC20 YDR498C Membrane glycoprotein v-SNARE genetic 1 1 1 Negative Genetic
SMT3 YDR510W Ubiquitin-like protein of the SUMO family genetic 1 1 1 Negative Genetic
PMI40 YER003C Mannose-6-phosphate isomerase genetic 1 1 1 Negative Genetic
SPC25 YER018C Component of the kinetochore-associated Ndc80 complex genetic 1 1 1 Negative Genetic
RSP5 YER125W NEDD4 family E3 ubiquitin ligase genetic 1 1 1 Negative Genetic
SMC2 YFR031C Subunit of the condensin complex genetic 1 1 1 Negative Genetic
PRE4 YFR050C Beta 7 subunit of the 20S proteasome genetic 1 1 1 Negative Genetic
SCL1 YGL011C Alpha 1 subunit of the 20S proteasome genetic 1 1 1 Negative Genetic
RPT6 YGL048C ATPase of the 19S regulatory particle of the 26S proteasome genetic 1 1 1 Negative Genetic
OLE1 YGL055W Delta(9) fatty acid desaturase genetic 1 1 1 Negative Genetic
SEC27 YGL137W Essential beta'-coat protein of the COPI coatomer genetic 1 1 1 Negative Genetic
TFG2 YGR005C TFIIF (Transcription Factor II) middle subunit genetic 1 1 1 Negative Genetic
PRP18 YGR006W Splicing factor and component of snRNP U5 genetic 1 1 1 Negative Genetic
NUP57 YGR119C FG-nucleoporin component of central core of the nuclear pore complex genetic 1 1 1 Negative Genetic
YIP1 YGR172C Integral membrane protein genetic 1 1 1 Negative Genetic
THS1 YIL078W Threonyl-tRNA synthetase genetic 1 1 1 Negative Genetic
STS1 YIR011C Protein required for localizing proteasomes to the nucleus genetic 1 1 1 Negative Genetic
RFA3 YJL173C Subunit of heterotrimeric Replication Protein A (RPA) genetic 1 1 1 Negative Genetic
SPC42 YKL042W Central plaque component of spindle pole body (SPB) genetic 1 1 1 Negative Genetic
MPE1 YKL059C Subunit of CPF cleavage and polyadenylation factor and E3 Ub-ligase genetic 1 1 1 Negative Genetic
MIA40 YKL195W Import and assembly protein in mitochondrial intermembrane space genetic 1 1 1 Negative Genetic
MED11 YMR112C Subunit of the RNA polymerase II mediator complex genetic 1 1 1 Negative Genetic
SPC24 YMR117C Component of the kinetochore-associated Ndc80 complex genetic 1 1 1 Negative Genetic
TAF9 YMR236W Subunit (17 kDa) of TFIID and SAGA complexes genetic 1 1 1 Negative Genetic
RIA1 YNL163C Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis genetic 1 1 1 Negative Genetic
LTO1 YNL260C Substrate-specific adaptor protein involved in apo-Rli1p maturation genetic 1 1 1 Negative Genetic
POL2 YNL262W Catalytic subunit of DNA polymerase (II) epsilon genetic 1 1 1 Negative Genetic
POP3 YNL282W Subunit of both RNase MRP and nuclear RNase P genetic 1 1 1 Negative Genetic
WRS1 YOL097C Cytoplasmic tryptophanyl-tRNA synthetase genetic 1 1 1 Negative Genetic
MED7 YOL135C Subunit of the RNA polymerase II mediator complex genetic 1 1 1 Negative Genetic
RPT4 YOR259C ATPase of the 19S regulatory particle of the 26S proteasome genetic 1 1 1 Negative Genetic
YTM1 YOR272W Ribosomal assembly factor and 66S pre-ribosomal particle constituent genetic 1 1 1 Negative Genetic
SEC16 YPL085W COPII vesicle coat protein required for ER transport vesicle budding genetic 1 1 1 Negative Genetic
MEX67 YPL169C Poly(A)RNA binding protein involved in nuclear mRNA export genetic 1 1 1 Negative Genetic
NSL1 YPL233W Essential component of the MIND kinetochore complex genetic 1 1 1 Negative Genetic
TIF6 YPR016C Constituent of 66S pre-ribosomal particles genetic 1 1 1 Negative Genetic
HTS1 YPR033C Cytoplasmic and mitochondrial histidine tRNA synthetase genetic 1 1 1 Negative Genetic
YTH1 YPR107C Essential RNA-binding component of cleavage and polyadenylation factor genetic 1 1 1 Negative Genetic
NUT2 YPR168W Subunit of the RNA polymerase II mediator complex genetic 1 1 1 Negative Genetic
SEC23 YPR181C GTPase-activating protein, stimulates the GTPase activity of Sar1p genetic 1 1 1 Negative Genetic
SPO7 YAL009W Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme genetic 1 1 1 Negative Genetic
PAU8 YAL068C Protein of unknown function genetic 1 1 1 Negative Genetic
YAR029W YAR029W Member of DUP240 gene family but contains no transmembrane domains genetic 1 1 1 Negative Genetic
PHO11 YAR071W One of three repressible acid phosphatases genetic 1 1 1 Negative Genetic
HIR1 YBL008W Subunit of the HIR complex genetic 1 1 1 Negative Genetic
PRS4 YBL068W 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP genetic 1 1 1 Negative Genetic
NUP170 YBL079W Subunit of inner ring of nuclear pore complex (NPC) genetic 1 1 1 Negative Genetic
TSC3 YBR058C-A Protein that stimulates the activity of serine palmitoyltransferase genetic 1 1 1 Negative Genetic
YBR062C YBR062C Protein of unknown function that interacts with Msb2p genetic 1 1 1 Negative Genetic
SUS1 YBR111W-A Global transcriptional regulator, component of SAGA, TREX-2 complexes genetic 1 1 1 Negative Genetic
SEC66 YBR171W Non-essential subunit of Sec63 complex genetic 1 1 1 Negative Genetic
LDB16 YCL005W Protein involved in lipid droplet (LD) assembly genetic 1 1 1 Negative Genetic
MIC10 YCL057C-A Conserved component of the MICOS complex genetic 1 1 1 Negative Genetic
YCR095W-A YCR095W-A Putative protein of unknown function genetic 1 1 1 Negative Genetic
NUP84 YDL116W Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC) genetic 1 1 1 Negative Genetic
YDL199C YDL199C Putative transporter genetic 1 1 1 Negative Genetic
SHS1 YDL225W Component of the septin ring that is required for cytokinesis genetic 1 1 1 Negative Genetic
YDR061W YDR061W Protein with similarity to ABC transporter family members genetic 1 1 1 Negative Genetic
MRPL1 YDR116C Mitochondrial ribosomal protein of the large subunit genetic 1 1 1 Negative Genetic
YDR182W-A YDR182W-A Putative protein of unknown function genetic 1 1 1 Negative Genetic
MCM21 YDR318W Component of the kinetochore sub-complex COMA genetic 1 1 1 Negative Genetic
YFT2 YDR319C Protein needed for ER membrane biosynthesis in response to ER stress genetic 1 1 1 Negative Genetic
SEM1 YDR363W-A 19S proteasome regulatory particle lid subcomplex component genetic 1 1 1 Negative Genetic
RIP1 YEL024W Ubiquinol-cytochrome-c reductase genetic 1 1 1 Negative Genetic
RML2 YEL050C Mitochondrial ribosomal protein of the large subunit (L2) genetic 1 1 1 Negative Genetic
YEL076C YEL076C Putative protein of unknown function genetic 1 1 1 Negative Genetic
PRS2 YER099C 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase genetic 1 1 1 Negative Genetic
SWI4 YER111C DNA binding component of the SBF complex (Swi4p-Swi6p) genetic 1 1 1 Negative Genetic
UBP5 YER144C Putative ubiquitin-specific protease genetic 1 1 1 Negative Genetic
PET122 YER153C Mitochondrial translational activator specific for the COX3 mRNA genetic 1 1 1 Negative Genetic
ZNF1 YFL052W Zinc cluster transcription factor that regulates respiratory growth genetic 1 1 1 Negative Genetic
RPL24A YGL031C Ribosomal 60S subunit protein L24A genetic 1 1 1 Negative Genetic
PYC1 YGL062W Pyruvate carboxylase isoform genetic 1 1 1 Negative Genetic
SGF73 YGL066W Subunit of DUBm module of SAGA and SLIK genetic 1 1 1 Negative Genetic
SOH1 YGL127C Subunit of the RNA polymerase II mediator complex genetic 1 1 1 Negative Genetic
NUT1 YGL151W Component of the RNA polymerase II mediator complex genetic 1 1 1 Negative Genetic
HAP2 YGL237C Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex genetic 1 1 1 Negative Genetic
RPS25A YGR027C Protein component of the small (40S) ribosomal subunit genetic 1 1 1 Negative Genetic
RPL11B YGR085C Ribosomal 60S subunit protein L11B genetic 1 1 1 Negative Genetic
GTF1 YGR102C Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex genetic 1 1 1 Negative Genetic
SHY1 YGR112W Mitochondrial inner membrane protein required for complex IV assembly genetic 1 1 1 Negative Genetic
PCL5 YHR071W Cyclin genetic 1 1 1 Negative Genetic
NOT3 YIL038C Component of the CCR4-NOT core complex, involved in mRNA decapping genetic 1 1 1 Negative Genetic
PRK1 YIL095W Ser/Thr protein kinase genetic 1 1 1 Negative Genetic
YIL134C-A YIL134C-A Putative protein of unknown function genetic 1 1 1 Negative Genetic
RRD1 YIL153W Peptidyl-prolyl cis/trans-isomerase genetic 1 1 1 Negative Genetic
YJL136W-A YJL136W-A Putative protein of unknown function genetic 1 1 1 Negative Genetic
ROQ1 YJL144W Ub-ligase substrate-specificity factor genetic 1 1 1 Negative Genetic
IMA5 YJL216C Alpha-glucosidase genetic 1 1 1 Negative Genetic
LIA1 YJR070C Deoxyhypusine hydroxylase genetic 1 1 1 Negative Genetic
IML1 YJR138W GTPase-activating protein (GAP) subunit of the Iml1p/SEACIT complex genetic 1 1 1 Negative Genetic
TMA19 YKL056C Protein that associates with ribosomes genetic 1 1 1 Negative Genetic
MIC60 YKR016W Component of the MICOS complex genetic 1 1 1 Negative Genetic
NUP133 YKR082W Subunit of Nup84p subcomplex of nuclear pore complex (NPC) genetic 1 1 1 Negative Genetic
VBA5 YKR105C Plasma membrane protein of the Major Facilitator Superfamily (MFS) genetic 1 1 1 Negative Genetic
KRR1 YCL059C Nucleolar protein required for rRNA synthesis and ribosomal assembly genetic 1 1 1 Negative Genetic
SMD1 YGR074W Core Sm protein Sm D1 genetic 1 1 1 Negative Genetic
RPL6B YLR448W Ribosomal 60S subunit protein L6B genetic 1 1 1 Negative Genetic
RCF1 YML030W Cytochrome c oxidase subunit genetic 1 1 1 Negative Genetic
YML096W YML096W Putative protein with similarity to asparagine synthetases genetic 1 1 1 Negative Genetic
IMP2 YMR035W Catalytic subunit of mitochondrial inner membrane peptidase complex genetic 1 1 1 Negative Genetic
VBA1 YMR088C Permease of basic amino acids in the vacuolar membrane genetic 1 1 1 Negative Genetic
NUP53 YMR153W FG-nucleoporin component of central core of nuclear pore complex (NPC) genetic 1 1 1 Negative Genetic
RRG9 YNL213C Protein of unknown function genetic 1 1 1 Negative Genetic
SIN4 YNL236W Subunit of the RNA polymerase II mediator complex genetic 1 1 1 Negative Genetic
KEX2 YNL238W Kexin, a subtilisin-like protease (proprotein convertase) genetic 1 1 1 Negative Genetic
MAN2 YNR073C Mannitol dehydrogenase genetic 1 1 1 Negative Genetic
MDM38 YOL027C Membrane-associated mitochondrial ribosome receptor genetic 1 1 1 Negative Genetic
MSH2 YOL090W Protein that binds to DNA mismatches genetic 1 1 1 Negative Genetic
VPS21 YOR089C Endosomal Rab family GTPase genetic 1 1 1 Negative Genetic
MDM32 YOR147W Mitochondrial inner membrane protein with similarity to Mdm31p genetic 1 1 1 Negative Genetic
UAF30 YOR295W Subunit of UAF (upstream activation factor) complex genetic 1 1 1 Negative Genetic
YOR365C YOR365C Putative protein involved in sphingolipid biosynthesis genetic 1 1 1 Negative Genetic
YOR387C YOR387C Putative protein of unknown function genetic 1 1 1 Negative Genetic
MDM36 YPR083W Mitochondrial protein genetic 1 1 1 Negative Genetic
SNT309 YPR101W Member of the NineTeen Complex (NTC) genetic 1 1 1 Negative Genetic
NCE102 YPR149W Protein of unknown function genetic 1 1 1 Negative Genetic
YPR202W YPR202W Putative protein of unknown function genetic 1 1 1 Negative Genetic
POM33 YLL023C Transmembrane nucleoporin genetic 1 1 1 Negative Genetic
UBP8 YMR223W Ubiquitin-specific protease component of the SAGA acetylation complex genetic 1 1 1 Negative Genetic
SEC59 YMR013C Dolichol kinase genetic 1 1 1 Negative Genetic
IST2 YBR086C Cortical ER protein involved in ER-plasma membrane (PM) tethering genetic 1 1 1 Negative Genetic
MUD2 YKL074C Protein involved in early pre-mRNA splicing genetic 1 1 1 Negative Genetic
HTD2 YHR067W Mitochondrial 3-hydroxyacyl-thioester dehydratase genetic 1 1 1 Positive Genetic
HOG1 YLR113W Mitogen-activated protein kinase involved in osmoregulation genetic 1 1 1 Phenotypic Suppression
HRQ1 YDR291W 3'-5' DNA helicase of the conserved RecQ family genetic 1 1 1 Negative Genetic
SGS1 YMR190C RecQ family nucleolar DNA helicase genetic 1 1 1 Negative Genetic
FAR1 YJL157C CDK inhibitor and nuclear anchor physical 1 1 1 Affinity Capture-Western
ERG27 YLR100W 3-keto sterol reductase genetic 1 1 1 Negative Genetic
CCT3 YJL014W Subunit of the cytosolic chaperonin Cct ring complex genetic 1 1 1 Negative Genetic
BUD32 YGR262C Protein kinase genetic 1 1 1 Negative Genetic
GLE1 YDL207W Cytoplasmic nucleoporin required for polyadenylated mRNA export genetic 1 1 1 Negative Genetic
FRS2 YFL022C Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase physical 1 1 1 Two-hybrid
DEF1 YKL054C RNAPII degradation factor genetic 1 1 1 Negative Genetic
EXO70 YJL085W Subunit of the exocyst complex genetic 1 1 1 Negative Genetic
CDC43 YGL155W Beta subunit of geranylgeranyltransferase type I genetic 1 1 1 Negative Genetic
GPI16 YHR188C Subunit of the glycosylphosphatidylinositol transamidase complex genetic 1 1 1 Positive Genetic
ASK1 YKL052C Essential subunit of the Dam1 complex (aka DASH complex) genetic 1 1 1 Negative Genetic
APC5 YOR249C Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) genetic 1 1 1 Negative Genetic
CDC27 YBL084C Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) genetic 1 1 1 Negative Genetic
COG5 YNL051W Component of the conserved oligomeric Golgi complex genetic 1 1 1 Positive Genetic
BFR1 YOR198C Component of mRNP complexes associated with polyribosomes physical 1 1 1 Affinity Capture-RNA
GCD10 YNL062C Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p genetic 1 1 1 Negative Genetic
CBF2 YGR140W Essential kinetochore protein genetic 1 1 1 Negative Genetic
GLN3 YER040W Transcriptional activator in nitrogen catabolite repression system genetic 1 1 1 Negative Genetic
FCP1 YMR277W Carboxy-terminal domain (CTD) phosphatase genetic 1 1 1 Negative Genetic
CDC19 YAL038W Pyruvate kinase genetic 1 1 1 Negative Genetic
ARK1 YNL020C Ser/Thr protein kinase genetic 1 1 1 Negative Genetic
BET4 YJL031C Alpha subunit of Type II geranylgeranyltransferase genetic 1 1 1 Negative Genetic
ERG7 YHR072W Lanosterol synthase genetic 1 1 1 Positive Genetic
BRL1 YHR036W Essential nuclear envelope/ER integral membrane protein genetic 1 1 1 Positive Genetic
GEP7 YGL057C Protein of unknown function genetic 1 1 1 Negative Genetic
GCD14 YJL125C Subunit of tRNA (1-methyladenosine) methyltransferase genetic 1 1 1 Negative Genetic
GLN4 YOR168W Glutamine tRNA synthetase genetic 1 1 1 Negative Genetic
DEG1 YFL001W tRNA:pseudouridine synthase genetic 1 1 1 Negative Genetic
COQ2 YNR041C Para hydroxybenzoate polyprenyl transferase genetic 1 1 1 Negative Genetic
BUD16 YEL029C Putative pyridoxal kinase genetic 1 1 1 Negative Genetic
FKS3 YMR306W Protein involved in spore wall assembly genetic 1 1 1 Negative Genetic
EFM4 YIL064W Lysine methyltransferase genetic 1 1 1 Negative Genetic
ECM34 YHL043W Protein of unknown function genetic 1 1 1 Negative Genetic
GCV1 YDR019C T subunit of the mitochondrial glycine decarboxylase complex genetic 1 1 1 Negative Genetic
BCD1 YHR040W Essential protein required for the accumulation of box C/D snoRNA genetic 1 1 1 Positive Genetic
EXO5 YBR163W Mitochondrial 5'-3' exonuclease and sliding exonuclease genetic 1 1 1 Negative Genetic
ATP23 YNR020C Putative metalloprotease of the mitochondrial inner membrane genetic 1 1 1 Negative Genetic
APQ12 YIL040W Nuclear envelope/ER integral membrane protein genetic 1 1 1 Negative Genetic