Standard name Systematic name Name description Description
PRE1 YER012W Beta 4 subunit of the 20S proteasome Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
Visualize the interactome of PRE1
Links to external databases:
  SGD   |   KEGG   |  

Cell cycle phase and timing of transcription peak

Cell cycle phase Time (min)

CYCLoPs localization & abundance

WT1 WT2 WT3
no data no data no data

GFP localization & abundance

GFP localization GFP abundance
no data 21800

GO term count

Metabolism Cell cycle Cell division Signal transduction DNA replication
PRE1 3 0 0 0 0

Enzyme information

Catalyzed reactions in Yeast8

List of interactors

Use the search utility to find the interactor you are looking for. By clicking the column headers the table will be sorted on that column. Use shift+click to sort on multiple columns. Default sorting is on number of experiments, number of unique methods and the number of publications, and alphabetical on standard name, in that order.
Standard name Systematic name Name description Type #Experiments #Methods #Publications Evidence
PRE10 YOR362C Alpha 7 subunit of the 20S proteasome physical 17 3 16 Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western
RPT1 YKL145W ATPase of the 19S regulatory particle of the 26S proteasome physical 15 4 14 Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-Western, Co-purification, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-MS, Co-purification, Affinity Capture-MS, Affinity Capture-Western
PRE6 YOL038W Alpha 4 subunit of the 20S proteasome physical 15 3 14 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
PRE2 YPR103W Beta 5 subunit of the 20S proteasome physical 14 4 13 Affinity Capture-MS, Affinity Capture-MS, Two-hybrid, Two-hybrid, Affinity Capture-MS, Co-purification, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
RPN11 YFR004W Metalloprotease subunit of 19S regulatory particle physical 12 3 12 Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Co-purification, Affinity Capture-MS
PRE8 YML092C Alpha 2 subunit of the 20S proteasome physical 12 3 11 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
PRE5 YMR314W Alpha 6 subunit of the 20S proteasome physical 12 3 11 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
PUP2 YGR253C Alpha 5 subunit of the 20S proteasome physical 12 3 11 Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
PRE9 YGR135W Alpha 3 subunit of the 20S proteasome physical 12 3 11 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
SCL1 YGL011C Alpha 1 subunit of the 20S proteasome physical 11 3 10 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
PRE2 YPR103W Beta 5 subunit of the 20S proteasome genetic 11 5 9 Phenotypic Enhancement, Phenotypic Enhancement, Phenotypic Enhancement, Phenotypic Enhancement, Synthetic Growth Defect, Phenotypic Enhancement, Synthetic Growth Defect, Synthetic Rescue, Positive Genetic, Positive Genetic, Negative Genetic
RPN10 YHR200W Proteasome polyubiquitin receptor physical 11 4 9 Affinity Capture-MS, Affinity Capture-MS, Co-purification, Co-purification, Co-purification, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Reconstituted Complex
PRE4 YFR050C Beta 7 subunit of the 20S proteasome physical 10 3 10 Two-hybrid, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western
PRE3 YJL001W Beta 1 subunit of the 20S proteasome physical 10 2 10 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
PUP3 YER094C Beta 3 subunit of the 20S proteasome physical 10 2 10 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
RPN5 YDL147W Subunit of the CSN and 26S proteasome lid complexes physical 9 3 9 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex
PRE7 YBL041W Beta 6 subunit of the 20S proteasome physical 9 2 9 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
RPN12 YFR052W Subunit of the 19S regulatory particle of the 26S proteasome lid physical 8 2 8 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS
RPN8 YOR261C Essential non-ATPase regulatory subunit of the 26S proteasome physical 8 2 8 Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western
RPT6 YGL048C ATPase of the 19S regulatory particle of the 26S proteasome physical 7 3 7 Affinity Capture-Western, Affinity Capture-MS, Co-purification, Affinity Capture-MS, Co-purification, Affinity Capture-Western, Affinity Capture-MS
PUP1 YOR157C Beta 2 subunit of the 20S proteasome physical 7 2 7 Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
PRE4 YFR050C Beta 7 subunit of the 20S proteasome genetic 6 4 5 Phenotypic Enhancement, Synthetic Growth Defect, Phenotypic Enhancement, Positive Genetic, Positive Genetic, Negative Genetic
RPN1 YHR027C Non-ATPase base subunit of the 19S RP of the 26S proteasome physical 5 2 5 Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS
RPT5 YOR117W ATPase of the 19S regulatory particle of the 26S proteasome physical 5 2 5 Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
UMP1 YBR173C Chaperone required for correct maturation of the 20S proteasome physical 5 1 5 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
RPN3 YER021W Essential non-ATPase regulatory subunit of the 26S proteasome lid physical 4 2 4 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western
RPT4 YOR259C ATPase of the 19S regulatory particle of the 26S proteasome physical 4 2 4 Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS
SSA1 YAL005C ATPase involved in protein folding and NLS-directed nuclear transport physical 4 2 4 Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-MS, Affinity Capture-MS
ADD66 YKL206C Protein involved in 20S proteasome assembly physical 4 1 4 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
RAD23 YEL037C Protein with ubiquitin-like N terminus physical 4 3 3 Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-MS, Reconstituted Complex
ACT1 YFL039C Actin, structural protein in cytoskeleton genetic 4 3 2 Synthetic Haploinsufficiency, Synthetic Haploinsufficiency, Positive Genetic, Negative Genetic
BLM10 YFL007W Proteasome activator physical 3 2 3 Affinity Capture-MS, Affinity Capture-MS, Protein-peptide
PRE1 YER012W Beta 4 subunit of the 20S proteasome physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
RPN2 YIL075C Subunit of the 26S proteasome physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
RPN6 YDL097C Essential, non-ATPase regulatory subunit of the 26S proteasome lid physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
RPN7 YPR108W Essential non-ATPase regulatory subunit of the 26S proteasome physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
RPN9 YDR427W Non-ATPase regulatory subunit of the 26S proteasome physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
RPT2 YDL007W ATPase of the 19S regulatory particle of the 26S proteasome physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
RPT3 YDR394W ATPase of the 19S regulatory particle of the 26S proteasome physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
RPN13 YLR421C Subunit of the 19S regulatory particle of the 26S proteasome lid physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
UBP6 YFR010W Ubiquitin-specific protease physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
PBA1 YLR199C Protein involved in 20S proteasome assembly physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
PRE6 YOL038W Alpha 4 subunit of the 20S proteasome genetic 3 2 2 Positive Genetic, Positive Genetic, Negative Genetic
PRE3 YJL001W Beta 1 subunit of the 20S proteasome genetic 3 2 2 Synthetic Growth Defect, Positive Genetic, Positive Genetic
PRE10 YOR362C Alpha 7 subunit of the 20S proteasome genetic 3 2 2 Positive Genetic, Positive Genetic, Negative Genetic
SCL1 YGL011C Alpha 1 subunit of the 20S proteasome genetic 3 2 2 Positive Genetic, Positive Genetic, Negative Genetic
PRE7 YBL041W Beta 6 subunit of the 20S proteasome genetic 3 2 2 Positive Genetic, Positive Genetic, Negative Genetic
PUP1 YOR157C Beta 2 subunit of the 20S proteasome genetic 3 2 2 Positive Genetic, Positive Genetic, Negative Genetic
RPN10 YHR200W Proteasome polyubiquitin receptor genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
PRE9 YGR135W Alpha 3 subunit of the 20S proteasome genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
RPN6 YDL097C Essential, non-ATPase regulatory subunit of the 26S proteasome lid genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
RPN4 YDL020C Transcription factor that stimulates expression of proteasome genes regulation 2 2 2 chromatin immunoprecipitation-chip evidence, DNA to cDNA expression microarray evidence
CDC48 YDL126C AAA ATPase with protein-unfoldase activity physical 2 2 2 Affinity Capture-MS, Affinity Capture-Western
SEM1 YDR363W-A 19S proteasome regulatory particle lid subcomplex component physical 2 2 2 Affinity Capture-Western, Affinity Capture-MS
SSB2 YNL209W Cytoplasmic ATPase that is a ribosome-associated molecular chaperone physical 2 2 2 Affinity Capture-MS, Co-purification
UBC4 YBR082C Ubiquitin-conjugating enzyme (E2) physical 2 2 2 Co-purification, Affinity Capture-Western
SEC61 YLR378C Conserved ER protein translocation channel genetic 2 2 2 Phenotypic Suppression, Negative Genetic
ECM29 YHL030W Scaffold protein physical 2 2 2 Affinity Capture-MS, Affinity Capture-Western
NAS6 YGR232W Evolutionarily conserved 19S regulatory particle assembly-chaperone physical 2 2 2 Affinity Capture-MS, Reconstituted Complex
FKH1 YIL131C Forkhead family transcription factor regulation 2 1 2 chromatin immunoprecipitation-chip evidence, chromatin immunoprecipitation-chip evidence
SSA2 YLL024C HSP70 family ATP-binding protein physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
UBI4 YLL039C Ubiquitin physical 2 1 2 Affinity Capture-Western, Affinity Capture-Western
SLY1 YDR189W Hydrophilic protein involved in ER/Golgi vesicle trafficking genetic 2 2 1 Synthetic Lethality, Phenotypic Suppression
PUP2 YGR253C Alpha 5 subunit of the 20S proteasome genetic 2 1 1 Positive Genetic, Positive Genetic
RPN8 YOR261C Essential non-ATPase regulatory subunit of the 26S proteasome genetic 2 1 1 Negative Genetic, Negative Genetic
RPT1 YKL145W ATPase of the 19S regulatory particle of the 26S proteasome genetic 2 1 1 Positive Genetic, Positive Genetic
RPT6 YGL048C ATPase of the 19S regulatory particle of the 26S proteasome genetic 2 1 1 Positive Genetic, Positive Genetic
RPN7 YPR108W Essential non-ATPase regulatory subunit of the 26S proteasome genetic 2 1 1 Positive Genetic, Positive Genetic
RPN11 YFR004W Metalloprotease subunit of 19S regulatory particle genetic 2 1 1 Positive Genetic, Positive Genetic
RPT2 YDL007W ATPase of the 19S regulatory particle of the 26S proteasome genetic 2 1 1 Positive Genetic, Positive Genetic
RPT3 YDR394W ATPase of the 19S regulatory particle of the 26S proteasome genetic 2 1 1 Negative Genetic, Negative Genetic
RPT5 YOR117W ATPase of the 19S regulatory particle of the 26S proteasome genetic 2 1 1 Positive Genetic, Positive Genetic
WSS1 YHR134W SUMO-ligase and SUMO-targeted metalloprotease physical 2 1 1 Affinity Capture-Western, Affinity Capture-Western
SFP1 YLR403W Regulates transcription of ribosomal protein and biogenesis genes regulation 1 1 1 DNA to cDNA expression microarray evidence
GCN5 YGR252W Catalytic subunit of ADA and SAGA histone acetyltransferase complexes regulation 1 1 1 chromatin immunoprecipitation-chip evidence
FKH2 YNL068C Forkhead family transcription factor regulation 1 1 1 chromatin immunoprecipitation-chip evidence
GCR1 YPL075W Transcriptional activator of genes involved in glycolysis regulation 1 1 1 DNA to cDNA expression microarray evidence
CDC73 YLR418C Component of the Paf1p complex genetic 1 1 1 Synthetic Lethality
CTR9 YOL145C Component of the Paf1p complex involved in transcription elongation physical 1 1 1 Affinity Capture-MS
LEO1 YOR123C Component of the Paf1 complex physical 1 1 1 Affinity Capture-MS
PAF1 YBR279W Component of the Paf1p complex involved in transcription elongation physical 1 1 1 Affinity Capture-MS
IKI3 YLR384C Subunit of Elongator complex genetic 1 1 1 Negative Genetic
DBP9 YLR276C DEAD-box protein required for 27S rRNA processing physical 1 1 1 Affinity Capture-MS
MES1 YGR264C Methionyl-tRNA synthetase genetic 1 1 1 Negative Genetic
BMH1 YER177W 14-3-3 protein, major isoform physical 1 1 1 Affinity Capture-MS
NSR1 YGR159C Nucleolin, nucleolar protein that binds nuclear localization sequences genetic 1 1 1 Dosage Lethality
MAK21 YDR060W Constituent of 66S pre-ribosomal particles genetic 1 1 1 Negative Genetic
LSM1 YJL124C Lsm (Like Sm) protein genetic 1 1 1 Positive Genetic
PAT1 YCR077C Deadenylation-dependent mRNA-decapping factor genetic 1 1 1 Positive Genetic
CDC48 YDL126C AAA ATPase with protein-unfoldase activity genetic 1 1 1 Negative Genetic
MED4 YOR174W Subunit of the RNA polymerase II mediator complex genetic 1 1 1 Positive Genetic
KAP95 YLR347C Karyopherin beta genetic 1 1 1 Negative Genetic
RPN12 YFR052W Subunit of the 19S regulatory particle of the 26S proteasome lid genetic 1 1 1 Negative Genetic
RPN5 YDL147W Subunit of the CSN and 26S proteasome lid complexes genetic 1 1 1 Negative Genetic
YKU80 YMR106C Subunit of telomeric Ku complex (Yku70p-Yku80p) physical 1 1 1 Affinity Capture-MS
YHR033W YHR033W Putative protein of unknown function physical 1 1 1 Affinity Capture-MS
UBC1 YDR177W Ubiquitin-conjugating enzyme physical 1 1 1 Affinity Capture-Western
RAD6 YGL058W Ubiquitin-conjugating enzyme (E2) physical 1 1 1 Affinity Capture-Western
SSA4 YER103W Heat shock protein that is highly induced upon stress physical 1 1 1 Affinity Capture-MS
TEF2 YBR118W Translational elongation factor EF-1 alpha physical 1 1 1 Affinity Capture-MS
RPL13A YDL082W Ribosomal 60S subunit protein L13A physical 1 1 1 Affinity Capture-MS
RPL13A YDL082W Ribosomal 60S subunit protein L13A genetic 1 1 1 Negative Genetic
RTF1 YGL244W Subunit of RNAPII-associated chromatin remodeling Paf1 complex physical 1 1 1 Affinity Capture-MS
SHM2 YLR058C Cytosolic serine hydroxymethyltransferase physical 1 1 1 Affinity Capture-MS
TRP5 YGL026C Tryptophan synthase physical 1 1 1 Affinity Capture-MS
URA2 YJL130C Bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase physical 1 1 1 Affinity Capture-MS
PSA1 YDL055C GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase) physical 1 1 1 Affinity Capture-MS
RPL4A YBR031W Ribosomal 60S subunit protein L4A physical 1 1 1 Affinity Capture-MS
RPL4B YDR012W Ribosomal 60S subunit protein L4B physical 1 1 1 Affinity Capture-MS
RPL2B YIL018W Ribosomal 60S subunit protein L2B physical 1 1 1 Affinity Capture-MS
RPL9A YGL147C Ribosomal 60S subunit protein L9A physical 1 1 1 Affinity Capture-MS
RPL12A YEL054C Ribosomal 60S subunit protein L12A physical 1 1 1 Affinity Capture-MS
RKR1 YMR247C RING domain E3 ubiquitin ligase physical 1 1 1 Affinity Capture-MS
RPL5 YPL131W Ribosomal 60S subunit protein L5 physical 1 1 1 Affinity Capture-MS
YLL054C YLL054C Putative protein of unknown function with similarity to Pip2p physical 1 1 1 Affinity Capture-MS
SLI15 YBR156C Subunit of the conserved chromosomal passenger complex (CPC) physical 1 1 1 Affinity Capture-MS
PUB1 YNL016W Poly (A)+ RNA-binding protein physical 1 1 1 Affinity Capture-MS
TEF1 YPR080W Translational elongation factor EF-1 alpha physical 1 1 1 Affinity Capture-Western
TOS8 YGL096W Homeodomain-containing protein and putative transcription factor physical 1 1 1 Affinity Capture-MS
SRS2 YJL092W DNA helicase and DNA-dependent ATPase physical 1 1 1 Affinity Capture-MS
YJL132W YJL132W Putative protein of unknown function physical 1 1 1 Affinity Capture-MS
PRS2 YER099C 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase physical 1 1 1 Affinity Capture-Western
RPO21 YDL140C RNA polymerase II largest subunit B220 physical 1 1 1 Affinity Capture-Western
UBI4 YLL039C Ubiquitin genetic 1 1 1 Synthetic Rescue
UBX5 YDR330W UBX domain-containing protein that interacts with Cdc48p physical 1 1 1 Affinity Capture-Western
SPB4 YFL002C Putative ATP-dependent RNA helicase physical 1 1 1 Affinity Capture-MS
SBP1 YHL034C Protein that binds eIF4G and has a role in repression of translation physical 1 1 1 Affinity Capture-RNA
YEF3 YLR249W Translation elongation factor 3 physical 1 1 1 Affinity Capture-Western
TEF4 YKL081W Gamma subunit of translational elongation factor eEF1B physical 1 1 1 Affinity Capture-Western
SIC1 YLR079W Cyclin-dependent kinase inhibitor (CKI) physical 1 1 1 Reconstituted Complex
PTC7 YHR076W Type 2C serine/threonine protein phosphatase (PP2C) physical 1 1 1 Affinity Capture-MS
SSE1 YPL106C ATPase component of heat shock protein Hsp90 chaperone complex physical 1 1 1 Affinity Capture-Western
RET1 YOR207C Second-largest subunit of RNA polymerase III physical 1 1 1 Affinity Capture-MS
RPS3 YNL178W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
SIT4 YDL047W Ceramide-activated, type 2A-related serine-threonine phosphatase genetic 1 1 1 Synthetic Lethality
ROT1 YMR200W Molecular chaperone involved in protein folding in ER genetic 1 1 1 Synthetic Growth Defect
SAR1 YPL218W ARF family GTPase genetic 1 1 1 Dosage Lethality
VMA16 YHR026W Subunit c'' of the vacuolar ATPase genetic 1 1 1 Dosage Lethality
STM1 YLR150W Protein required for optimal translation under nutrient stress genetic 1 1 1 Dosage Lethality
STE6 YKL209C Plasma membrane ATP-binding cassette (ABC) transporter genetic 1 1 1 Synthetic Growth Defect
UFE1 YOR075W t-SNARE protein required for retrograde vesicular traffic genetic 1 1 1 Phenotypic Suppression
TTI1 YKL033W Subunit of the ASTRA complex, involved in chromatin remodeling genetic 1 1 1 Negative Genetic
TRS20 YBR254C Core component of transport protein particle (TRAPP) complexes I-III genetic 1 1 1 Positive Genetic
RRP1 YDR087C Essential evolutionarily conserved nucleolar protein genetic 1 1 1 Positive Genetic
SUP35 YDR172W Translation termination factor eRF3 genetic 1 1 1 Positive Genetic
TRS23 YDR246W Core component of transport protein particle (TRAPP) complexes I-III genetic 1 1 1 Positive Genetic
TFB1 YDR311W Subunit of TFIIH and nucleotide excision repair factor 3 complexes genetic 1 1 1 Positive Genetic
SSL1 YLR005W Subunit of the core form of RNA polymerase transcription factor TFIIH genetic 1 1 1 Positive Genetic
SNT1 YCR033W Set3C histone deacetylase scaffold genetic 1 1 1 Positive Genetic
THR4 YCR053W Threonine synthase genetic 1 1 1 Positive Genetic
PTC1 YDL006W Type 2C protein phosphatase (PP2C) genetic 1 1 1 Positive Genetic
SLX5 YDL013W Subunit of the Slx5-Slx8 SUMO-targeted Ub ligase (STUbL) complex genetic 1 1 1 Positive Genetic
SGF73 YGL066W Subunit of DUBm module of SAGA and SLIK genetic 1 1 1 Positive Genetic
UPS1 YLR193C Phosphatidic acid transfer protein genetic 1 1 1 Positive Genetic
SST2 YLR452C GTPase-activating protein for Gpa1p genetic 1 1 1 Positive Genetic
SUR1 YPL057C Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit genetic 1 1 1 Positive Genetic
SEC18 YBR080C AAA ATPase and SNARE disassembly chaperone genetic 1 1 1 Negative Genetic
TAF5 YBR198C Subunit (90 kDa) of TFIID and SAGA complexes genetic 1 1 1 Negative Genetic
SSS1 YDR086C Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) genetic 1 1 1 Negative Genetic
SEC20 YDR498C Membrane glycoprotein v-SNARE genetic 1 1 1 Negative Genetic
SEC53 YFL045C Phosphomannomutase genetic 1 1 1 Negative Genetic
SAD1 YFR005C Conserved zinc-finger domain protein involved in pre-mRNA splicing genetic 1 1 1 Negative Genetic
SPT16 YGL207W Subunit of the heterodimeric FACT complex (Spt16p-Pob3p) genetic 1 1 1 Negative Genetic
PRP18 YGR006W Splicing factor and component of snRNP U5 genetic 1 1 1 Negative Genetic
TIM44 YIL022W Essential component of the TIM23 complex genetic 1 1 1 Negative Genetic
STS1 YIR011C Protein required for localizing proteasomes to the nucleus genetic 1 1 1 Negative Genetic
UGP1 YKL035W UDP-glucose pyrophosphorylase (UGPase) genetic 1 1 1 Negative Genetic
RNT1 YMR239C Nuclear dsRNA-specific ribonuclease (RNase III) genetic 1 1 1 Negative Genetic
SRV2 YNL138W CAP (cyclase-associated protein) genetic 1 1 1 Negative Genetic
SEC12 YNR026C Guanine nucleotide exchange factor (GEF) genetic 1 1 1 Negative Genetic
RPB2 YOR151C RNA polymerase II second largest subunit B150 genetic 1 1 1 Negative Genetic
SEC63 YOR254C Essential subunit of Sec63 complex genetic 1 1 1 Negative Genetic
TIM50 YPL063W Essential component of the TIM23 complex genetic 1 1 1 Negative Genetic
SEC16 YPL085W COPII vesicle coat protein required for ER transport vesicle budding genetic 1 1 1 Negative Genetic
SEC62 YPL094C Essential subunit of Sec63 complex genetic 1 1 1 Negative Genetic
PRP4 YPR178W Splicing factor genetic 1 1 1 Negative Genetic
SWD1 YAR003W Subunit of the COMPASS (Set1C) complex genetic 1 1 1 Negative Genetic
SHE1 YBL031W Mitotic spindle protein genetic 1 1 1 Negative Genetic
SEC66 YBR171W Non-essential subunit of Sec63 complex genetic 1 1 1 Negative Genetic
RIM1 YCR028C-A ssDNA-binding protein essential for mitochondrial genome maintenance genetic 1 1 1 Negative Genetic
RPN4 YDL020C Transcription factor that stimulates expression of proteasome genes genetic 1 1 1 Negative Genetic
RPL35A YDL191W Ribosomal 60S subunit protein L35A genetic 1 1 1 Negative Genetic
XRN1 YGL173C Evolutionarily-conserved 5'-3' exonuclease genetic 1 1 1 Negative Genetic
YGL235W YGL235W Putative protein of unknown function genetic 1 1 1 Negative Genetic
SNF6 YHL025W Subunit of the SWI/SNF chromatin remodeling complex genetic 1 1 1 Negative Genetic
RPS27B YHR021C Protein component of the small (40S) ribosomal subunit genetic 1 1 1 Negative Genetic
YJL206C YJL206C Putative protein of unknown function genetic 1 1 1 Negative Genetic
RPL14A YKL006W Ribosomal 60S subunit protein L14A genetic 1 1 1 Negative Genetic
VPS24 YKL041W One of four subunits of the ESCRT-III complex genetic 1 1 1 Negative Genetic
SRC1 YML034W Inner nuclear membrane protein genetic 1 1 1 Negative Genetic
YML108W YML108W Protein of unknown function genetic 1 1 1 Negative Genetic
UBX4 YMR067C UBX domain-containing protein that interacts with Cdc48p genetic 1 1 1 Negative Genetic
SWS2 YNL081C Putative mitochondrial ribosomal protein of the small subunit genetic 1 1 1 Negative Genetic
RPS28A YOR167C Protein component of the small (40S) ribosomal subunit genetic 1 1 1 Negative Genetic
SSP2 YOR242C Sporulation specific protein that localizes to the spore wall genetic 1 1 1 Negative Genetic
RPS10A YOR293W Protein component of the small (40S) ribosomal subunit genetic 1 1 1 Negative Genetic
VTS1 YOR359W Flap-structured DNA-binding and RNA-binding protein genetic 1 1 1 Negative Genetic
TGS1 YPL157W Trimethyl guanosine synthase, conserved nucleolar methyl transferase genetic 1 1 1 Negative Genetic
AAH1 YNL141W Adenine deaminase (adenine aminohydrolase) genetic 1 1 1 Positive Genetic
AAR2 YBL074C Component of the U5 snRNP complex genetic 1 1 1 Negative Genetic
ALA1 YOR335C Cytoplasmic and mitochondrial alanyl-tRNA synthetase genetic 1 1 1 Negative Genetic
NAB2 YGL122C Nuclear polyadenylated RNA-binding protein physical 1 1 1 Affinity Capture-RNA
OLA1 YBR025C P-loop ATPase with similarity to human OLA1 and bacterial YchF physical 1 1 1 Affinity Capture-Western
ACC1 YNR016C Acetyl-CoA carboxylase, biotin containing enzyme physical 1 1 1 Affinity Capture-MS
BET3 YKR068C Core component of transport protein particle (TRAPP) complexes I-III genetic 1 1 1 Positive Genetic
BMH2 YDR099W 14-3-3 protein, minor isoform physical 1 1 1 Affinity Capture-MS
ASC1 YMR116C G-protein beta subunit and guanine dissociation inhibitor for Gpa2p physical 1 1 1 Affinity Capture-MS
CDC14 YFR028C Protein phosphatase required for mitotic exit physical 1 1 1 Affinity Capture-MS
CDC28 YBR160W Cyclin-dependent kinase (CDK) catalytic subunit physical 1 1 1 Co-purification
CDC11 YJR076C Component of the septin ring that is required for cytokinesis genetic 1 1 1 Negative Genetic
BUD31 YCR063W Component of the SF3b subcomplex of the U2 snRNP genetic 1 1 1 Negative Genetic
COX5A YNL052W Subunit Va of cytochrome c oxidase genetic 1 1 1 Negative Genetic
CTF18 YMR078C Subunit of a complex with Ctf8p genetic 1 1 1 Negative Genetic
CTK1 YKL139W Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I) genetic 1 1 1 Negative Genetic
DCP2 YNL118C Catalytic subunit of Dcp1p-Dcp2p decapping enzyme complex genetic 1 1 1 Negative Genetic
DNA2 YHR164C ATP-dependent 5'-flap endonuclease and DNA helicase genetic 1 1 1 Negative Genetic
DOA1 YKL213C WD-repeat protein involved in ubiquitin-mediated protein degradation genetic 1 1 1 Phenotypic Suppression
CDC23 YHR166C Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) genetic 1 1 1 Negative Genetic
ENO1 YGR254W Enolase I, a phosphopyruvate hydratase genetic 1 1 1 Negative Genetic
ESP1 YGR098C Separase/separin, a caspase-like cysteine protease genetic 1 1 1 Negative Genetic
DHH1 YDL160C Cytoplasmic DEAD-box helicase and mRNA decapping activator physical 1 1 1 Affinity Capture-RNA
ACK1 YDL203C Protein that functions in the cell wall integrity pathway physical 1 1 1 Affinity Capture-MS
CRM1 YGR218W Nuclear export factor, exportin genetic 1 1 1 Negative Genetic
ENO2 YHR174W Enolase II, a phosphopyruvate hydratase physical 1 1 1 Affinity Capture-MS
MAK5 YBR142W Essential nucleolar protein genetic 1 1 1 Positive Genetic
MOT1 YPL082C Essential protein involved in regulation of transcription genetic 1 1 1 Negative Genetic
HSC82 YMR186W Cytoplasmic chaperone of the Hsp90 family physical 1 1 1 Affinity Capture-MS
MYO2 YOR326W Type V myosin motor involved in actin-based transport of cargos genetic 1 1 1 Negative Genetic
NBP35 YGL091C Essential cytoplasmic iron-sulfur cluster binding protein genetic 1 1 1 Negative Genetic
MIS1 YBR084W Mitochondrial C1-tetrahydrofolate synthase genetic 1 1 1 Negative Genetic
PPH22 YDL188C Catalytic subunit of protein phosphatase 2A, PP2A physical 1 1 1 Affinity Capture-MS
HTA1 YDR225W Histone H2A genetic 1 1 1 Negative Genetic
LEA1 YPL213W Component of U2 snRNP complex genetic 1 1 1 Positive Genetic
DAM1 YGR113W Essential subunit of the Dam1 complex (aka DASH complex) genetic 1 1 1 Negative Genetic
NAB3 YPL190C RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p) genetic 1 1 1 Negative Genetic
MED8 YBR193C Subunit of the RNA polymerase II mediator complex physical 1 1 1 Affinity Capture-MS
DEP1 YAL013W Component of the Rpd3L histone deacetylase complex genetic 1 1 1 Negative Genetic
MNN10 YDR245W Subunit of a Golgi mannosyltransferase complex genetic 1 1 1 Negative Genetic
HOS2 YGL194C Histone deacetylase and subunit of Set3 and Rpd3L complexes genetic 1 1 1 Positive Genetic
CCT6 YDR188W Subunit of the cytosolic chaperonin Cct ring complex genetic 1 1 1 Positive Genetic
MGA2 YIR033W ER membrane protein involved in regulation of OLE1 transcription genetic 1 1 1 Negative Genetic
NEW1 YPL226W Translation termination and ribosome biogenesis factor genetic 1 1 1 Negative Genetic
LTE1 YAL024C Protein similar to GDP/GTP exchange factors genetic 1 1 1 Negative Genetic
FAR11 YNL127W Protein involved in recovery from cell cycle arrest genetic 1 1 1 Negative Genetic
HTB1 YDR224C Histone H2B physical 1 1 1 Affinity Capture-MS
GDE1 YPL110C Glycerophosphocholine (GroPCho) phosphodiesterase physical 1 1 1 Affinity Capture-MS
CDC53 YDL132W Cullin genetic 1 1 1 Negative Genetic
GSF2 YML048W Endoplasmic reticulum (ER) localized integral membrane protein genetic 1 1 1 Negative Genetic
CDC20 YGL116W Activator of anaphase-promoting complex/cyclosome (APC/C) genetic 1 1 1 Negative Genetic
DSK2 YMR276W Nuclear-enriched ubiquitin-like polyubiquitin-binding protein physical 1 1 1 Affinity Capture-Western
HUL5 YGL141W Multiubiquitin chain assembly factor (E4) physical 1 1 1 Affinity Capture-MS
CDC4 YFL009W F-box protein required for both the G1/S and G2/M phase transitions genetic 1 1 1 Negative Genetic
CIN8 YEL061C Bipolar kinesin motor protein physical 1 1 1 Affinity Capture-MS
PDX1 YGR193C E3-binding protein of the mitochondrial pyruvate dehydrogenase complex physical 1 1 1 Affinity Capture-MS
ISU1 YPL135W Conserved protein of the mitochondrial matrix genetic 1 1 1 Negative Genetic
CTF4 YPR135W Chromatin-associated protein genetic 1 1 1 Negative Genetic
BEM4 YPL161C Protein involved in establishment of cell polarity and bud emergence genetic 1 1 1 Positive Genetic
PGM1 YKL127W Phosphoglucomutase, minor isoform genetic 1 1 1 Negative Genetic
PAC2 YER007W Microtubule effector required for tubulin heterodimer formation physical 1 1 1 Affinity Capture-MS
CLB2 YPR119W B-type cyclin involved in cell cycle progression genetic 1 1 1 Dosage Lethality
FAL1 YDR021W Nucleolar protein required for maturation of 18S rRNA genetic 1 1 1 Negative Genetic
FUB1 YCR076C Proteasome-binding protein physical 1 1 1 Affinity Capture-Western
FUB1 YCR076C Proteasome-binding protein genetic 1 1 1 Negative Genetic
BTN2 YGR142W v-SNARE binding protein genetic 1 1 1 Synthetic Rescue
MMS22 YLR320W Subunit of E3 ubiquitin ligase complex involved in replication repair genetic 1 1 1 Negative Genetic
FMN1 YDR236C Riboflavin kinase, produces riboflavin monophosphate (FMN) genetic 1 1 1 Negative Genetic
GTR1 YML121W Subunit of a TORC1-stimulating GTPase and the EGO/GSE complex genetic 1 1 1 Negative Genetic
MMR1 YLR190W Phosphorylated protein of the mitochondrial outer membrane genetic 1 1 1 Negative Genetic
PPG1 YNR032W Serine protein phosphatase involved in formation of FAR complex genetic 1 1 1 Negative Genetic
CDC34 YDR054C Ubiquitin-conjugating enzyme (E2) genetic 1 1 1 Negative Genetic
KTI12 YKL110C Protein that plays a role in modification of tRNA wobble nucleosides genetic 1 1 1 Negative Genetic
APC5 YOR249C Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) genetic 1 1 1 Negative Genetic
APC11 YDL008W Catalytic core subunit, Anaphase-Promoting Complex/Cyclosome (APC/C) genetic 1 1 1 Negative Genetic
CDC27 YBL084C Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C) genetic 1 1 1 Negative Genetic
PEX12 YMR026C C3HC4-type RING-finger peroxin and E3 ubiquitin ligase genetic 1 1 1 Negative Genetic
CHL1 YPL008W Probable DNA helicase genetic 1 1 1 Negative Genetic
BRE5 YNR051C Ubiquitin protease cofactor physical 1 1 1 Affinity Capture-MS
DAL81 YIR023W Positive regulator of genes in multiple nitrogen degradation pathways genetic 1 1 1 Negative Genetic
BFR1 YOR198C Component of mRNP complexes associated with polyribosomes physical 1 1 1 Affinity Capture-RNA
BRN1 YBL097W Subunit of the condensin complex genetic 1 1 1 Negative Genetic
CLB5 YPR120C B-type cyclin involved in DNA replication during S phase genetic 1 1 1 Dosage Lethality
CTF8 YHR191C Subunit of a complex with Ctf18p genetic 1 1 1 Negative Genetic
KAR3 YPR141C Minus-end-directed microtubule motor genetic 1 1 1 Negative Genetic
MAD1 YGL086W Coiled-coil protein involved in spindle-assembly checkpoint genetic 1 1 1 Synthetic Lethality
PHO80 YOL001W Cyclin genetic 1 1 1 Negative Genetic
PAM18 YLR008C Subunit of the import motor (PAM complex) genetic 1 1 1 Negative Genetic
HAP4 YKL109W Transcription factor genetic 1 1 1 Negative Genetic
MOB1 YIL106W Component of the mitotic exit network genetic 1 1 1 Negative Genetic
MPT5 YGL178W mRNA-binding protein of the PUF family physical 1 1 1 Affinity Capture-RNA
MCM7 YBR202W Component of the Mcm2-7 hexameric helicase complex genetic 1 1 1 Negative Genetic
PAM16 YJL104W Subunit of the import motor (PAM complex) genetic 1 1 1 Negative Genetic
BCS1 YDR375C Protein translocase and chaperone required for Complex III assembly genetic 1 1 1 Negative Genetic
EAF7 YNL136W Subunit of the NuA4 histone acetyltransferase complex genetic 1 1 1 Positive Genetic
EFT1 YOR133W Elongation factor 2 (EF-2), also encoded by EFT2 physical 1 1 1 Affinity Capture-Western
AIM44 YPL158C Regulator of Cdc42p and Rho1p genetic 1 1 1 Negative Genetic
PDS5 YMR076C Cohesion maintenance factor physical 1 1 1 Affinity Capture-MS
ILV6 YCL009C Regulatory subunit of acetolactate synthase physical 1 1 1 Affinity Capture-MS
COQ9 YLR201C Protein required for ubiquinone biosynthesis and respiratory growth physical 1 1 1 Affinity Capture-MS
BTS1 YPL069C Geranylgeranyl diphosphate synthase (GGPPS) genetic 1 1 1 Positive Genetic
CSF1 YLR087C Protein with structural similarity to lipid transport protein Vps13p genetic 1 1 1 Negative Genetic
GPI2 YPL076W Protein involved in the synthesis of GlcNAc-PI genetic 1 1 1 Negative Genetic
HRD3 YLR207W ER membrane protein that plays a central role in ERAD genetic 1 1 1 Phenotypic Suppression
COQ10 YOL008W Coenzyme Q (ubiquinone) binding protein genetic 1 1 1 Negative Genetic
MSS4 YDR208W Phosphatidylinositol-4-phosphate 5-kinase genetic 1 1 1 Negative Genetic
PDC2 YDR081C Transcription factor for thiamine-regulated genes genetic 1 1 1 Negative Genetic
COQ11 YLR290C Putative oxidoreductase, subunit of Coenzyme Q biosynthetic complexes physical 1 1 1 Affinity Capture-MS
COQ11 YLR290C Putative oxidoreductase, subunit of Coenzyme Q biosynthetic complexes genetic 1 1 1 Positive Genetic
BRL1 YHR036W Essential nuclear envelope/ER integral membrane protein genetic 1 1 1 Negative Genetic
PMR1 YGL167C High affinity Ca2+/Mn2+ P-type ATPase genetic 1 1 1 Negative Genetic
MSS1 YMR023C Mitochondrial protein genetic 1 1 1 Negative Genetic
COA4 YLR218C Twin Cx(9)C protein involved in cytochrome c oxidase organization genetic 1 1 1 Negative Genetic
POC4 YPL144W Component of a heterodimeric Poc4p-Irc25p chaperone physical 1 1 1 Affinity Capture-MS
POC4 YPL144W Component of a heterodimeric Poc4p-Irc25p chaperone genetic 1 1 1 Negative Genetic
CUZ1 YNL155W Protein with a role in the ubiquitin-proteasome pathway physical 1 1 1 Affinity Capture-Western
IRC25 YLR021W Component of a heterodimeric Poc4p-Irc25p chaperone physical 1 1 1 Affinity Capture-MS
IRC25 YLR021W Component of a heterodimeric Poc4p-Irc25p chaperone genetic 1 1 1 Negative Genetic
MSS18 YPR134W Nuclear encoded protein needed for splicing of mitochondrial intron genetic 1 1 1 Negative Genetic
APJ1 YNL077W Hsp40 chaperone with a role in SUMO-mediated protein degradation physical 1 1 1 Affinity Capture-MS
APJ1 YNL077W Hsp40 chaperone with a role in SUMO-mediated protein degradation genetic 1 1 1 Synthetic Growth Defect
HUR1 YGL168W Protein with a role in DNA repair genetic 1 1 1 Negative Genetic
EOS1 YNL080C Protein involved in N-glycosylation genetic 1 1 1 Negative Genetic
COX10 YPL172C Heme A:farnesyltransferase genetic 1 1 1 Negative Genetic
CYT1 YOR065W Cytochrome c1 genetic 1 1 1 Negative Genetic
MPS3 YJL019W Nuclear envelope protein physical 1 1 1 Affinity Capture-MS
LGE1 YPL055C Protein involved in histone H2B ubiquitination genetic 1 1 1 Negative Genetic
COX23 YHR116W Protein that functions in mitochondrial copper homeostasis genetic 1 1 1 Negative Genetic
CCW12 YLR110C Cell wall mannoprotein genetic 1 1 1 Negative Genetic
CUR1 YPR158W Sorting factor, central regulator of spatial protein quality control genetic 1 1 1 Synthetic Rescue
COX12 YLR038C Subunit VIb of cytochrome c oxidase genetic 1 1 1 Negative Genetic
NCS6 YGL211W Protein required for uridine thiolation of Gln, Lys, and Glu tRNAs genetic 1 1 1 Negative Genetic
CBP4 YGR174C Mitochondrial protein required for assembly of cytochrome bc1 complex genetic 1 1 1 Negative Genetic
MRM2 YGL136C Mitochondrial 2' O-ribose methyltransferase genetic 1 1 1 Negative Genetic
DFR1 YOR236W Dihydrofolate reductase involved in tetrahydrofolate biosynthesis genetic 1 1 1 Positive Genetic
MFM1 YPL060W Mitochondrial inner membrane magnesium transporter genetic 1 1 1 Negative Genetic
ENV9 YOR246C Conserved oxidoreductase involved in lipid droplet morphology genetic 1 1 1 Negative Genetic
ICE2 YIL090W Integral ER membrane protein with type-III transmembrane domains genetic 1 1 1 Negative Genetic
MNE1 YOR350C Protein involved in splicing Group I aI5-beta intron from COX1 mRNA genetic 1 1 1 Negative Genetic