Standard name Systematic name Name description Description
PUF6 YDR496C Pumilio-homology domain protein Pumilio-homology domain protein; binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; co-sediments with the 60S ribosomal subunit and is required for its biogenesis
Visualize the interactome of PUF6
Links to external databases:
  SGD   |   KEGG   |  

Cell cycle phase and timing of transcription peak

Cell cycle phase Time (min)

CYCLoPs localization & abundance

WT1 WT2 WT3
Mito 0.127 0.115 NaN
Nucleolus 0.613 0.608 0.807
Nucleus 0.459 0.419 0.227

GFP localization & abundance

GFP localization GFP abundance
nucleolus, nucleus 21500

GO term count

Metabolism Cell cycle Cell division Signal transduction DNA replication
PUF6 1 0 0 0 0

List of interactors

Use the search utility to find the interactor you are looking for. By clicking the column headers the table will be sorted on that column. Use shift+click to sort on multiple columns. Default sorting is on number of experiments, number of unique methods and the number of publications, and alphabetical on standard name, in that order.
Standard name Systematic name Name description Type #Experiments #Methods #Publications Evidence
LOC1 YFR001W Nucleolar protein involved in the asymmetric localization of ASH1 mRNA physical 7 3 2 Co-localization, Co-localization, Reconstituted Complex, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex
ASH1 YKL185W Component of the Rpd3L histone deacetylase complex physical 6 2 5 Affinity Capture-RNA, Affinity Capture-RNA, Affinity Capture-RNA, Protein-RNA, Protein-RNA, Affinity Capture-RNA
TIF6 YPR016C Constituent of 66S pre-ribosomal particles physical 5 2 4 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western
FUN12 YAL035W Translation initiation factor eIF5B physical 5 3 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex
SHE2 YKL130C RNA-binding protein that binds specific mRNAs and interacts with She3p physical 5 3 3 Affinity Capture-Western, Affinity Capture-Western, Co-localization, Reconstituted Complex, Affinity Capture-Western
LOC1 YFR001W Nucleolar protein involved in the asymmetric localization of ASH1 mRNA genetic 4 4 2 Phenotypic Enhancement, Synthetic Rescue, Synthetic Growth Defect, Phenotypic Suppression
PUF6 YDR496C Pumilio-homology domain protein physical 3 2 3 Affinity Capture-MS, Co-localization, Affinity Capture-MS
SSF1 YHR066W Constituent of 66S pre-ribosomal particles physical 3 2 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-Western
DHH1 YDL160C Cytoplasmic DEAD-box helicase and mRNA decapping activator physical 3 2 3 Affinity Capture-Western, Affinity Capture-RNA, Affinity Capture-RNA
CBF5 YLR175W Pseudouridine synthase catalytic subunit of box H/ACA snoRNPs physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
NOG1 YPL093W Putative GTPase physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
MAK5 YBR142W Essential nucleolar protein physical 3 1 3 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
MEK1 YOR351C Meiosis-specific serine/threonine protein kinase genetic 3 1 3 Negative Genetic, Negative Genetic, Negative Genetic
NOP4 YPL043W Nucleolar protein physical 3 1 2 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
CIC1 YHR052W Essential protein that interacts with proteasome components physical 3 1 2 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
RRP6 YOR001W Nuclear exosome exonuclease component genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
NOP13 YNL175C Nucleolar protein found in preribosomal complexes physical 3 1 2 Affinity Capture-MS, Affinity Capture-MS, Affinity Capture-MS
ERG3 YLR056W C-5 sterol desaturase genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
BRX1 YOL077C Nucleolar protein physical 2 2 2 Affinity Capture-MS, Affinity Capture-Western
NUG1 YER006W GTPase that associates with nuclear 60S pre-ribosomes physical 2 2 2 Affinity Capture-MS, Affinity Capture-Western
NOP56 YLR197W Essential evolutionarily-conserved nucleolar protein genetic 2 2 2 Positive Genetic, Negative Genetic
SBP1 YHL034C Protein that binds eIF4G and has a role in repression of translation physical 2 2 2 Affinity Capture-MS, Affinity Capture-RNA
RPF1 YHR088W Protein involved in assembly and export of the large ribosomal subunit physical 2 2 2 Affinity Capture-MS, Co-purification
ELP3 YPL086C Subunit of Elongator complex genetic 2 2 2 Phenotypic Enhancement, Negative Genetic
NSL1 YPL233W Essential component of the MIND kinetochore complex genetic 2 1 2 Negative Genetic, Negative Genetic
HAS1 YMR290C ATP-dependent RNA helicase physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
NIP7 YPL211W Nucleolar protein required for 60S ribosome subunit biogenesis genetic 2 1 2 Negative Genetic, Negative Genetic
NOP2 YNL061W rRNA m5C methyltransferase physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
NOP2 YNL061W rRNA m5C methyltransferase genetic 2 1 2 Negative Genetic, Negative Genetic
NSA1 YGL111W Constituent of 66S pre-ribosomal particles physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
NUG1 YER006W GTPase that associates with nuclear 60S pre-ribosomes genetic 2 1 2 Negative Genetic, Negative Genetic
LRP1 YHR081W Nuclear exosome-associated nucleic acid binding protein genetic 2 1 2 Negative Genetic, Negative Genetic
RPF2 YKR081C Essential protein involved in rRNA maturation and ribosomal assembly physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
RPL25 YOL127W Ribosomal 60S subunit protein L25 physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
RLP7 YNL002C Nucleolar protein similar to large ribosomal subunit L7 proteins physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
SPB4 YFL002C Putative ATP-dependent RNA helicase physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
RML2 YEL050C Mitochondrial ribosomal protein of the large subunit (L2) genetic 2 1 2 Negative Genetic, Negative Genetic
YPT31 YER031C Rab family GTPase genetic 2 1 2 Negative Genetic, Negative Genetic
RPN11 YFR004W Metalloprotease subunit of 19S regulatory particle genetic 2 1 2 Negative Genetic, Negative Genetic
YBP2 YGL060W Central kinetochore associated protein genetic 2 1 2 Negative Genetic, Negative Genetic
YSR3 YKR053C Dihydrosphingosine 1-phosphate phosphatase genetic 2 1 2 Negative Genetic, Negative Genetic
TRF5 YNL299W Non-canonical poly(A) polymerase genetic 2 1 2 Negative Genetic, Negative Genetic
GCN1 YGL195W Positive regulator of the Gcn2p kinase activity genetic 2 1 2 Positive Genetic, Positive Genetic
NOC4 YPR144C Nucleolar protein physical 2 1 2 Affinity Capture-MS, Affinity Capture-MS
FAR7 YFR008W Protein involved in recovery from pheromone-induced cell cycle arrest genetic 2 1 2 Negative Genetic, Negative Genetic
MPT5 YGL178W mRNA-binding protein of the PUF family physical 2 1 2 Affinity Capture-RNA, Affinity Capture-RNA
PLB3 YOL011W Phospholipase B (lysophospholipase) involved in lipid metabolism genetic 2 1 2 Negative Genetic, Negative Genetic
ILM1 YJR118C Protein of unknown function genetic 2 1 2 Negative Genetic, Negative Genetic
DAK1 YML070W Dihydroxyacetone kinase genetic 2 1 2 Negative Genetic, Negative Genetic
RPL43B YJR094W-A Ribosomal 60S subunit protein L43B physical 2 2 1 Reconstituted Complex, Affinity Capture-Western
RPL43B YJR094W-A Ribosomal 60S subunit protein L43B genetic 2 2 1 Dosage Rescue, Phenotypic Suppression
PAC1 YOR269W Regulator of dynein, involved in intracellular transport genetic 2 2 1 Positive Genetic, Negative Genetic
DST1 YGL043W General transcription elongation factor TFIIS genetic 2 1 1 Positive Genetic, Positive Genetic
FPR4 YLR449W Peptidyl-prolyl cis-trans isomerase (PPIase) physical 2 1 1 Affinity Capture-MS, Affinity Capture-MS
PPQ1 YPL179W Protein phosphatase that regulates the mating response genetic 2 1 1 Negative Genetic, Negative Genetic
ELP6 YMR312W Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex genetic 2 1 1 Negative Genetic, Negative Genetic
SFP1 YLR403W Regulates transcription of ribosomal protein and biogenesis genes regulation 1 1 1 DNA to cDNA expression microarray evidence
YAP6 YDR259C Basic leucine zipper (bZIP) transcription factor regulation 1 1 1 chromatin immunoprecipitation-chip evidence
CKA1 YIL035C Alpha catalytic subunit of casein kinase 2 (CK2) physical 1 1 1 Affinity Capture-MS
CKA2 YOR061W Alpha' catalytic subunit of casein kinase 2 (CK2) physical 1 1 1 Biochemical Activity
ESF1 YDR365C Nucleolar protein involved in pre-rRNA processing physical 1 1 1 Affinity Capture-MS
DBP9 YLR276C DEAD-box protein required for 27S rRNA processing physical 1 1 1 Affinity Capture-MS
DRS1 YLL008W Nucleolar DEAD-box protein required for ribosome assembly and function physical 1 1 1 Affinity Capture-MS
ERB1 YMR049C Constituent of 66S pre-ribosomal particles physical 1 1 1 Affinity Capture-MS
NIP7 YPL211W Nucleolar protein required for 60S ribosome subunit biogenesis physical 1 1 1 Affinity Capture-MS
NOG1 YPL093W Putative GTPase genetic 1 1 1 Negative Genetic
BRR2 YER172C RNA-dependent ATPase RNA helicase (DEIH box) genetic 1 1 1 Negative Genetic
NOC2 YOR206W Protein involved in ribosome biogenesis physical 1 1 1 Affinity Capture-MS
MCM21 YDR318W Component of the kinetochore sub-complex COMA genetic 1 1 1 Negative Genetic
POL5 YEL055C Protein involved in 60S assembly and pre-40S AFs recycling physical 1 1 1 Affinity Capture-MS
KES1 YPL145C Sterol/phosphatidylinositol-4-phosphate (PI(4)P) exchanger genetic 1 1 1 Synthetic Lethality
ARC1 YGL105W Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases genetic 1 1 1 Positive Genetic
NOP58 YOR310C Protein involved in producing mature rRNAs and snoRNAs physical 1 1 1 Affinity Capture-MS
GAR1 YHR089C Protein component of the H/ACA snoRNP pseudouridylase complex physical 1 1 1 Affinity Capture-MS
MTR4 YJL050W RNA duplex-sensing translocase genetic 1 1 1 Negative Genetic
LSM1 YJL124C Lsm (Like Sm) protein genetic 1 1 1 Positive Genetic
NOP1 YDL014W Histone glutamine methyltransferase, modifies H2A at Q105 in nucleolus physical 1 1 1 Affinity Capture-MS
NPL3 YDR432W RNA-binding protein physical 1 1 1 Affinity Capture-MS
NPL3 YDR432W RNA-binding protein genetic 1 1 1 Synthetic Growth Defect
CSL4 YNL232W Exosome non-catalytic core component genetic 1 1 1 Negative Genetic
DIS3 YOL021C Exosome core complex catalytic subunit genetic 1 1 1 Negative Genetic
GCD11 YER025W Gamma subunit of the translation initiation factor eIF2 genetic 1 1 1 Positive Genetic
NUT1 YGL151W Component of the RNA polymerase II mediator complex genetic 1 1 1 Negative Genetic
ARX1 YDR101C Nuclear export factor for the ribosomal pre-60S subunit physical 1 1 1 Affinity Capture-Western
CCR4 YAL021C Component of the CCR4-NOT transcriptional complex physical 1 1 1 Affinity Capture-RNA
CCR4 YAL021C Component of the CCR4-NOT transcriptional complex genetic 1 1 1 Synthetic Rescue
ECM16 YMR128W Essential DEAH-box ATP-dependent RNA helicase specific to U3 snoRNP physical 1 1 1 Affinity Capture-MS
LSG1 YGL099W Putative GTPase involved in 60S ribosomal subunit biogenesis genetic 1 1 1 Negative Genetic
NAB2 YGL122C Nuclear polyadenylated RNA-binding protein physical 1 1 1 Affinity Capture-RNA
TAF1 YGR274C TFIID subunit, involved in RNA pol II transcription initiation physical 1 1 1 Affinity Capture-MS
SPT2 YER161C Protein involved in negative regulation of transcription physical 1 1 1 Affinity Capture-MS
RLP24 YLR009W Essential protein required for ribosomal large subunit biogenesis physical 1 1 1 Affinity Capture-MS
SPT5 YML010W Spt4p/5p (DSIF) transcription elongation factor complex subunit physical 1 1 1 Affinity Capture-MS
STM1 YLR150W Protein required for optimal translation under nutrient stress physical 1 1 1 Affinity Capture-MS
RIX7 YLL034C Putative ATPase of the AAA family physical 1 1 1 Affinity Capture-MS
RPL20A YMR242C Ribosomal 60S subunit protein L20A physical 1 1 1 Affinity Capture-MS
RPL3 YOR063W Ribosomal 60S subunit protein L3 physical 1 1 1 Affinity Capture-MS
RPL7B YPL198W Ribosomal 60S subunit protein L7B physical 1 1 1 Affinity Capture-MS
RPL27A YHR010W Ribosomal 60S subunit protein L27A physical 1 1 1 Affinity Capture-MS
RLP7 YNL002C Nucleolar protein similar to large ribosomal subunit L7 proteins genetic 1 1 1 Negative Genetic
RPF1 YHR088W Protein involved in assembly and export of the large ribosomal subunit genetic 1 1 1 Negative Genetic
RPL15B YMR121C Ribosomal 60S subunit protein L15B physical 1 1 1 Affinity Capture-MS
RPL23A YBL087C Ribosomal 60S subunit protein L23A physical 1 1 1 Affinity Capture-MS
RPL8B YLL045C Ribosomal 60S subunit protein L8A physical 1 1 1 Affinity Capture-MS
RPP2B YDR382W Ribosomal protein P2 beta physical 1 1 1 Affinity Capture-MS
YTM1 YOR272W Ribosomal assembly factor and 66S pre-ribosomal particle constituent physical 1 1 1 Affinity Capture-MS
RPL1A YPL220W Ribosomal 60S subunit protein L1A physical 1 1 1 Affinity Capture-MS
RRP12 YPL012W Protein required for export of the ribosomal subunits physical 1 1 1 Affinity Capture-MS
RPL4B YDR012W Ribosomal 60S subunit protein L4B physical 1 1 1 Affinity Capture-MS
RPL5 YPL131W Ribosomal 60S subunit protein L5 physical 1 1 1 Affinity Capture-MS
RRP5 YMR229C RNA binding protein involved in synthesis of 18S and 5.8S rRNAs physical 1 1 1 Affinity Capture-MS
URB1 YKL014C Protein required for the normal accumulation of 25S and 5.8S rRNAs physical 1 1 1 Affinity Capture-MS
RRP15 YPR143W Nucleolar protein physical 1 1 1 Affinity Capture-MS
UBP3 YER151C Ubiquitin-specific protease that cleaves Ub-protein fusions physical 1 1 1 Affinity Capture-MS
RIX1 YHR197W Component of the Rix1 complex and possibly pre-replicative complexes physical 1 1 1 Affinity Capture-MS
TAF5 YBR198C Subunit (90 kDa) of TFIID and SAGA complexes physical 1 1 1 Affinity Capture-MS
ULP2 YIL031W Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins physical 1 1 1 Affinity Capture-MS
VAM6 YDL077C Guanine nucleotide exchange factor for the GTPase Gtr1p physical 1 1 1 Affinity Capture-MS
RPC82 YPR190C RNA polymerase III subunit C82 physical 1 1 1 Affinity Capture-MS
RPL16A YIL133C Ribosomal 60S subunit protein L16A physical 1 1 1 Affinity Capture-MS
RPL8A YHL033C Ribosomal 60S subunit protein L8A physical 1 1 1 Affinity Capture-Western
RPL8A YHL033C Ribosomal 60S subunit protein L8A genetic 1 1 1 Positive Genetic
SUP35 YDR172W Translation termination factor eRF3 physical 1 1 1 Affinity Capture-MS
SUP35 YDR172W Translation termination factor eRF3 genetic 1 1 1 Positive Genetic
UTP10 YJL109C Nucleolar protein physical 1 1 1 Affinity Capture-MS
TFC6 YDR362C Subunit of RNA polymerase III transcription initiation factor complex physical 1 1 1 Affinity Capture-MS
UTP14 YML093W Subunit of U3-containing Small Subunit (SSU) processome complex physical 1 1 1 Affinity Capture-MS
STE12 YHR084W Transcription factor that is activated by a MAPK signaling cascade physical 1 1 1 Affinity Capture-RNA
RPL24A YGL031C Ribosomal 60S subunit protein L24A physical 1 1 1 Affinity Capture-MS
RPL11A YPR102C Ribosomal 60S subunit protein L11A physical 1 1 1 Affinity Capture-MS
RPS0A YGR214W Ribosomal 40S subunit protein S0A physical 1 1 1 Affinity Capture-MS
RPS1A YLR441C Ribosomal protein of the small (40S) subunit physical 1 1 1 Affinity Capture-MS
RPS2 YGL123W Protein component of the small (40S) subunit physical 1 1 1 Affinity Capture-MS
RPS6A YPL090C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS7A YOR096W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS8A YBL072C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS9A YPL081W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS10A YOR293W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS12 YOR369C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS13 YDR064W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS15 YOL040C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS17A YML024W Ribosomal protein 51 (rp51) of the small (40s) subunit physical 1 1 1 Affinity Capture-MS
RPS18A YDR450W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS19A YOL121C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS20 YHL015W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS22A YJL190C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPL26A YLR344W Ribosomal 60S subunit protein L26A physical 1 1 1 Affinity Capture-MS
RPS27A YKL156W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPL31A YDL075W Ribosomal 60S subunit protein L31A physical 1 1 1 Affinity Capture-MS
RPS3 YNL178W Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RPS14A YCR031C Protein component of the small (40S) ribosomal subunit physical 1 1 1 Affinity Capture-MS
RRP6 YOR001W Nuclear exosome exonuclease component physical 1 1 1 Affinity Capture-MS
RRP43 YCR035C Exosome non-catalytic core component physical 1 1 1 Affinity Capture-MS
SEC14 YMR079W Phosphatidylinositol/phosphatidylcholine transfer protein genetic 1 1 1 Synthetic Lethality
XRN1 YGL173C Evolutionarily-conserved 5'-3' exonuclease genetic 1 1 1 Negative Genetic
PUS7 YOR243C Pseudouridine synthase genetic 1 1 1 Negative Genetic
RRP8 YDR083W Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase genetic 1 1 1 Positive Genetic
SEH1 YGL100W Subunit of the Nup84 nuclear pore and SEACAT subcomplexes genetic 1 1 1 Positive Genetic
TGS1 YPL157W Trimethyl guanosine synthase, conserved nucleolar methyl transferase genetic 1 1 1 Positive Genetic
URM1 YIL008W Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs genetic 1 1 1 Synthetic Growth Defect
UBA4 YHR111W E1-like protein that activates Urm1p before urmylation genetic 1 1 1 Synthetic Growth Defect
TAF8 YML114C TFIID subunit (65 kDa) genetic 1 1 1 Negative Genetic
TOM70 YNL121C Component of the TOM (translocase of outer membrane) complex genetic 1 1 1 Negative Genetic
VPS27 YNR006W Endosomal protein that forms a complex with Hse1p genetic 1 1 1 Negative Genetic
SRS2 YJL092W DNA helicase and DNA-dependent ATPase genetic 1 1 1 Dosage Lethality
TEL2 YGR099W Subunit of the ASTRA complex, involved in chromatin remodeling genetic 1 1 1 Positive Genetic
RPN5 YDL147W Subunit of the CSN and 26S proteasome lid complexes genetic 1 1 1 Positive Genetic
YIP1 YGR172C Integral membrane protein genetic 1 1 1 Positive Genetic
YKT6 YKL196C Vesicle membrane protein (v-SNARE) with acyltransferase activity genetic 1 1 1 Positive Genetic
RPL10 YLR075W Ribosomal 60S subunit protein L10 genetic 1 1 1 Positive Genetic
RPB8 YOR224C RNA polymerase subunit ABC14.5 genetic 1 1 1 Positive Genetic
YAL064W YAL064W Protein of unknown function genetic 1 1 1 Positive Genetic
SHE1 YBL031W Mitotic spindle protein genetic 1 1 1 Positive Genetic
SHG1 YBR258C Subunit of the COMPASS (Set1C) complex genetic 1 1 1 Positive Genetic
RPS29B YDL061C Protein component of the small (40S) ribosomal subunit genetic 1 1 1 Positive Genetic
SRO7 YPR032W Effector of Rab GTPase Sec4p genetic 1 1 1 Positive Genetic
RPL40A YIL148W Ubiquitin-ribosomal 60S subunit protein L40A fusion protein genetic 1 1 1 Positive Genetic
YJL047C-A YJL047C-A Putative protein of unknown function genetic 1 1 1 Positive Genetic
UFO1 YML088W F-box receptor protein genetic 1 1 1 Positive Genetic
YPL077C YPL077C Putative protein of unknown function genetic 1 1 1 Positive Genetic
RFT1 YBL020W Membrane protein required for translocation of Man5GlcNac2-PP-Dol genetic 1 1 1 Negative Genetic
RPN6 YDL097C Essential, non-ATPase regulatory subunit of the 26S proteasome lid genetic 1 1 1 Negative Genetic
RRP42 YDL111C Exosome non-catalytic core component genetic 1 1 1 Negative Genetic
SEC31 YDL195W Component of the Sec13p-Sec31p complex of the COPII vesicle coat genetic 1 1 1 Negative Genetic
UTR5 YEL035C Protein of unknown function genetic 1 1 1 Negative Genetic
RSP5 YER125W NEDD4 family E3 ubiquitin ligase genetic 1 1 1 Negative Genetic
SEC27 YGL137W Essential beta'-coat protein of the COPI coatomer genetic 1 1 1 Negative Genetic
RPN1 YHR027C Non-ATPase base subunit of the 19S RP of the 26S proteasome genetic 1 1 1 Negative Genetic
RRP4 YHR069C Exosome non-catalytic core component genetic 1 1 1 Negative Genetic
QNS1 YHR074W Glutamine-dependent NAD(+) synthetase genetic 1 1 1 Negative Genetic
RIA1 YNL163C Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis genetic 1 1 1 Negative Genetic
SEC2 YNL272C Guanyl-nucleotide exchange factor for the small G-protein Sec4p genetic 1 1 1 Negative Genetic
RPT5 YOR117W ATPase of the 19S regulatory particle of the 26S proteasome genetic 1 1 1 Negative Genetic
RPT4 YOR259C ATPase of the 19S regulatory particle of the 26S proteasome genetic 1 1 1 Negative Genetic
TAH18 YPR048W Conserved NAPDH-dependent diflavin reductase genetic 1 1 1 Negative Genetic
RPN7 YPR108W Essential non-ATPase regulatory subunit of the 26S proteasome genetic 1 1 1 Negative Genetic
SPO7 YAL009W Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme genetic 1 1 1 Negative Genetic
SHP1 YBL058W UBX domain-containing substrate adaptor for Cdc48p genetic 1 1 1 Negative Genetic
YBR096W YBR096W Putative protein of unknown function genetic 1 1 1 Negative Genetic
TVS1 YCR061W Protein of unknown function genetic 1 1 1 Negative Genetic
RSM24 YDR175C Mitochondrial ribosomal protein of the small subunit genetic 1 1 1 Negative Genetic
SAP1 YER047C Putative ATPase of the AAA family genetic 1 1 1 Negative Genetic
RIM8 YGL045W Protein involved in proteolytic activation of Rim101p genetic 1 1 1 Negative Genetic
YHL008C YHL008C Putative protein of unknown function genetic 1 1 1 Negative Genetic
RPN10 YHR200W Proteasome polyubiquitin receptor genetic 1 1 1 Negative Genetic
SNX4 YJL036W Sorting nexin genetic 1 1 1 Negative Genetic
VPS35 YJL154C Endosomal subunit of membrane-associated retromer complex genetic 1 1 1 Negative Genetic
TMA22 YJR014W Protein of unknown function genetic 1 1 1 Negative Genetic
REC107 YJR021C Protein involved in early stages of meiotic recombination genetic 1 1 1 Negative Genetic
SIC1 YLR079W Cyclin-dependent kinase inhibitor (CKI) genetic 1 1 1 Negative Genetic
STE23 YLR389C Metalloprotease genetic 1 1 1 Negative Genetic
RIM9 YMR063W Plasma membrane protein of unknown function genetic 1 1 1 Negative Genetic
RPS16A YMR143W Protein component of the small (40S) ribosomal subunit genetic 1 1 1 Negative Genetic
RIM20 YOR275C Protein involved in proteolytic activation of Rim101p genetic 1 1 1 Negative Genetic
YPL191C YPL191C K48-specific deubiquitinating (DUB) enzyme genetic 1 1 1 Negative Genetic
RMD8 YFR048W Cytosolic protein required for sporulation genetic 1 1 1 Negative Genetic
YHL017W YHL017W Putative protein of unknown function genetic 1 1 1 Negative Genetic
SRX1 YKL086W Sulfiredoxin genetic 1 1 1 Negative Genetic
YKL096C-B YKL096C-B Putative protein of unknown function genetic 1 1 1 Negative Genetic
YML018C YML018C Protein of unknown function genetic 1 1 1 Negative Genetic
VAN1 YML115C Component of the mannan polymerase I genetic 1 1 1 Negative Genetic
VAC7 YNL054W Integral vacuolar membrane protein genetic 1 1 1 Negative Genetic
SCH9 YHR205W AGC family protein kinase genetic 1 1 1 Synthetic Growth Defect
RHO2 YNL090W Non-essential small GTPase of the Rho/Rac family of Ras-like proteins genetic 1 1 1 Negative Genetic
YNL194C YNL194C Integral membrane protein genetic 1 1 1 Negative Genetic
SLA2 YNL243W Adaptor protein that links actin to clathrin and endocytosis genetic 1 1 1 Negative Genetic
YOL162W YOL162W Putative protein of unknown function genetic 1 1 1 Negative Genetic
VPS21 YOR089C Endosomal Rab family GTPase genetic 1 1 1 Negative Genetic
YOR186W YOR186W Putative protein of unknown function genetic 1 1 1 Negative Genetic
REC8 YPR007C Meiosis-specific component of the sister chromatid cohesion complex genetic 1 1 1 Negative Genetic
RPL38 YLR325C Ribosomal 60S subunit protein L38 genetic 1 1 1 Negative Genetic
SFM1 YOR021C SPOUT methyltransferase genetic 1 1 1 Negative Genetic
VAM7 YGL212W Vacuolar SNARE protein genetic 1 1 1 Negative Genetic
TUB4 YLR212C Gamma-tubulin genetic 1 1 1 Synthetic Growth Defect
SGS1 YMR190C RecQ family nucleolar DNA helicase genetic 1 1 1 Negative Genetic
ACC1 YNR016C Acetyl-CoA carboxylase, biotin containing enzyme genetic 1 1 1 Negative Genetic
BEM1 YBR200W Protein containing SH3-domains genetic 1 1 1 Positive Genetic
ASC1 YMR116C G-protein beta subunit and guanine dissociation inhibitor for Gpa2p physical 1 1 1 Affinity Capture-MS
BUD20 YLR074C C2H2-type zinc finger protein required for ribosome assembly physical 1 1 1 Affinity Capture-MS
CAR1 YPL111W Arginase, catabolizes arginine to ornithine and urea genetic 1 1 1 Negative Genetic
CDC12 YHR107C Component of the septin ring that is required for cytokinesis genetic 1 1 1 Negative Genetic
DBP10 YDL031W Putative ATP-dependent RNA helicase of the DEAD-box protein family physical 1 1 1 Affinity Capture-MS
IPI3 YNL182C Component of the Rix1 complex and pre-replicative complexes (pre-RCs) genetic 1 1 1 Negative Genetic
NOG2 YNR053C Putative GTPase physical 1 1 1 Affinity Capture-Western
COP1 YDL145C Alpha subunit of COPI vesicle coatomer complex genetic 1 1 1 Negative Genetic
ENP1 YBR247C Protein associated with U3 and U14 snoRNAs physical 1 1 1 Affinity Capture-MS
EXO84 YBR102C Exocyst subunit with dual roles in exocytosis and spliceosome assembly genetic 1 1 1 Negative Genetic
HTB2 YBL002W Histone H2B physical 1 1 1 Affinity Capture-MS
IOC3 YFR013W Subunit of the Isw1a complex genetic 1 1 1 Negative Genetic
MRD1 YPR112C Essential conserved small ribosomal subunit (40s) synthesis factor genetic 1 1 1 Positive Genetic
KRR1 YCL059C Nucleolar protein required for rRNA synthesis and ribosomal assembly physical 1 1 1 Affinity Capture-MS
KRR1 YCL059C Nucleolar protein required for rRNA synthesis and ribosomal assembly genetic 1 1 1 Positive Genetic
MRPL9 YGR220C Mitochondrial ribosomal protein of the large subunit genetic 1 1 1 Negative Genetic
CMR1 YDL156W Nuclear protein with role in transcription, protein quality control genetic 1 1 1 Positive Genetic
MTR10 YOR160W Nuclear import receptor genetic 1 1 1 Negative Genetic
CMD1 YBR109C Calmodulin genetic 1 1 1 Negative Genetic
MYO2 YOR326W Type V myosin motor involved in actin-based transport of cargos genetic 1 1 1 Negative Genetic
ARP8 YOR141C Nuclear actin-related protein involved in chromatin remodeling genetic 1 1 1 Negative Genetic
ITC1 YGL133W Subunit of ATP-dependent Isw2p-Itc1p chromatin remodeling complex genetic 1 1 1 Negative Genetic
NMD3 YHR170W Protein involved in nuclear export of the large ribosomal subunit physical 1 1 1 Co-fractionation
NOT3 YIL038C Component of the CCR4-NOT core complex, involved in mRNA decapping genetic 1 1 1 Positive Genetic
NUP159 YIL115C FG-nucleoporin component of central core of the nuclear pore complex genetic 1 1 1 Positive Genetic
POL1 YNL102W Catalytic subunit of the DNA polymerase I alpha-primase complex genetic 1 1 1 Synthetic Rescue
POP7 YBR167C Subunit of RNase MRP, nuclear RNase P and telomerase genetic 1 1 1 Positive Genetic
HTA1 YDR225W Histone H2A genetic 1 1 1 Negative Genetic
PRP4 YPR178W Splicing factor genetic 1 1 1 Negative Genetic
PRP21 YJL203W Subunit of the SF3a splicing factor complex genetic 1 1 1 Negative Genetic
POL2 YNL262W Catalytic subunit of DNA polymerase (II) epsilon genetic 1 1 1 Synthetic Rescue
HTA2 YBL003C Histone H2A physical 1 1 1 Affinity Capture-MS
ESF2 YNR054C Essential nucleolar protein involved in pre-18S rRNA processing genetic 1 1 1 Negative Genetic
PEP4 YPL154C Vacuolar aspartyl protease (proteinase A) genetic 1 1 1 Negative Genetic
PTI1 YGR156W Essential component of CPF (cleavage and polyadenylation factor) genetic 1 1 1 Positive Genetic
MED8 YBR193C Subunit of the RNA polymerase II mediator complex genetic 1 1 1 Positive Genetic
DEP1 YAL013W Component of the Rpd3L histone deacetylase complex genetic 1 1 1 Positive Genetic
CDC25 YLR310C Membrane bound guanine nucleotide exchange factor genetic 1 1 1 Positive Genetic
BRR1 YPR057W snRNP protein component of spliceosomal snRNPs genetic 1 1 1 Positive Genetic
GCN5 YGR252W Catalytic subunit of ADA and SAGA histone acetyltransferase complexes physical 1 1 1 Affinity Capture-MS
MLP1 YKR095W Myosin-like protein associated with the nuclear envelope genetic 1 1 1 Positive Genetic
CCT6 YDR188W Subunit of the cytosolic chaperonin Cct ring complex genetic 1 1 1 Positive Genetic
BCY1 YIL033C Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA) genetic 1 1 1 Positive Genetic
HSE1 YHL002W Subunit of the endosomal Vps27p-Hse1p complex genetic 1 1 1 Negative Genetic
LTV1 YKL143W Subunit of the EGO/GSE complex genetic 1 1 1 Positive Genetic
PUF3 YLL013C Protein of the mitochondrial outer surface physical 1 1 1 Affinity Capture-RNA
CDC13 YDL220C Single stranded DNA-binding protein found at TG1-3 telomere G-tails genetic 1 1 1 Synthetic Rescue
GPH1 YPR160W Glycogen phosphorylase required for the mobilization of glycogen genetic 1 1 1 Negative Genetic
GAS1 YMR307W Beta-1,3-glucanosyltransferase required for cell wall assembly genetic 1 1 1 Negative Genetic
CAF20 YOR276W Phosphoprotein of the mRNA cap-binding complex physical 1 1 1 Affinity Capture-MS
NMD5 YJR132W Karyopherin genetic 1 1 1 Positive Genetic
BUB2 YMR055C Mitotic exit network regulator genetic 1 1 1 Negative Genetic
HTB1 YDR224C Histone H2B genetic 1 1 1 Negative Genetic
CDC53 YDL132W Cullin genetic 1 1 1 Negative Genetic
GSF2 YML048W Endoplasmic reticulum (ER) localized integral membrane protein genetic 1 1 1 Negative Genetic
POR1 YNL055C Mitochondrial porin (voltage-dependent anion channel) genetic 1 1 1 Negative Genetic
NOP6 YDL213C rRNA-binding protein required for 40S ribosomal subunit biogenesis physical 1 1 1 Affinity Capture-MS
PMA1 YGL008C Plasma membrane P2-type H+-ATPase genetic 1 1 1 Negative Genetic
KOG1 YHR186C Subunit of TORC1 genetic 1 1 1 Negative Genetic
LIA1 YJR070C Deoxyhypusine hydroxylase genetic 1 1 1 Negative Genetic
PBR1 YNL181W Putative oxidoreductase genetic 1 1 1 Negative Genetic
ACS2 YLR153C Acetyl-coA synthetase isoform genetic 1 1 1 Negative Genetic
ERG10 YPL028W Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase) genetic 1 1 1 Negative Genetic
ELA1 YNL230C Elongin A physical 1 1 1 Affinity Capture-MS
COS111 YBR203W Protein required for antifungal drug ciclopirox olamine resistance genetic 1 1 1 Positive Genetic
EST1 YLR233C TLC1 RNA-associated factor involved in telomere length regulation physical 1 1 1 Affinity Capture-MS
EST1 YLR233C TLC1 RNA-associated factor involved in telomere length regulation genetic 1 1 1 Synthetic Growth Defect
MAK11 YKL021C Protein involved in an early step of 60S ribosomal subunit biogenesis physical 1 1 1 Affinity Capture-MS
MAK11 YKL021C Protein involved in an early step of 60S ribosomal subunit biogenesis genetic 1 1 1 Positive Genetic
CDC4 YFL009W F-box protein required for both the G1/S and G2/M phase transitions genetic 1 1 1 Negative Genetic
PPE1 YHR075C Protein with carboxyl methyl esterase activity genetic 1 1 1 Negative Genetic
LAS1 YKR063C Endonuclease involved in pre-rRNA processing at both ends of ITS2 genetic 1 1 1 Negative Genetic
ECM33 YBR078W GPI-anchored protein involved in efficient glucose uptake genetic 1 1 1 Negative Genetic
MDH2 YOL126C Cytoplasmic malate dehydrogenase genetic 1 1 1 Negative Genetic
ELG1 YOR144C Subunit of an alternative replication factor C complex genetic 1 1 1 Positive Genetic
DBF2 YGR092W Ser/Thr kinase involved in transcription and stress response genetic 1 1 1 Negative Genetic
PMI40 YER003C Mannose-6-phosphate isomerase genetic 1 1 1 Negative Genetic
EXO70 YJL085W Subunit of the exocyst complex genetic 1 1 1 Negative Genetic
BIR1 YJR089W Subunit of chromosomal passenger complex (CPC) genetic 1 1 1 Positive Genetic
MMS22 YLR320W Subunit of E3 ubiquitin ligase complex involved in replication repair physical 1 1 1 Affinity Capture-MS
DCI1 YOR180C Peroxisomal protein genetic 1 1 1 Negative Genetic
NDC80 YIL144W Component of the kinetochore-associated Ndc80 complex genetic 1 1 1 Negative Genetic
AIM18 YHR198C Protein of unknown function genetic 1 1 1 Positive Genetic
COG6 YNL041C Component of the conserved oligomeric Golgi complex genetic 1 1 1 Positive Genetic
IKI1 YHR187W Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex genetic 1 1 1 Negative Genetic
IPL1 YPL209C Aurora kinase of chromosomal passenger complex genetic 1 1 1 Positive Genetic
BRE5 YNR051C Ubiquitin protease cofactor physical 1 1 1 Affinity Capture-MS
JIP5 YPR169W Protein required for biogenesis of the large ribosomal subunit physical 1 1 1 Affinity Capture-MS
CBF1 YJR060W Basic helix-loop-helix (bHLH) protein genetic 1 1 1 Negative Genetic
MPS1 YDL028C Dual-specificity kinase genetic 1 1 1 Positive Genetic
MPT5 YGL178W mRNA-binding protein of the PUF family genetic 1 1 1 Synthetic Rescue
ABF1 YKL112W DNA binding protein with possible chromatin-reorganizing activity genetic 1 1 1 Negative Genetic
BCH1 YMR237W Member of the ChAPs family (Chs5p-Arf1p-binding proteins) genetic 1 1 1 Negative Genetic
CUS2 YNL286W Putative checkpoint factor in transcription genetic 1 1 1 Negative Genetic
PRP16 YKR086W DEAH-box RNA helicase genetic 1 1 1 Negative Genetic
DJP1 YIR004W ER-associated chaperone involved in protein targeting genetic 1 1 1 Negative Genetic
ORC5 YNL261W Subunit of the origin recognition complex (ORC) genetic 1 1 1 Negative Genetic
AZF1 YOR113W Zinc-finger transcription factor genetic 1 1 1 Negative Genetic
CLA4 YNL298W Cdc42p-activated signal transducing kinase genetic 1 1 1 Negative Genetic
NIP100 YPL174C Large subunit of the dynactin complex genetic 1 1 1 Negative Genetic
ALG5 YPL227C UDP-glucose:dolichyl-phosphate glucosyltransferase genetic 1 1 1 Negative Genetic
NUP188 YML103C Subunit of the inner ring of the nuclear pore complex (NPC) genetic 1 1 1 Positive Genetic
KAP120 YPL125W Karyopherin responsible for the nuclear import of Rpf1p genetic 1 1 1 Positive Genetic
ATM1 YMR301C Mitochondrial inner membrane ATP-binding cassette (ABC) transporter genetic 1 1 1 Negative Genetic
MOG1 YJR074W Conserved nuclear protein that interacts with GTP-Gsp1p genetic 1 1 1 Positive Genetic
ECM21 YBL101C Alpha-arrestin, ubiquitin ligase adaptor for Rsp5p genetic 1 1 1 Negative Genetic
ALG1 YBR110W Mannosyltransferase genetic 1 1 1 Negative Genetic
BTT1 YDR252W Heterotrimeric nascent polypeptide-associated complex beta3 subunit genetic 1 1 1 Negative Genetic
HRQ1 YDR291W 3'-5' DNA helicase of the conserved RecQ family genetic 1 1 1 Negative Genetic
MSS4 YDR208W Phosphatidylinositol-4-phosphate 5-kinase genetic 1 1 1 Negative Genetic
BUL2 YML111W Alpha-arrestin, component of the Rsp5p E3-ubiquitin ligase complex physical 1 1 1 Affinity Capture-MS
PUF4 YGL014W Member of the PUF protein family physical 1 1 1 Affinity Capture-RNA
ADE16 YLR028C Enzyme of 'de novo' purine biosynthesis genetic 1 1 1 Positive Genetic
BRL1 YHR036W Essential nuclear envelope/ER integral membrane protein genetic 1 1 1 Positive Genetic
BYE1 YKL005C Negative regulator of transcription elongation genetic 1 1 1 Negative Genetic
GIS2 YNL255C Translational activator for mRNAs with internal ribosome entry sites physical 1 1 1 Affinity Capture-MS
OGG1 YML060W Nuclear and mitochondrial glycosylase/lyase genetic 1 1 1 Negative Genetic
MRPL27 YBR282W Mitochondrial ribosomal protein of the large subunit genetic 1 1 1 Negative Genetic
CSA1 YPR174C Protein of unknown function genetic 1 1 1 Negative Genetic
APJ1 YNL077W Hsp40 chaperone with a role in SUMO-mediated protein degradation physical 1 1 1 Affinity Capture-MS
GUP1 YGL084C Plasma membrane protein involved in remodeling GPI anchors genetic 1 1 1 Negative Genetic
NEJ1 YLR265C DNA end-binding protein required for nonhomologous end joining (NHEJ) genetic 1 1 1 Positive Genetic
ERG24 YNL280C C-14 sterol reductase genetic 1 1 1 Negative Genetic
NCR1 YPL006W Vacuolar membrane protein involved with Npc2p in sterol trafficking genetic 1 1 1 Negative Genetic
CDC50 YCR094W Endosomal protein that interacts with phospholipid flippase Drs2p genetic 1 1 1 Negative Genetic
HVG1 YER039C Protein of unknown function genetic 1 1 1 Negative Genetic
LGE1 YPL055C Protein involved in histone H2B ubiquitination genetic 1 1 1 Negative Genetic
GPI13 YLL031C ER membrane localized phosphoryltransferase genetic 1 1 1 Positive Genetic
MET5 YJR137C Sulfite reductase beta subunit genetic 1 1 1 Negative Genetic
NCS6 YGL211W Protein required for uridine thiolation of Gln, Lys, and Glu tRNAs genetic 1 1 1 Negative Genetic
HTS1 YPR033C Cytoplasmic and mitochondrial histidine tRNA synthetase genetic 1 1 1 Negative Genetic
ARR1 YPR199C Transcriptional activator of the basic leucine zipper (bZIP) family genetic 1 1 1 Negative Genetic
MOD5 YOR274W Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase genetic 1 1 1 Negative Genetic
ERG8 YMR220W Phosphomevalonate kinase genetic 1 1 1 Negative Genetic
HMX1 YLR205C ER localized heme oxygenase genetic 1 1 1 Negative Genetic
AVT7 YIL088C Vacuolar amino acid transporter genetic 1 1 1 Negative Genetic
ATX2 YOR079C Golgi membrane protein involved in manganese homeostasis genetic 1 1 1 Negative Genetic
PET8 YNL003C S-adenosylmethionine transporter of the mitochondrial inner membrane genetic 1 1 1 Positive Genetic
PFF1 YBR074W Multi-spanning vacuolar membrane protease genetic 1 1 1 Negative Genetic
PET100 YDR079W Chaperone that facilitates the assembly of cytochrome c oxidase genetic 1 1 1 Positive Genetic
FLO10 YKR102W Member of the FLO family of cell wall flocculation proteins genetic 1 1 1 Negative Genetic
KCH1 YJR054W Potassium transporter that mediates K+ influx genetic 1 1 1 Negative Genetic
ADF1 YCL058W-A Transcriptional repressor encoded by the FYV5 antisense strand genetic 1 1 1 Positive Genetic
PAU23 YLR037C Cell wall mannoprotein genetic 1 1 1 Negative Genetic