Standard name Systematic name Name description Description
VPH1 YOR270C Subunit a of the vacuolar-ATPase V0 domain Subunit a of the vacuolar-ATPase V0 domain; encodes one of two isoforms, located in vacuolar V-ATPase complexes while STV1 encodes the second isoform and is located in Golgi and endosomal V-ATPase complexes; interaction with PI(3,5)P2 increases V-ATPase activity and contributes to hyperosmotic stress tolerance; relative distribution to the vacuolar membrane decreases upon DNA replication stress; human homolog ATP6V0A4 implicated in renal tubular acidosis, can complement yeast null mutant
Visualize the interactome of VPH1
Links to external databases:
  SGD   |   KEGG   |  

Cell cycle phase and timing of transcription peak

Cell cycle phase Time (min)

CYCLoPs localization & abundance

WT1 WT2 WT3
Cytoplasm 0.059 NaN NaN
Mito 0.068 0.098 0.103
Nucleolus 0.112 0.122 0.101
Nucleus 0.068 0.078 NaN
Vac/Vac Memb 0.832 0.824 0.907

GFP localization & abundance

GFP localization GFP abundance
vacuolar membrane 55700

GO term count

Metabolism Cell cycle Cell division Signal transduction DNA replication
VPH1 1 0 0 0 0

Enzyme information

Catalyzed reactions in Yeast8
r_1086 (V-ATPase, vacuole)

List of interactors

Use the search utility to find the interactor you are looking for. By clicking the column headers the table will be sorted on that column. Use shift+click to sort on multiple columns. Default sorting is on number of experiments, number of unique methods and the number of publications, and alphabetical on standard name, in that order.
Standard name Systematic name Name description Type #Experiments #Methods #Publications Evidence
STV1 YMR054W Subunit a of the vacuolar-ATPase V0 domain genetic 13 6 10 Dosage Rescue, Negative Genetic, Negative Genetic, Synthetic Growth Defect, Phenotypic Enhancement, Phenotypic Enhancement, Synthetic Lethality, Dosage Rescue, Negative Genetic, Synthetic Growth Defect, Synthetic Rescue, Negative Genetic, Negative Genetic
VMA1 YDL185W Subunit A of the V1 peripheral membrane domain of V-ATPase physical 13 5 10 Affinity Capture-Western, Two-hybrid, Two-hybrid, Affinity Capture-Western, Affinity Capture-Western, Co-fractionation, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, Co-purification
VMA6 YLR447C Subunit d of the V0 integral membrane domain of V-ATPase physical 12 6 11 Affinity Capture-Western, Affinity Capture-Western, Co-fractionation, Affinity Capture-MS, Affinity Capture-MS, PCA, Affinity Capture-Western, Affinity Capture-Western, Reconstituted Complex, Reconstituted Complex, Affinity Capture-Western, Co-purification
VMA13 YPR036W Subunit H of the V1 peripheral membrane domain of V-ATPase physical 10 7 9 Reconstituted Complex, Affinity Capture-Western, Co-fractionation, PCA, Affinity Capture-Western, Two-hybrid, Reconstituted Complex, PCA, Protein-peptide, Co-crystal Structure
VMA2 YBR127C Subunit B of V1 peripheral membrane domain of vacuolar H+-ATPase physical 7 4 7 Affinity Capture-Western, Co-fractionation, Affinity Capture-MS, Affinity Capture-Western, Affinity Capture-Western, Affinity Capture-Western, FRET
VMA7 YGR020C Subunit F of the V1 peripheral membrane domain of V-ATPase physical 7 4 6 Affinity Capture-MS, Affinity Capture-Western, Co-fractionation, Affinity Capture-MS, Affinity Capture-MS, PCA, Affinity Capture-Western
VMA5 YKL080W Subunit C of the V1 peripheral membrane domain of V-ATPase physical 6 5 6 Affinity Capture-Western, Co-fractionation, Affinity Capture-Western, Reconstituted Complex, Protein-peptide, FRET
VMA4 YOR332W Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase) physical 5 3 5 Affinity Capture-Western, Co-fractionation, Affinity Capture-Western, Affinity Capture-Western, Protein-peptide
VMA3 YEL027W Proteolipid subunit c of the V0 domain of vacuolar H(+)-ATPase physical 5 3 5 Affinity Capture-Western, Co-fractionation, Affinity Capture-Western, Affinity Capture-Western, Co-purification
RAV1 YJR033C Subunit of RAVE complex (Rav1p, Rav2p, Skp1p) physical 5 2 3 Two-hybrid, Reconstituted Complex, Two-hybrid, Reconstituted Complex, Reconstituted Complex
VMA10 YHR039C-A Subunit G of the V1 peripheral membrane domain of V-ATPase physical 4 3 4 Affinity Capture-Western, Affinity Capture-Western, Protein-peptide, Co-crystal Structure
VMA8 YEL051W Subunit D of the V1 peripheral membrane domain of V-ATPase physical 4 3 4 Affinity Capture-Western, Co-fractionation, PCA, Affinity Capture-Western
VMA11 YPL234C Vacuolar ATPase V0 domain subunit c' physical 4 3 4 Affinity Capture-Western, Co-fractionation, Affinity Capture-Western, Co-purification
VMA21 YGR105W Integral membrane protein required for V-ATPase function physical 4 3 4 Affinity Capture-MS, Affinity Capture-Western, PCA, Affinity Capture-Western
FAB1 YFR019W 1-phosphatidylinositol-3-phosphate 5-kinase genetic 4 3 3 Positive Genetic, Positive Genetic, Synthetic Rescue, Phenotypic Suppression
VAM3 YOR106W Syntaxin-like vacuolar t-SNARE genetic 4 1 3 Positive Genetic, Positive Genetic, Positive Genetic, Positive Genetic
CHK1 YBR274W Serine/threonine kinase and DNA damage checkpoint effector genetic 4 1 2 Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic
YPT6 YLR262C Rab family GTPase genetic 4 1 2 Negative Genetic, Negative Genetic, Negative Genetic, Negative Genetic
VPH1 YOR270C Subunit a of the vacuolar-ATPase V0 domain physical 3 3 3 Protein-peptide, Affinity Capture-Western, Reconstituted Complex
VMA16 YHR026W Subunit c'' of the vacuolar ATPase physical 3 2 3 Affinity Capture-Western, Affinity Capture-Western, Co-purification
VMA9 YCL005W-A Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex physical 3 2 3 Affinity Capture-Western, Affinity Capture-Western, Co-purification
RGP1 YDR137W Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) genetic 3 1 3 Negative Genetic, Negative Genetic, Negative Genetic
APM3 YBR288C Mu3-like subunit of the clathrin associated protein complex (AP-3) genetic 3 2 2 Positive Genetic, Synthetic Rescue, Synthetic Rescue
GCS1 YDL226C ADP-ribosylation factor GTPase activating protein (ARF GAP) genetic 3 2 2 Negative Genetic, Negative Genetic, Positive Genetic
IDH2 YOR136W Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
SKI3 YPR189W Ski complex component and TPR protein genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
TSA2 YDR453C Stress inducible cytoplasmic thioredoxin peroxidase genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
ACS2 YLR153C Acetyl-coA synthetase isoform genetic 3 1 2 Synthetic Growth Defect, Synthetic Growth Defect, Synthetic Growth Defect
PRR1 YKL116C Serine/threonine protein kinase genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
YPT10 YBR264C Rab family GTP-binding protein genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
ZRC1 YMR243C Vacuolar membrane zinc transporter genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
VPS24 YKL041W One of four subunits of the ESCRT-III complex genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
ERV46 YAL042W Protein localized to COPII-coated vesicles genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
EDS1 YBR033W Putative zinc cluster protein, predicted to be a transcription factor genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
VHC1 YBR235W Vacuolar membrane cation-chloride cotransporter (CCC) genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
MYG1 YER156C Protein involved in mitochondrial function genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
RIM9 YMR063W Plasma membrane protein of unknown function genetic 3 1 2 Negative Genetic, Negative Genetic, Negative Genetic
VPH2 YKL119C Integral membrane protein required for V-ATPase function physical 2 2 2 Affinity Capture-Western, Affinity Capture-MS
PMC1 YGL006W Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions physical 2 2 2 PCA, Affinity Capture-Western
SFP1 YLR403W Regulates transcription of ribosomal protein and biogenesis genes regulation 2 1 2 DNA to cDNA expression microarray evidence, DNA to cDNA expression microarray evidence
APL6 YGR261C Beta3-like subunit of the yeast AP-3 complex genetic 2 1 2 Positive Genetic, Positive Genetic
ANP1 YEL036C Subunit of the alpha-1,6 mannosyltransferase complex genetic 2 1 2 Negative Genetic, Negative Genetic
VMA1 YDL185W Subunit A of the V1 peripheral membrane domain of V-ATPase genetic 2 1 2 Positive Genetic, Positive Genetic
VAM6 YDL077C Guanine nucleotide exchange factor for the GTPase Gtr1p genetic 2 1 2 Positive Genetic, Positive Genetic
CNB1 YKL190W Calcineurin B genetic 2 1 2 Negative Genetic, Negative Genetic
DOG1 YHR044C 2-deoxyglucose-6-phosphate phosphatase genetic 2 1 2 Negative Genetic, Negative Genetic
KSS1 YGR040W Mitogen-activated protein kinase (MAPK) genetic 2 1 2 Negative Genetic, Negative Genetic
DUO1 YGL061C Essential subunit of the Dam1 complex (aka DASH complex) genetic 2 1 2 Negative Genetic, Negative Genetic
IPK1 YDR315C Inositol 1,3,4,5,6-pentakisphosphate 2-kinase genetic 2 1 2 Negative Genetic, Negative Genetic
IRE1 YHR079C Serine-threonine kinase and endoribonuclease genetic 2 1 2 Negative Genetic, Negative Genetic
RIM101 YHL027W Cys2His2 zinc-finger transcriptional repressor genetic 2 1 2 Negative Genetic, Negative Genetic
SUS1 YBR111W-A Global transcriptional regulator, component of SAGA, TREX-2 complexes genetic 2 1 2 Negative Genetic, Negative Genetic
VPS27 YNR006W Endosomal protein that forms a complex with Hse1p genetic 2 1 2 Negative Genetic, Negative Genetic
VPS21 YOR089C Endosomal Rab family GTPase genetic 2 1 2 Negative Genetic, Negative Genetic
FIR1 YER032W Protein involved in 3' mRNA processing genetic 2 1 2 Negative Genetic, Negative Genetic
GDA1 YEL042W Guanosine diphosphatase located in the Golgi genetic 2 1 2 Negative Genetic, Negative Genetic
RIM21 YNL294C pH sensor molecule, component of the RIM101 pathway genetic 2 1 2 Negative Genetic, Negative Genetic
FRE1 YLR214W Ferric reductase and cupric reductase genetic 2 1 2 Negative Genetic, Negative Genetic
ALG9 YNL219C Mannosyltransferase, involved in N-linked glycosylation genetic 2 1 2 Negative Genetic, Negative Genetic
PAU12 YGR294W Protein of unknown function genetic 2 1 2 Negative Genetic, Negative Genetic
VMA6 YLR447C Subunit d of the V0 integral membrane domain of V-ATPase genetic 2 2 1 Synthetic Rescue, Phenotypic Suppression
ESA1 YOR244W Catalytic subunit of the histone acetyltransferase complex (NuA4) genetic 2 1 1 Synthetic Growth Defect, Synthetic Growth Defect
DPB3 YBR278W Third-largest subunit of DNA polymerase II (DNA polymerase epsilon) genetic 2 1 1 Negative Genetic, Negative Genetic
RPD3 YNL330C Histone deacetylase, component of both the Rpd3S and Rpd3L complexes genetic 2 1 1 Dosage Lethality, Dosage Lethality
BIT2 YBR270C Subunit of TORC2 membrane-associated complex genetic 2 1 1 Negative Genetic, Negative Genetic
OCA2 YNL056W Protein of unknown function genetic 2 1 1 Negative Genetic, Negative Genetic
ELO3 YLR372W Elongase genetic 2 1 1 Positive Genetic, Positive Genetic
YDJ1 YNL064C Type I HSP40 co-chaperone genetic 2 1 1 Negative Genetic, Negative Genetic
UBP8 YMR223W Ubiquitin-specific protease component of the SAGA acetylation complex genetic 2 1 1 Negative Genetic, Negative Genetic
PMR1 YGL167C High affinity Ca2+/Mn2+ P-type ATPase genetic 2 1 1 Positive Genetic, Positive Genetic
SCH9 YHR205W AGC family protein kinase genetic 2 1 1 Synthetic Growth Defect, Synthetic Growth Defect
HUR1 YGL168W Protein with a role in DNA repair genetic 2 1 1 Positive Genetic, Positive Genetic
PCK1 YKR097W Phosphoenolpyruvate carboxykinase genetic 2 1 1 Positive Genetic, Positive Genetic
SET6 YPL165C SET domain protein of unknown function genetic 2 1 1 Negative Genetic, Negative Genetic
MIC60 YKR016W Component of the MICOS complex genetic 2 1 1 Negative Genetic, Negative Genetic
FKH1 YIL131C Forkhead family transcription factor regulation 1 1 1 chromatin immunoprecipitation-chip evidence
IXR1 YKL032C Transcriptional repressor that regulates hypoxic genes during normoxia regulation 1 1 1 DNA to cDNA expression microarray evidence
FKH2 YNL068C Forkhead family transcription factor regulation 1 1 1 chromatin immunoprecipitation-chip evidence
ASH1 YKL185W Component of the Rpd3L histone deacetylase complex regulation 1 1 1 chromatin immunoprecipitation-chip evidence
RPF1 YHR088W Protein involved in assembly and export of the large ribosomal subunit genetic 1 1 1 Negative Genetic
RPF2 YKR081C Essential protein involved in rRNA maturation and ribosomal assembly genetic 1 1 1 Negative Genetic
RPL10 YLR075W Ribosomal 60S subunit protein L10 genetic 1 1 1 Negative Genetic
RPL16A YIL133C Ribosomal 60S subunit protein L16A physical 1 1 1 Affinity Capture-MS
RIC1 YLR039C Protein involved in retrograde transport to the cis-Golgi network genetic 1 1 1 Negative Genetic
POL5 YEL055C Protein involved in 60S assembly and pre-40S AFs recycling genetic 1 1 1 Negative Genetic
MES1 YGR264C Methionyl-tRNA synthetase genetic 1 1 1 Negative Genetic
BMH1 YER177W 14-3-3 protein, major isoform genetic 1 1 1 Negative Genetic
LSM1 YJL124C Lsm (Like Sm) protein genetic 1 1 1 Negative Genetic
DIS3 YOL021C Exosome core complex catalytic subunit genetic 1 1 1 Negative Genetic
RRP42 YDL111C Exosome non-catalytic core component genetic 1 1 1 Negative Genetic
RPA34 YJL148W RNA polymerase I subunit A34.5 genetic 1 1 1 Negative Genetic
RPB5 YBR154C RNA polymerase subunit ABC27 genetic 1 1 1 Negative Genetic
RET1 YOR207C Second-largest subunit of RNA polymerase III physical 1 1 1 Affinity Capture-MS
RPC25 YKL144C RNA polymerase III subunit C25 genetic 1 1 1 Negative Genetic
ARP9 YMR033W Component of both the SWI/SNF and RSC chromatin remodeling complexes genetic 1 1 1 Positive Genetic
NAP1 YKR048C Histone chaperone and assembly chaperone for Rps6a/b genetic 1 1 1 Negative Genetic
CEX1 YOR112W Component of nuclear aminoacylation-dependent tRNA export pathway physical 1 1 1 Affinity Capture-MS
VIP1 YLR410W Bifunctional inositol pyrophosphate kinase and phosphatase genetic 1 1 1 Negative Genetic
RPN1 YHR027C Non-ATPase base subunit of the 19S RP of the 26S proteasome physical 1 1 1 Affinity Capture-MS
RPN10 YHR200W Proteasome polyubiquitin receptor physical 1 1 1 Affinity Capture-MS
RPN11 YFR004W Metalloprotease subunit of 19S regulatory particle physical 1 1 1 Affinity Capture-MS
RPT2 YDL007W ATPase of the 19S regulatory particle of the 26S proteasome genetic 1 1 1 Negative Genetic
RPG1 YBR079C eIF3a subunit of the eukaryotic translation initiation factor 3 (eIF3) genetic 1 1 1 Positive Genetic
TIF34 YMR146C eIF3i subunit of the eukaryotic translation initiation factor 3 (eIF3) genetic 1 1 1 Positive Genetic
ACT1 YFL039C Actin, structural protein in cytoskeleton genetic 1 1 1 Negative Genetic
IMD3 YLR432W Inosine monophosphate dehydrogenase genetic 1 1 1 Negative Genetic
AAR2 YBL074C Component of the U5 snRNP complex genetic 1 1 1 Negative Genetic
CDC39 YCR093W Subunit of the CCR4-NOT1 core complex genetic 1 1 1 Negative Genetic
NAB2 YGL122C Nuclear polyadenylated RNA-binding protein physical 1 1 1 Affinity Capture-RNA
PEP1 YBL017C Type I transmembrane sorting receptor for multiple vacuolar hydrolases genetic 1 1 1 Negative Genetic
ABD1 YBR236C Methyltransferase genetic 1 1 1 Negative Genetic
AOS1 YPR180W Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Uba2p genetic 1 1 1 Negative Genetic
APL5 YPL195W Delta adaptin-like subunit of the clathrin associated protein complex genetic 1 1 1 Positive Genetic
BEM1 YBR200W Protein containing SH3-domains genetic 1 1 1 Negative Genetic
ASC1 YMR116C G-protein beta subunit and guanine dissociation inhibitor for Gpa2p genetic 1 1 1 Negative Genetic
BUD14 YAR014C Protein involved in bud-site selection genetic 1 1 1 Positive Genetic
CAF130 YGR134W Subunit of the CCR4-NOT transcriptional regulatory complex genetic 1 1 1 Negative Genetic
CDC12 YHR107C Component of the septin ring that is required for cytokinesis genetic 1 1 1 Positive Genetic
RIX1 YHR197W Component of the Rix1 complex and possibly pre-replicative complexes genetic 1 1 1 Negative Genetic
BUD31 YCR063W Component of the SF3b subcomplex of the U2 snRNP genetic 1 1 1 Positive Genetic
COP1 YDL145C Alpha subunit of COPI vesicle coatomer complex genetic 1 1 1 Negative Genetic
CPR1 YDR155C Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin) genetic 1 1 1 Negative Genetic
DCP1 YOL149W Subunit of the Dcp1p-Dcp2p decapping enzyme complex genetic 1 1 1 Negative Genetic
ENO1 YGR254W Enolase I, a phosphopyruvate hydratase genetic 1 1 1 Negative Genetic
ERO1 YML130C Thiol oxidase required for oxidative protein folding in the ER genetic 1 1 1 Negative Genetic
FTH1 YBR207W Putative high affinity iron transporter physical 1 1 1 PCA
GLO3 YER122C ADP-ribosylation factor GTPase activating protein (ARF GAP) genetic 1 1 1 Negative Genetic
DHH1 YDL160C Cytoplasmic DEAD-box helicase and mRNA decapping activator physical 1 1 1 Affinity Capture-RNA
LCB1 YMR296C Component of serine palmitoyltransferase genetic 1 1 1 Negative Genetic
LCB2 YDR062W Component of serine palmitoyltransferase genetic 1 1 1 Negative Genetic
MRPL19 YNL185C Mitochondrial ribosomal protein of the large subunit genetic 1 1 1 Positive Genetic
MRPL24 YMR193W Mitochondrial ribosomal protein of the large subunit genetic 1 1 1 Positive Genetic
HDA3 YPR179C Subunit of HDA1 histone deacetylase complex genetic 1 1 1 Negative Genetic
CMR1 YDL156W Nuclear protein with role in transcription, protein quality control genetic 1 1 1 Negative Genetic
INO80 YGL150C Nucleosome spacing factor genetic 1 1 1 Negative Genetic
NUP60 YAR002W FG-nucleoporin component of central core of the nuclear pore complex genetic 1 1 1 Synthetic Growth Defect
GIM3 YNL153C Subunit of the heterohexameric cochaperone prefoldin complex genetic 1 1 1 Negative Genetic
GIM5 YML094W Subunit of the heterohexameric cochaperone prefoldin complex genetic 1 1 1 Negative Genetic
POP7 YBR167C Subunit of RNase MRP, nuclear RNase P and telomerase genetic 1 1 1 Negative Genetic
HSP82 YPL240C Hsp90 chaperone genetic 1 1 1 Synthetic Lethality
LEA1 YPL213W Component of U2 snRNP complex genetic 1 1 1 Negative Genetic
CWC22 YGR278W Spliceosome-associated protein that is required for pre-mRNA splicing genetic 1 1 1 Negative Genetic
DAM1 YGR113W Essential subunit of the Dam1 complex (aka DASH complex) genetic 1 1 1 Negative Genetic
PDC1 YLR044C Major of three pyruvate decarboxylase isozymes physical 1 1 1 Affinity Capture-MS
RAD23 YEL037C Protein with ubiquitin-like N terminus genetic 1 1 1 Dosage Growth Defect
RET2 YFR051C Delta subunit of the coatomer complex (COPI) genetic 1 1 1 Negative Genetic
CSI1 YMR025W Subunit of the Cop9 signalosome genetic 1 1 1 Negative Genetic
NUM1 YDR150W Protein required for nuclear migration genetic 1 1 1 Synthetic Growth Defect
RSA4 YCR072C Ribosomal large subunit (60S) assembly factor genetic 1 1 1 Negative Genetic
PGI1 YBR196C Glycolytic enzyme phosphoglucose isomerase physical 1 1 1 Affinity Capture-MS
SEC26 YDR238C Essential beta-coat protein of the COPI coatomer genetic 1 1 1 Negative Genetic
SEC66 YBR171W Non-essential subunit of Sec63 complex genetic 1 1 1 Negative Genetic
SET2 YJL168C Histone methyltransferase with a role in transcriptional elongation genetic 1 1 1 Negative Genetic
SET3 YKR029C Defining member of the SET3 histone deacetylase complex genetic 1 1 1 Positive Genetic
CDC25 YLR310C Membrane bound guanine nucleotide exchange factor genetic 1 1 1 Negative Genetic
SCP160 YJL080C Essential RNA-binding G protein effector of mating response pathway physical 1 1 1 Affinity Capture-MS
PTC1 YDL006W Type 2C protein phosphatase (PP2C) genetic 1 1 1 Negative Genetic
SBP1 YHL034C Protein that binds eIF4G and has a role in repression of translation physical 1 1 1 Affinity Capture-RNA
SBP1 YHL034C Protein that binds eIF4G and has a role in repression of translation genetic 1 1 1 Negative Genetic
SKN1 YGR143W Protein involved in sphingolipid biosynthesis genetic 1 1 1 Positive Genetic
RPT4 YOR259C ATPase of the 19S regulatory particle of the 26S proteasome physical 1 1 1 PCA
HSE1 YHL002W Subunit of the endosomal Vps27p-Hse1p complex genetic 1 1 1 Negative Genetic
TLG2 YOL018C Syntaxin-like t-SNARE genetic 1 1 1 Negative Genetic
PUF3 YLL013C Protein of the mitochondrial outer surface physical 1 1 1 Affinity Capture-RNA
LYS9 YNR050C Saccharopine dehydrogenase (NADP+, L-glutamate-forming) physical 1 1 1 Affinity Capture-MS
PYC2 YBR218C Pyruvate carboxylase isoform genetic 1 1 1 Negative Genetic
CAF20 YOR276W Phosphoprotein of the mRNA cap-binding complex physical 1 1 1 Affinity Capture-MS
DCS1 YLR270W Non-essential hydrolase involved in mRNA decapping genetic 1 1 1 Negative Genetic
PRB1 YEL060C Vacuolar proteinase B (yscB) with H3 N-terminal endopeptidase activity genetic 1 1 1 Negative Genetic
RHO5 YNL180C Non-essential small GTPase of the Rho/Rac family of Ras-like proteins genetic 1 1 1 Synthetic Rescue
RVS161 YCR009C Amphiphysin-like lipid raft protein genetic 1 1 1 Negative Genetic
NOP6 YDL213C rRNA-binding protein required for 40S ribosomal subunit biogenesis physical 1 1 1 Affinity Capture-MS
PMA1 YGL008C Plasma membrane P2-type H+-ATPase genetic 1 1 1 Negative Genetic
ESL2 YKR096W hEST1A/B (SMG5/6)-like protein genetic 1 1 1 Positive Genetic
PDI1 YCL043C Protein disulfide isomerase genetic 1 1 1 Negative Genetic
ATG11 YPR049C Adapter protein for pexophagy and the Cvt targeting pathway genetic 1 1 1 Negative Genetic
HSP104 YLL026W Disaggregase physical 1 1 1 Affinity Capture-MS
ATG42 YBR139W Vacuolar serine-type carboxypeptidase genetic 1 1 1 Negative Genetic
AYR1 YIL124W Bifunctional triacylglycerol lipase and 1-acyl DHAP reductase genetic 1 1 1 Negative Genetic
DLD3 YEL071W 2-hydroxyglutarate transhydrogenase, and minor D-lactate dehydrogenase genetic 1 1 1 Negative Genetic
DSK2 YMR276W Nuclear-enriched ubiquitin-like polyubiquitin-binding protein genetic 1 1 1 Dosage Growth Defect
RSP5 YER125W NEDD4 family E3 ubiquitin ligase genetic 1 1 1 Negative Genetic
POL30 YBR088C Proliferating cell nuclear antigen (PCNA) genetic 1 1 1 Negative Genetic
GPN3 YLR243W Putative GTPase with a role in biogenesis of RNA pol II and polIII genetic 1 1 1 Negative Genetic
HOM6 YJR139C Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase) genetic 1 1 1 Negative Genetic
RAD6 YGL058W Ubiquitin-conjugating enzyme (E2) genetic 1 1 1 Negative Genetic
NTF2 YER009W Nuclear envelope protein genetic 1 1 1 Negative Genetic
DNL4 YOR005C DNA ligase required for nonhomologous end-joining (NHEJ) genetic 1 1 1 Positive Genetic
HOG1 YLR113W Mitogen-activated protein kinase involved in osmoregulation genetic 1 1 1 Synthetic Growth Defect
KSP1 YHR082C Serine/threonine protein kinase genetic 1 1 1 Negative Genetic
EMI2 YDR516C Hexokinase expressed during growth with low glucose levels physical 1 1 1 Affinity Capture-MS
TMA108 YIL137C Ribosome-associated, nascent chain binding factor physical 1 1 1 Affinity Capture-MS
RAD50 YNL250W Subunit of MRX complex with Mre11p and Xrs2p genetic 1 1 1 Negative Genetic
PGM1 YKL127W Phosphoglucomutase, minor isoform genetic 1 1 1 Positive Genetic
THI21 YPL258C Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase genetic 1 1 1 Negative Genetic
GBP2 YCL011C Poly(A+) RNA-binding protein genetic 1 1 1 Negative Genetic
APE2 YKL157W Aminopeptidase yscII genetic 1 1 1 Positive Genetic
PHO86 YJL117W Endoplasmic reticulum (ER) resident protein genetic 1 1 1 Negative Genetic
BIR1 YJR089W Subunit of chromosomal passenger complex (CPC) genetic 1 1 1 Negative Genetic
MMS22 YLR320W Subunit of E3 ubiquitin ligase complex involved in replication repair physical 1 1 1 Affinity Capture-MS
IME2 YJL106W Serine/threonine protein kinase involved in activation of meiosis genetic 1 1 1 Negative Genetic
MTQ2 YDR140W S-adenosylmethionine-dependent methyltransferase physical 1 1 1 Affinity Capture-MS
UBA4 YHR111W E1-like protein that activates Urm1p before urmylation genetic 1 1 1 Negative Genetic
DAL82 YNL314W Positive regulator of allophanate inducible genes genetic 1 1 1 Positive Genetic
BIM1 YER016W Microtubule plus end-tracking protein genetic 1 1 1 Negative Genetic
FIG1 YBR040W Integral membrane protein required for efficient mating genetic 1 1 1 Negative Genetic
GTR1 YML121W Subunit of a TORC1-stimulating GTPase and the EGO/GSE complex physical 1 1 1 Affinity Capture-Western
SMT3 YDR510W Ubiquitin-like protein of the SUMO family physical 1 1 1 PCA
MCM16 YPR046W Component of the Ctf19 complex and the COMA subcomplex genetic 1 1 1 Negative Genetic
GNP1 YDR508C Broad specificity amino acid permease genetic 1 1 1 Negative Genetic
STB2 YMR053C Protein that interacts with Sin3p in a two-hybrid assay genetic 1 1 1 Negative Genetic
VAM7 YGL212W Vacuolar SNARE protein genetic 1 1 1 Positive Genetic
VMA22 YHR060W Protein that is required for vacuolar H+-ATPase (V-ATPase) function physical 1 1 1 Affinity Capture-Western
KEG1 YFR042W Integral membrane protein of the ER genetic 1 1 1 Negative Genetic
LAM6 YLR072W Sterol transporter that transfers sterols between membranes physical 1 1 1 Affinity Capture-MS
SAM35 YHR083W Component of the sorting and assembly machinery (SAM) complex genetic 1 1 1 Negative Genetic
ELP6 YMR312W Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex genetic 1 1 1 Negative Genetic
ELP4 YPL101W Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex genetic 1 1 1 Negative Genetic
PEX17 YNL214W Membrane peroxin of the peroxisomal importomer complex genetic 1 1 1 Positive Genetic
RLP24 YLR009W Essential protein required for ribosomal large subunit biogenesis genetic 1 1 1 Negative Genetic
RPL23B YER117W Ribosomal 60S subunit protein L23B genetic 1 1 1 Negative Genetic
SWC7 YLR385C Protein of unknown function genetic 1 1 1 Negative Genetic
DAL81 YIR023W Positive regulator of genes in multiple nitrogen degradation pathways genetic 1 1 1 Negative Genetic
RPS11A YDR025W Protein component of the small (40S) ribosomal subunit genetic 1 1 1 Negative Genetic
ATG8 YBL078C Ub-like protein physical 1 1 1 Co-localization
BFR1 YOR198C Component of mRNP complexes associated with polyribosomes physical 1 1 1 Affinity Capture-RNA
CLC1 YGR167W Clathrin light chain genetic 1 1 1 Positive Genetic
ELO2 YCR034W Fatty acid elongase, involved in sphingolipid biosynthesis genetic 1 1 1 Positive Genetic
GIM4 YEL003W Subunit of the heterohexameric cochaperone prefoldin complex genetic 1 1 1 Negative Genetic
MCM3 YEL032W Protein involved in DNA replication genetic 1 1 1 Negative Genetic
OST3 YOR085W Gamma subunit of the oligosaccharyltransferase complex of the ER lumen genetic 1 1 1 Negative Genetic
SEC61 YLR378C Conserved ER protein translocation channel genetic 1 1 1 Negative Genetic
SEC22 YLR268W R-SNARE protein genetic 1 1 1 Negative Genetic
TUB4 YLR212C Gamma-tubulin genetic 1 1 1 Synthetic Growth Defect
VTC1 YER072W Regulatory subunit of the vacuolar transporter chaperone (VTC) complex physical 1 1 1 Affinity Capture-Western
VTC4 YJL012C Vacuolar membrane polyphosphate polymerase physical 1 1 1 Affinity Capture-Western
YOR1 YGR281W Plasma membrane ATP-binding cassette (ABC) transporter physical 1 1 1 Affinity Capture-MS
YRA1 YDR381W Nuclear polyadenylated RNA-binding protein physical 1 1 1 Affinity Capture-MS
VPS4 YPR173C AAA-ATPase involved in multivesicular body (MVB) protein sorting genetic 1 1 1 Negative Genetic
YDL157C YDL157C Putative protein of unknown function genetic 1 1 1 Positive Genetic
YKE2 YLR200W Subunit of the heterohexameric Gim/prefoldin protein complex genetic 1 1 1 Negative Genetic
YPT7 YML001W Rab family GTPase genetic 1 1 1 Positive Genetic
YMD8 YML038C Putative nucleotide sugar transporter genetic 1 1 1 Negative Genetic
VPS60 YDR486C Protein involved in late endosome to vacuole transport genetic 1 1 1 Negative Genetic
VPS1 YKR001C Dynamin-like GTPase required for vacuolar sorting genetic 1 1 1 Negative Genetic
VPS38 YLR360W Part of a Vps34p phosphatidylinositol 3-kinase complex genetic 1 1 1 Negative Genetic
YTH1 YPR107C Essential RNA-binding component of cleavage and polyadenylation factor genetic 1 1 1 Positive Genetic
YGL114W YGL114W Putative protein of unknown function genetic 1 1 1 Positive Genetic
YIR016W YIR016W Putative protein of unknown function genetic 1 1 1 Positive Genetic
YPT1 YFL038C Rab family GTPase genetic 1 1 1 Negative Genetic
YEA4 YEL004W Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter genetic 1 1 1 Negative Genetic
YER152C YER152C Protein with 2-aminoadipate transaminase activity genetic 1 1 1 Negative Genetic
VPS45 YGL095C Protein of the Sec1p/Munc-18 family genetic 1 1 1 Negative Genetic
YCH1 YGR203W Phosphatase with sequence similarity to Mih1p genetic 1 1 1 Negative Genetic
YGR283C YGR283C Putative methyltransferase genetic 1 1 1 Negative Genetic
YHR045W YHR045W Putative protein of unknown function genetic 1 1 1 Negative Genetic
YJR011C YJR011C Putative protein of unknown function genetic 1 1 1 Negative Genetic
YKR015C YKR015C Putative protein of unknown function genetic 1 1 1 Negative Genetic
XYL2 YLR070C Xylitol dehydrogenase genetic 1 1 1 Negative Genetic
YLR179C YLR179C Protein of unknown function with similarity to Tfs1p genetic 1 1 1 Negative Genetic
VPS36 YLR417W Component of the ESCRT-II complex genetic 1 1 1 Negative Genetic
YML037C YML037C Putative protein of unknown function genetic 1 1 1 Negative Genetic
YMR321C YMR321C Putative protein of unknown function genetic 1 1 1 Negative Genetic
YNL194C YNL194C Integral membrane protein genetic 1 1 1 Negative Genetic
YOL159C-A YOL159C-A Protein of unknown function genetic 1 1 1 Negative Genetic
YPF1 YKL100C Intramembrane aspartyl protease of the perinuclear ER membrane genetic 1 1 1 Negative Genetic
STP22 YCL008C Component of the ESCRT-I complex genetic 1 1 1 Negative Genetic
SRN2 YLR119W Component of the ESCRT-I complex genetic 1 1 1 Negative Genetic
CST9 YLR394W SUMO E3 ligase genetic 1 1 1 Negative Genetic
GOS1 YHL031C v-SNARE protein involved in Golgi transport genetic 1 1 1 Positive Genetic
RSM19 YNR037C Mitochondrial ribosomal protein of the small subunit genetic 1 1 1 Negative Genetic
GYP1 YOR070C Cis-golgi GTPase-activating protein (GAP) for yeast Rabs genetic 1 1 1 Negative Genetic
POL3 YDL102W Catalytic subunit of DNA polymerase delta genetic 1 1 1 Negative Genetic
FCP1 YMR277W Carboxy-terminal domain (CTD) phosphatase genetic 1 1 1 Positive Genetic
STE50 YCL032W Adaptor protein for various signaling pathways genetic 1 1 1 Negative Genetic
PRP16 YKR086W DEAH-box RNA helicase genetic 1 1 1 Negative Genetic
NNF2 YGR089W Protein that exhibits physical and genetic interactions with Rpb8p genetic 1 1 1 Negative Genetic
IVY1 YDR229W Phospholipid-binding protein that interacts with both Ypt7p and Vps33p genetic 1 1 1 Synthetic Growth Defect
MGA1 YGR249W Protein similar to heat shock transcription factor genetic 1 1 1 Negative Genetic
RPL1B YGL135W Ribosomal 60S subunit protein L1B genetic 1 1 1 Negative Genetic
SCW4 YGR279C Cell wall protein with similarity to glucanases genetic 1 1 1 Negative Genetic
AUR1 YKL004W Phosphatidylinositol:ceramide phosphoinositol transferase genetic 1 1 1 Negative Genetic
BSD2 YBR290W Heavy metal ion homeostasis protein genetic 1 1 1 Negative Genetic
HOC1 YJR075W Alpha-1,6-mannosyltransferase genetic 1 1 1 Negative Genetic
MNN11 YJL183W Subunit of a Golgi mannosyltransferase complex genetic 1 1 1 Negative Genetic
HAC1 YFL031W Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog) genetic 1 1 1 Negative Genetic
STV1 YMR054W Subunit a of the vacuolar-ATPase V0 domain physical 1 1 1 Affinity Capture-Western
UBX7 YBR273C UBX (ubiquitin regulatory X) domain-containing protein genetic 1 1 1 Positive Genetic
MON2 YNL297C Protein with a role in endocytosis and vacuole integrity genetic 1 1 1 Negative Genetic
EAF6 YJR082C Subunit of the NuA4 acetyltransferase complex genetic 1 1 1 Negative Genetic
SYS1 YJL004C Integral membrane protein of the Golgi genetic 1 1 1 Negative Genetic
TAO3 YIL129C Component of the RAM signaling network genetic 1 1 1 Negative Genetic
IXR1 YKL032C Transcriptional repressor that regulates hypoxic genes during normoxia physical 1 1 1 Affinity Capture-MS
RPL15A YLR029C Ribosomal 60S subunit protein L15A genetic 1 1 1 Negative Genetic
MEU1 YLR017W Methylthioadenosine phosphorylase (MTAP) genetic 1 1 1 Negative Genetic
SAC7 YDR389W GTPase activating protein (GAP) for Rho1p genetic 1 1 1 Negative Genetic
GTA1 YEL043W Protein of unknown function genetic 1 1 1 Negative Genetic
ARR3 YPR201W Plasma membrane metalloid/H+ antiporter genetic 1 1 1 Negative Genetic
PSG1 YKL077W Protein that promotes the transport and maturation of Pma1 with Exp1 physical 1 1 1 Affinity Capture-MS
VMA21 YGR105W Integral membrane protein required for V-ATPase function genetic 1 1 1 Synthetic Growth Defect
DIC1 YLR348C Mitochondrial dicarboxylate carrier genetic 1 1 1 Negative Genetic
LYS20 YDL182W Homocitrate synthase isozyme and functions in DNA repair genetic 1 1 1 Negative Genetic
SWD3 YBR175W Essential subunit of the COMPASS (Set1C) complex genetic 1 1 1 Negative Genetic
NGR1 YBR212W RNA binding protein that negatively regulates growth rate genetic 1 1 1 Negative Genetic
NFS1 YCL017C Cysteine desulfurase involved in iron-sulfur cluster biogenesis genetic 1 1 1 Positive Genetic
UME6 YDR207C Rpd3L histone deacetylase complex subunit genetic 1 1 1 Negative Genetic
ICL1 YER065C Isocitrate lyase genetic 1 1 1 Positive Genetic
TSA1 YML028W Thioredoxin peroxidase genetic 1 1 1 Synthetic Lethality
UBP2 YOR124C Ubiquitin-specific protease genetic 1 1 1 Negative Genetic
RIM8 YGL045W Protein involved in proteolytic activation of Rim101p genetic 1 1 1 Negative Genetic
MVB12 YGR206W ESCRT-I subunit required to stabilize ESCRT-I core complex oligomers genetic 1 1 1 Negative Genetic
RPP1A YDL081C Ribosomal stalk protein P1 alpha genetic 1 1 1 Negative Genetic
RTT109 YLL002W Histone acetyltransferase genetic 1 1 1 Synthetic Growth Defect
RIA1 YNL163C Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis genetic 1 1 1 Negative Genetic
SMT1 YJL147C Translational repressor of the mitochondrial ATP6/8 mRNA genetic 1 1 1 Positive Genetic
RPS25B YLR333C Protein component of the small (40S) ribosomal subunit genetic 1 1 1 Negative Genetic
VAC14 YLR386W Enzyme regulator genetic 1 1 1 Synthetic Rescue
PSP2 YML017W Asn rich cytoplasmic protein that contains RGG motifs genetic 1 1 1 Negative Genetic
PKR1 YMR123W V-ATPase assembly factor genetic 1 1 1 Synthetic Growth Defect
PGS1 YCL004W Phosphatidylglycerolphosphate synthase genetic 1 1 1 Negative Genetic
TMA46 YOR091W Protein of unknown function that associates with translating ribosomes physical 1 1 1 Affinity Capture-MS
MUB1 YMR100W MYND domain-containing protein genetic 1 1 1 Negative Genetic
TUB2 YFL037W Beta-tubulin genetic 1 1 1 Negative Genetic
SEH1 YGL100W Subunit of the Nup84 nuclear pore and SEACAT subcomplexes genetic 1 1 1 Positive Genetic
SND1 YDR186C Protein involved in SRP-independent targeting of substrates to the ER genetic 1 1 1 Negative Genetic
GPI17 YDR434W Transmembrane protein genetic 1 1 1 Negative Genetic
IPT1 YDR072C Inositolphosphotransferase genetic 1 1 1 Positive Genetic
BST1 YFL025C GPI inositol deacylase of the endoplasmic reticulum (ER) genetic 1 1 1 Positive Genetic
ARO2 YGL148W Bifunctional chorismate synthase and flavin reductase genetic 1 1 1 Negative Genetic
APJ1 YNL077W Hsp40 chaperone with a role in SUMO-mediated protein degradation physical 1 1 1 Affinity Capture-MS
PIK1 YNL267W Phosphatidylinositol 4-kinase genetic 1 1 1 Synthetic Growth Defect
LOA1 YPR139C Lysophosphatidic acid acyltransferase physical 1 1 1 Co-fractionation
VCX1 YDL128W Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity genetic 1 1 1 Synthetic Growth Defect
RPL14A YKL006W Ribosomal 60S subunit protein L14A genetic 1 1 1 Negative Genetic
MNN2 YBR015C Alpha-1,2-mannosyltransferase genetic 1 1 1 Negative Genetic
CDC50 YCR094W Endosomal protein that interacts with phospholipid flippase Drs2p genetic 1 1 1 Positive Genetic
FMP45 YDL222C Integral membrane protein localized to mitochondria genetic 1 1 1 Negative Genetic
RIM13 YMR154C Calpain-like cysteine protease genetic 1 1 1 Negative Genetic
MDM34 YGL219C Mitochondrial component of the ERMES complex genetic 1 1 1 Negative Genetic
AST2 YER101C Lipid raft associated protein genetic 1 1 1 Negative Genetic
MPS3 YJL019W Nuclear envelope protein physical 1 1 1 Affinity Capture-MS
NCB2 YDR397C Subunit of a heterodimeric NC2 transcription regulator complex genetic 1 1 1 Negative Genetic
DFG16 YOR030W Probable multiple transmembrane protein genetic 1 1 1 Negative Genetic
KIP3 YGL216W Kinesin-related antiparallel sliding motor protein genetic 1 1 1 Negative Genetic
ERG4 YGL012W C-24(28) sterol reductase genetic 1 1 1 Negative Genetic
RER2 YBR002C Forms the dehydrodolichyl diphosphate synthase (DDS) complex with NUS1 genetic 1 1 1 Positive Genetic
PMC1 YGL006W Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions genetic 1 1 1 Synthetic Growth Defect
GOT1 YMR292W Homodimeric protein that is packaged into COPII vesicles genetic 1 1 1 Positive Genetic
VOA1 YGR106C ER protein that functions in assembly of the V0 sector of V-ATPase physical 1 1 1 PCA
VOA1 YGR106C ER protein that functions in assembly of the V0 sector of V-ATPase genetic 1 1 1 Synthetic Growth Defect
ERG3 YLR056W C-5 sterol desaturase genetic 1 1 1 Positive Genetic
ARO7 YPR060C Chorismate mutase genetic 1 1 1 Negative Genetic
BSC4 YNL269W Protein of unknown function genetic 1 1 1 Negative Genetic
PHA2 YNL316C Prephenate dehydratase genetic 1 1 1 Negative Genetic
VNX1 YNL321W Calcium/H+ antiporter localized to the endoplasmic reticulum membrane genetic 1 1 1 Phenotypic Enhancement
RPO41 YFL036W Mitochondrial RNA polymerase genetic 1 1 1 Positive Genetic
MYG1 YER156C Protein involved in mitochondrial function physical 1 1 1 Proximity Label-MS
ERD1 YDR414C Predicted membrane protein required for lumenal ER protein retention genetic 1 1 1 Negative Genetic
TDA5 YLR426W Putative protein of unknown function genetic 1 1 1 Negative Genetic
DOG2 YHR043C 2-deoxyglucose-6-phosphate phosphatase genetic 1 1 1 Negative Genetic
AFG1 YEL052W Protein that may act as a chaperone for cytochrome c oxidase subunits genetic 1 1 1 Positive Genetic
IRC20 YLR247C E3 ubiquitin ligase and putative helicase genetic 1 1 1 Positive Genetic
APS3 YJL024C Small subunit of the clathrin-associated adaptor complex AP-3 genetic 1 1 1 Positive Genetic
HXT9 YJL219W Putative hexose transporter that is nearly identical to Hxt11p genetic 1 1 1 Negative Genetic
ADY2 YCR010C Acetate transporter required for normal sporulation genetic 1 1 1 Negative Genetic
VAC7 YNL054W Integral vacuolar membrane protein genetic 1 1 1 Synthetic Rescue
ALG8 YOR067C Glucosyl transferase genetic 1 1 1 Negative Genetic
ALK2 YBL009W Atypical protein kinase genetic 1 1 1 Negative Genetic
PRM7 YDL039C Pheromone-regulated protein genetic 1 1 1 Negative Genetic
EFM3 YJR129C S-adenosylmethionine-dependent methyltransferase genetic 1 1 1 Negative Genetic
SER2 YGR208W Phosphoserine phosphatase of the phosphoglycerate pathway genetic 1 1 1 Negative Genetic
MNT3 YIL014W Alpha-1,3-mannosyltransferase genetic 1 1 1 Negative Genetic
ECM14 YHR132C Conserved fungal protein with similarity to zinc carboxypeptidases genetic 1 1 1 Negative Genetic
GPI11 YDR302W ER membrane protein involved in a late step of GPI anchor assembly genetic 1 1 1 Positive Genetic
RML2 YEL050C Mitochondrial ribosomal protein of the large subunit (L2) genetic 1 1 1 Positive Genetic
RSO55 YLR281C Mitochondrial protein of unknown function genetic 1 1 1 Negative Genetic
RMD9 YGL107C Mitochondrial protein that controls mitochondrial gene expression genetic 1 1 1 Negative Genetic
TSC10 YBR265W 3-ketosphinganine reductase genetic 1 1 1 Negative Genetic
SPF1 YEL031W P-type ATPase, ion transporter of the ER membrane genetic 1 1 1 Negative Genetic
PAU11 YGL261C Putative protein of unknown function genetic 1 1 1 Negative Genetic